Antimicrobial activity of bifidobacteria isolated from baby faeces

Bifidobacteria strains, isolated from baby faeces, were examined for their antibacterial activity by deferred agar spot [DAS] test. A number of thirteen isolates were investigated using test strains from two different genera [Salmonella typhimurium and Escherichia coli]. The isolates were morphologically characterized and molecularly identified via polymerase chain reaction [PCR]. The different random amplified polymorphic DNA [RAPD] patterns were able to identify three groups of Bifidobacteria. All isolates showed an inhibition zone diameter with a mean of 10 mm against S. typhimurium and 6 mm against E. coli