Purification and characterization of pectin lyase of erwinia dissolvent

Erwinia dissolvens [Code No. E2 F2] was grown in two different media by static culture method for two weeks. The extracellular pectin lyase produced by the organism in Medium I was partially purified by gel filtration chromatography on Sephadex G-75 and Sephadex G-200 while that produced in Medium II was purified by chromatographing on Sephadex G-75, Sephadex G-200, DEAE-cellulose columns. Some characteristics of the enzyme were determined and compared with those of pectin lyases and pectate lyases of bacterial and fungal origins previously reported in the literature