RESUMEN
Aim: To determine antimicrobial and antioxidative effects of Ganoderma lucidum. Place and Duration of Study: Erciyes University, Faculty of Pharmacy, Pharmaceutical Biotechnology research laboratory,Kayseri, Turkey, between January to March, 2013. Methodology: Antimicrobial inhibitory effects were carried out on the extracts using disc diffusion method. Antioxidant activities of the ethanolic and methanolic extracts from G.lucidum were evaluated by using 2,2-diphenyl-1-picrylhydrazyl [DPPH] radical scavenging, metal chelating, total flavonoid and total antioxidant activity assays. In addition, the amounts of phenolic compound, β-carotene and lycopene components in the extracts were determined. Results: The antimicrobial effects of ethanolic and methanolic extracts of G. lucidum were tested against one species of Gram positive bacteria, two species of Gram-negative bacteria and two yeast. The highest inhibitory activity was determined against Candida glabrata RSKK 04019 [25±1 mm, inhibition zone diameter]. On the other hand, the lowest inhibitory activity was determined against Candida albicans ATCC 90028 and Bacillus subtilis RSKK 244 [10±1 and 10±0 mm, inhibition zone diameter]. DPPH radical scavenging effect was detected in the methanol extract [IC50 = 3.82±0.04 μg/mL] was higher than the ethanol extracts [IC50 = 7.03±0.07 μg/mL]. Compared to reference antioxidant, the methanol and ethanol extracts of G.lucidum provided a lower IC50 than butylated hydoxyanisole [BHA] [IC50 = 0.30±0.01 μg/mL]. Phenolic compounds were the major antioxidant component found in the mushroom extracts. Conclusion: These results showed that G. lucidum may be used in pharmaceutical applications because of its effective antioxidant properties.