Simultaneous Determination of Matrine and Oxymatrine in Human Plasma by HPLC / 中国药房

OBJECTIVE:To established a method for determining matrine and oxymatrine in human plasma.METHODS:The plasma was extracted with chloroform-n-butyl alcohol(98∶2) after basification and purified with neutral alumina solid-phase extraction.Then it was eluted with a Lichrosorb-NH2 column and CH3CN-CH3CH2OH-H3PO4(80∶10∶8),detected at ? 220nm.RESULTS:The linear range was 1.25mg～40mg/L and the limit of detection was 0.1mg/L(S/N=2).The average recoveries of matrine and oxymatrine were 106.96% and 105.04%,respectively.RSDs of within-day and between-day were lower than 13% and 7% respectively.CONCLUSION:The present study provides a simple and reliable method for determining concentrations of matrine and oxymatrine in human plasma.

Analysis of Irrational Prescriptions in Outpatient and Emergency Departments in2003 / 中国药房

OBJECTIVE:To promote safe,effective,economical and proper medication.METHODS:Prescriptions in the outpatient and emergency departments of our hospital in2003were randomly selected;the irrational prescriptions were ana?lyzed with regard to drug descriptions,pharmacological knowledge and literatures.RESULTS:The irrational one amounted to4.92%of the total selected prescriptions;antibiotics drug abuses amounted to84.12%of the irrational ones.The irrational drug use problems were mainly seen in administration scheme,repeated dosage,menstruum,administration,drug combina?tion,compatibility and combined toxicity.CONCLUSIONS:Clinical pharmacist plays an important role in promoting rational drug use by checking prescriptions.

A COMPARISON OF ENZYMATIC ACTIVITIES, Ca~(2+) BINDING AND Ca~(2+) TRANSPORT FUNCTIONS IN SUBCELLULAR MEMBRANE FRACTIONS ISOLATED FROM DOG MESENTERIC ARTERIES AND VEINS / 中国药理学通报

Using subcellular membrane fractions isolated from smooth muscles of dog mesenteric arteries and veins, we compared their enzymatic activities, Ca2+ bindind and Ca2+ transport. The micro-somal (MIC)and plasma membrane(F2) fractions were similarly enriched 4-5 and 10-12 fold respectively in the plasma membrane marker enzymes over the starting membrane mixture. However, MIC and F2 fractions from venous smooth muscle showed higher Mg2+-ATPase activity, slightly increased Ca2+ binding and reduced Ca2+-transport compared to the corresponding fractions from arteries.