Cytotoxicity of two species of Glaucium from Iran

Numerous molecules in Papaveraceae family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. Numerous molecules in this family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. In this study we evaluated the cytotoxic effects of total and alkaloid extracts of Glaucium flavum Crantz and Glaucium grandiflurom Boiss. andamp; Huet, the two species of this genus, on cell proliferation of HT-29, Caco-2, T47D, and NIH/3T3 cell lines by MTT method and their IC[50]s were determined. The aerial parts of G. grandiflurom and G. flavum were collected from Jajrud in Tehran Province in June 2011. The effect of total extract and alkaloid extract of then on HT-29, Ta7D, NIH/3T3 and Caco-2 cells was determined by MTT assay. Alkaloid extracts showed a moderate cytotoxic effect on the cell lines. IC[50] values confirmed that the growth and proliferation of NIH/3T3 cells were less affected in comparison to other cell lines. The effects of alkaloid extracts of both plants on human colon adenocarcinoma cell lines [HT-29, Caco-2], showed that these extracts contain certain compounds that can inhibit the proliferation of colon cancerous cells

Anti-inflammatory effect of thymus kotschyanus boiss. and hohen. extract on rat's hind paw edema induced by carrageenan

Advantages of herbal drugs have been known to human being for many years and they have been used for treatment of many diseases. Nowadays, inflammation is known to be one of the basic pathologic causes of the diseases. In this study the anti-inflammatory effects of Thymus kotschyanus Boiss and Hohen was investigated. Forty two Wistar rats were used into three divided groups. In the first group, extract of Thymus kotschyanus Boiss and Hohen in doses of 50,100,150,200 mg/kg was injected intra peritoneal and after one hour they were treated with hind paw edema test with carrageenan. In the second group, Indomethacin with the dose of 5 mg/kg was injected one hour before carrageenan and in the third one, saline was injected before carrageenan, every hours after carrageenan injection to rat's right hind paw; the inflammation was separately measured by the change in the volume of mercury [plethysmometer]. Indomethacin and Thymus kotschyanus Boiss and Hohen extract had significantly reduced edema in all five measurements [p<0.001]. There was no significant difference between Indometacin and the extract in reducing edema in the first to fourth hour, but in the fifth hour, the herbal drug showed a significant difference of anti-inflammatory effect with Indometacin in all doses [p<0.05]. The methanolic extract of Thymus kotschyanus Boiss and Hohen has anti-inflammatory effect in all doses which is dose dependant and can be used instead of or as a synergist for Indometacin. Higher doses of herbal extract may have more anti-inflammatory effects