RESUMEN
Background: Urinary tract infection may be one of the nosocomial infection among orthopedic trauma patient
Objective: To find out the microbial profile of culture positive urine samples from patients of orthopedics ward
Methodology: Study Design: Cross sectional study. Place and Duration of Study: Study was conducted from 1 January to 31 August 2015 at department of Microbiology, Lahore Medical and Dental College, Lahore, Pakistan. Data was taken from culture positive urine samples received from the department of Orthopedics of Ghurki trust and Teaching Hospital. All samples were inoculated on both blood and MacConkey agar. Total 140 culture positive samples were included in this study. Data was entered and analyzed by using SPSS version 20
Results: Out of 140 culture positive urine samples, 46 [32.85%] were found out to be E.coli. It was followed by 24 [17.1%] each of Candida and Acinetobacter species. Pseudomonas was found in 16 [11.42%] cases. Other pathogens isolated were 10 [7.1%] of Klebsiella species, 12 [8.57%] of Proteus species and 8 [5.71%] of Staph. Aureus
Conclusion: E.Coli was found to be in highest number as a cause of nosocomial UTI followed by Acinetobacter, Candida and Pseudomonas
RESUMEN
Background: Pseudomonas aeruginosa is an important pathogen isolated from various clinical samples. It continues to cause complication in nosocomial infections
Objective: To determine the prevalence and antibiotics resistance pattern of P.aeruginosa isolated from a clinical samples at a tertiary care hospital
Methodology: This was a cross sectional study. A total of hundred clinical samples comprising urine, pus, blood and wound swabs were collected from ICU and burn unit, surgical ward, medical ward and OPD of Sheikh Zayed Medical College/Hospital, Rahim Yar Khan. These samples were cultured on MacConkey and Blood agar. Urine was cultured on CLED agar. Plates were aerobically incubated at 35-37 [degree sign]C. Positive cultures were identified by culture characteristics and biochemical reactions. Antimicrobial resistance of all isolated bacteria was done by the disk diffusion testing. Pure culture was obtained then inoculated on Nutrient agar plate using disks of amoxicillin: clavulanic acid, ceftazidime, ciprofloxacin, amikacin, imipenem, piperacillin tazobactam and gentacin. After 24 hrs incubation plates were examined to read the inhibition zones
Results: From these samples Pseudomonas aeruginosa was isolated from urine [17%], pus [26%], blood [9%] and wound swab [48%]. Pseudomonas aeruginosa showed resistance against Piperacillin Tazobactam [99%], Amoxicillin Clavulanic Acid 91%, Tigecycline 89%, Amikacin 83% and Ceftazidime 79%
Conclusion: Our Study showed that P. Aeruginosa, is the common microorganisms isolated resistance pattern is against common antibiotics. To cope with antimicrobial resistance against critically ill patients with pseudomonas infections it is necessary to follow firm antibiotic policies and SOPs while implementation of surveillance programmes for MDR bacteria and infection control and prevention procedures are needed