RESUMEN
Backgrounds and Objectives: Rapid identification of Vibrio cholera is important in epidemics and bioterrorism wars. On the other hand, the conventional method is time consuming and costly. The aim of this study was to develop a rapid, inexpensive and high sensitive method based on magnetic nanoparticles for trapping bacterial DNA
Materials and Methods: In this study, biotinalated probes were used for trapping Vibrio cholerae DNA. Finally designed primers were used to detect bacteria
Results: According to PCR identified results showed that only Vibrio cholera were identified, and; concluded that the biotinalated probe is specific for bacterium Vibrio choleraecholera
Conclusions: According to the findings of this study, Sensitive and specific detection of the bacterium Vibrio cholerae is assessed and validated with this method efficiently. And in terms of time and at lowest costs are introduced an efficient method
RESUMEN
Background and Objectives: electro spinning is a method to produce the submicron nanofiber polymer in fibers produced by this technique have a unique feature in the medical field due to their high porosity and strength, Chitosan has been used as antimicrobial and healing properties, but because of its high viscosity it is unable to form nanofibers by itself To overcome this problem, the polymers such PEO and PYE are used. Henna extract because of its ability to Boost the growth, antibacterial and healing properties were used to form nanofibers with the above polymers
Material and Methods: the solution of chitosan, polyethylene oxide and henna in ratio ranges of 40/60 up to 10/90 were prepared. Then stabilized on the cell culture medium and then after fibroblast cells were culture on it
Results: no significant difference was detected between nanofibers cell cultured compared to controls. As healing of burn or wound is the growth of fibroblast cell and inhabitation of wound infection. Thus nanofibers could potentially be used for ulcer healing and bacterial growth prevention. Thereby the chitosan and henna is a candidate because of its anti-bacterial and boost growth boosting effect
Conclusion: as a result, Chitosan and henna as a candidate anti-bacterial and healing can be used
RESUMEN
Nanofibers as nano-scaffolds are widely used in tissue engineering. Electrospun is away to produce these fiberes. In this study, the synthesis of chitosan nanofibers / polyethylene oxide [CS / PEO] on a very thin membrane of CS as a basement membrane has been investigated. For this purpose, composite solutions with volume fractions - density of CS and PEO, respectively, with ratios of 100 to zero [pure chitosan], 90 to 10 and 80 to 20 in Acetic Acid 5/0 M were prepared. Interactions of two polymers on each other were studied by Fourier transform infra-red spectroscopy [FTIR]. Nanofiber diameter and size were studied with Scanning Electron Microscope [SEM]. CS memebrane polymer solution on the interval the electric field kv 20-18 cm 15-10 and feed rate ml / h 5/0 was electrospun. Nanofiber diameter and size were studied by Scanning Electron Microscope [SEM]. FTIR study of the composite CS / PEO ratio of 20/80 and 10/90 peak tensile bond hydroxyl [OH] and [C = O] chitosan showed in certain areas. Scanning Electron Microscopic images showed that pure CS could not produce nanofibers on any ratios, and they appeared as a droplet. Whereas, nanofibers were created with ratios 80 to 20 and 90 to 10 of CS / PEO composite both on the aluminum foils and thin membrane of CS. It seems that high viscosity of pure CS prevents nanofibers formation. Whereas, homogeneous nanofibers obtained from the composite of CS / PEO with the ratios 80 to 20, 90 to 10 and pure CS on thin membrane of CS