RESUMEN
@#BACKGROUND: According to WHO, Non-communicable disease take place 43% of morbidity as well as 63% of mortality in worldwide and it will may increased as 60% of morbidity, 73% of mortality in 2020. Therefore one quarter of NCD associated with composition and usage of foods. Obesity and Diabetes mellitus are caused by accumulating saturated fat in body due to excessive use of high calorie. High-fat diet promotes lipid deposition in solid organs including skeletal muscle which is underlying cause of insulin resistance. METHODS: Same aged, Inbred (C57BL/6J) 20 male mice are involved in this study. They are raised and fed in central core laboratory of MNUMS. Feeding of mice “Beijing Branch of the Joint Feed Co LTD” from Republic of China produces forage for laboratory animals. They were divided into two groups. A control group mice had been fed standard chow while experimental group mice had been fed 45% high fat diet for 4 weeks. The morphological effect of the a high fat diet was examined by light microscopy. RESULTS: Liver of experimental mice was seen pale and parenchyma had a fatty changes compared to control group liver in gross anatomy. Periportal necrosis and lipid droplet deposition in hepatocytes of study group were detected as microscopically. There is fatty accumulation between solid tissue of pancreas and islets of Langerhans get smaller in study group. Experimental mice which were fed by 45% of fat containing food had lipid droplets were observed in fibers. Therefore degenerated fibers showing separation from the basement membrane and acidophilic staining were observed in skeletal muscle.