RESUMEN
Background: Mutations in the coding region of the Chemokine Receptor 5 [CCR5] genes reduce or eliminate CCR5 expression in immune cells and progression of HCV infection. This study aimed to investigate the role of this mutation in HCV infection in Iranian patients in comparison with healthy individuals
Methods: 100 HCV infected patients and 100 healthy individuals were randomly selected. The CCR5-DELTA32 genotypes were determined using specific primers and PCR method
Results: The agarose gel electrophoresis showed a189-bp fragment from wild type for both alleles of CCR5 gene. The CCR5-DELTA32 allele was not found in any HCV infected and healthy subjects
Conclusion: The mutation in CCR5 gene was not detected in any of the two groups; therefore, the role of CCR5 gene expression in immune cells and progression of HCV infection needs to be studied in larger samples in our country
RESUMEN
Diabetes mellitus is associated with distribution of cognitive functioning. Hyperglycemia induced oxidative stress has been proposed as a cause of memory complications of diabetes including cognitive impairment. The aim of this study was to examine total green tea extract [TGTE], a potent free radical scavenger against spatial impairment in Streptozotocin-diabetic rats. Eight weeks after diabetes induction, TGTE was administrated throught drinking water 3 mg/L. The learning and memory behavior was evaluated with Morris water maze task in male rats. Then, for estimation of oxidative stress parameters such as lipid peroxidation [LPO], total antioxidant capacity [FRAP] and total thiol groups in blood were measured. The total green tea extract showed improved cognitive impairment in diabetic groups but these changes weren't significant. There was also significant increase FRAP level and total thiol groups in treated green tea groups vs. control. group. This study demonstrated the effectiveness of TGTE on spatial impairment and oxidative stress induced in diabetes mellitus