prevalence of ureaplasma urealyticum and mycoplasma genitalium in patients with prostate cancer in shohada hospital in Tehran, Iran

Prostate cancer [PCa] is an important health problem in the aging male population in the world. It is the third most common cancer in the world. Despite of its importance, relatively little is known about its etiology. Sexually transmitted infections [STI] and urogenital pathogens such as Mycoplasma and Ureaplasma, have been proposed as a risk factor for prostate cancer development. This study aimed at detecting the prevalence of Ureaplasma urealyticum [U. urealyticum] and Mycoplasma genitalium [M. genitalium] in PCa and the controls group with benign prostate hyperplasia [BPH] in Shohada hospital. A total of 124 paraffin-embedded prostate tissues [62 PCa patients and 62 controls with BPH] were included in this study. The subjects'specimens were investigated by the polymerase chain reaction method for the presence of U. urealyticum and M. genitalium DNA. U. urealyticum was detected by standard PCR in 1.61% of the 62 PCa patients and there was no DNA U. urealyticum in the 62 controls with BPH. No M. genitalium was detected by standard PCR in the prostates of 124 paraffin-embedded prostate tissues. According to our results, there is no association between M. genitalium and U.urealyticum with PCa. We recommend further studies using a large sample to determine role of Ureaplasma and Mycoplasma in PCa because understanding the role of infectious agents on PCa might be useful for developing new therapeutic approaches and prevention of PCa

Cytoplasmic and membranous CD24 marker expression has indirect correlation with cAMP/cGMP ratio

CD24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. Our aim was to clarify the correlation between CD24 expression and cAMPxGMP ratio in murine colorectal cancer cell line [CT26] after using cholera toxin. The CT26 cells were cultured in flasks for assaying cAMP and cGMP by ELISA kits; also the cells were cultured in flasks for assaying cytoplasmic and membranous CD24 expression. The Real-Time PCR was done for cDNA that was synthesized from CT26 cells' mRNA. Also, expression CD24 marker of cells was determined by Anti-CD24 antibody and Goat Anti-Rabbit IgG-FITC [flow cytometry]. The cholera toxin increases the cAMPrcGMP ratio and influenced the cytoplasmic and membrane CD24 expression level. Cholera toxin increased cAMP. After increasing cAMP/cGMP ratio, cytoplasmic and membranous CD24 expression showed decline