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1.
Mem. Inst. Oswaldo Cruz ; 99(1): 53-55, Feb. 2004. graf
Artículo en Inglés | LILACS | ID: lil-356443

RESUMEN

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r² = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Interferón gamma , Mycobacterium tuberculosis , Linfocitos T , Tuberculosis , Técnicas de Cultivo de Célula , Ensayo de Inmunoadsorción Enzimática , Interferón gamma , Prueba de Tuberculina , Tuberculosis
2.
Mem. Inst. Oswaldo Cruz ; 97(8): 1097-1099, Dec. 15, 2002. tab, graf
Artículo en Inglés | LILACS | ID: lil-326339

RESUMEN

The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease


Asunto(s)
Antígenos Bacterianos , Antígenos CD , Antígenos de Diferenciación de Linfocitos T , Linfocitos T CD4-Positivos , Linfocitos T CD8-positivos , Activación de Linfocitos/inmunología , Mycobacterium tuberculosis , Receptores de Interleucina-2 , Aciltransferasas , Proteínas Bacterianas , Ferritinas , Citometría de Flujo , Tuberculina
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