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1.
Indian J Exp Biol ; 2006 Sep; 44(9): 757-61
Artículo en Inglés | IMSEAR | ID: sea-61529

RESUMEN

A system was developed for in vitro regeneration of Pennisetum glaucum through organogenesis and somatic embryogenesis. Mature embryo and leaf base explants of Pennisetum glaucum (L) Br. cv HH B60 (Poaceae) were cultured on Murashige and Skoog agar medium supplemented with 11.3 microM of 2,4-D for callus induction. Embryogenic calli were induced within eight weeks. Percentage of callus induction and somatic embryogenesis was significantly higher in mature embryo than leaf base explants. Maximum shoot regeneration was obtained via organogenesis on MS medium supplemented with 4.43 microM of BAP and 4.64 microM of kinetin from the calli of both the explants. The frequency of plant regeneration through somatic embryogenesis was comparatively lower than organogenesis. Regeneration frequency was higher in mature embryo explants than leaf base explants. The shoots regenerated via organogenesis were elongated and rooted efficiently on MS medium supplemented with IBA (0.49 microM). The rooted plantlets were hardened and transferred to soil.


Asunto(s)
Medios de Cultivo , Técnicas de Cultivo , Pennisetum/crecimiento & desarrollo , Hojas de la Planta/crecimiento & desarrollo , Brotes de la Planta/crecimiento & desarrollo , Regeneración , Semillas/crecimiento & desarrollo
2.
Indian J Exp Biol ; 2000 Dec; 38(12): 1270-3
Artículo en Inglés | IMSEAR | ID: sea-56618

RESUMEN

In vitro shoot regeneration in Pterocarpus santalinus L. was achieved when detached cotyledons from in vitro germinated seedlings were cultured on MS medium containing NAA (0.1 mg/L), BA (1 mg/L) and kinetin (1 mg/L). The regenerated shoots rooted on 1/4 strength MS medium with IAA (1 mg/L) and the fully developed plantlets were successfully established in the soil.


Asunto(s)
Fabaceae/efectos de los fármacos , India , Reguladores del Crecimiento de las Plantas/farmacología , Raíces de Plantas/efectos de los fármacos , Brotes de la Planta/efectos de los fármacos , Plantas Medicinales , Árboles/efectos de los fármacos
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