Associative growth of Yersinia enterocolitica 0:3 with Pseudomonas aeruginosa and Staphylococcus aureus in a diffusion apparatus (Ecologen)

To evaluate associative growth of psychorotrophic P. aeruginosa and S. aureus with Y. enterocolitica 0:3, microorganisms were grow in trypticase soy broth in a diffusion apparatus (Ecologen) and incubated for 9 days at 4 grados C. The effects of culture interactions were determined by growth curves, lag times, generation times, pH changes and acid production. The observed growth curves for Y. enterocolitica 0:3, P. aeruginosa and S. aureus, shows the stimulating effects of Y. enterocolitica 0:3 on growth of psychrotrophic microorganisms in associative culture at 4 grados C, although it was later inhibited by these organisms as they reached the stationary phase, thus proving itself to be a poor competitor with common spoilage microorganism