role of smooth-ended ameloblasts in enamel protein degradation in the rat incisor pigmentation

Rats were perfused with glutaraldehyde. The lower incisors were dissected free. Mid-sagittal slice was cut through the entire incisor, deimneralized. The pigmentation zone was isolated and further sliced into cross sections. These sections were incubated for CMPase. CMPase reaction product were associated with the lateral cell memberane of subclass II and Subclass III. This localization confirm the role of extracellular space of SM as a route of enamel proteins during maturation