RESUMEN
The present study was designed to compare the amount of resistin hormone released by explants of human adipose tissue in primary culture either from the visceral fat compartment or from posterior subcutaneous abdominal adipose tissue mass [PSAATM]. Visceral omental and abdominal subcutaneous adipose tissues, representing group I and II respectively, were obtained from 30 women undergoing abdominoplasty with concomitant procedures necessitating opening of the peritoneal cavity. Isolation of fat cells from adipose tissue was done according to the method of Fain et al At the end of the isolation, each group was further subdivided into 4 subgroups [a-d] according to the separation method which were [a] represented explants of adipose tissue, [b] represented adipose tissue debris remaining after digestion of minced adipose tissue, [c] represented non-fat [Stromal-Vascular [SV]] cells, and [d] represented fat cells. Resistin was assayed in each of the 8 subgroups at 24 hours and 48 hours. The release of resistin over 24 h was about half that seen at 48 h by all subgroups. There was a significant greater release of resistin by explants of adipose tissue, adipose tissue debris, SV cells, and fat cells subgroups of omental adipose tissue when compared to the subgroups of subcutaneous adipose tissue explants. It could be concluded that resistin is made and released at much higher levels by the omental adipose tissues than subcutaneous adipose tissues. Whether or not resistin is produced by: the fat cells or non-fat cells of human adipose tissue, needs further investigations