Studies on infectious bronchitis in broiler chickens in EL Menia Governorate

A total of 986 serum samples were collected from IB non vaccinated 26 broiler farms in El- Menia; A total population of 135500 bird. All tested farms were ELISA positive for IBV antibodies. The percent of positivity were 42.86, 33.04, 48.37, 31.01, 43.09, 34.01 and 33.68 in samples from Abo- Korkas, Bani- Mazar, Dear-Mawas, EI-Menia, Malawy, Mtay and Smaloot; respectively. IB virus was successfully isolated by 4 blind passages in SPF chicken embryos, which showed curling and dwarfing and were positive to Dot-ELISA against reference antibodies IBV. Pathogenicity of the obtained virus isolate was studied in both broiler and layer chicken. In broiler chick, pathogenicity was performed in groups aged 1, 7, and 14 days. No mortality was seen in group infected at 14 day of age, while it was 8/2 chicks in both 1 and 7 days. Examined tissue sections of trachea, lung and kidney of birds at 4 and 14 days post infection in different age groups revealed severe histopathological changes especially in birds inoculated at 1 and 7 days old as compared with birds inoculated at 14 day old. In layer chicken the isolate resulted in mild respiratory manifestations [sneezing, coughing], decline of egg quantity and quality [rough shell and shell less eggs] 2 weeks after challenge as well as mortality reached 20%. Postmortem examination of succumbed broiler and layer birds revealed severe renal congestion and mild congestion in trachea and larynx

Immunological and biochemical effects of nigella sativa and ultra natural plus on turkeys vaccinated with newcastle and turkey rhinotracheitis virus vaccines

The experiment was designed to study the immunological and biochemical effects of Nigella Sativa [NS] and Ultra-Natural plus [UP] on turkey poults vaccinated against Newcastle disease [ND] and Turkey Rhinotracheitis [TRT] virus vaccines. The results indicated the immunostimulant effect of both NS and UP but the former was more superior. Results at 4 weeks of age proved that Nigella Sativa gives ND HIGM titer, Optical density for TRT, Total serum protein and gamma globulin were 8.6, 0.943, 2.88 and 1.02 versus of 7.4, 0.922, 2.55 and 0.85 in Ultra- Natural plus; respectively. Nigella Sativa challenged groups using NDV and TRTv showed 93.34% and 100% respectively while ultra natural plus challenged groups should 86.67% and 93.34%, respectively