RESUMEN
Objective: Comparing cytological findings of the uterus and cervix mucusae in two methods [swab and aspiration] in cows during oestrus cycle
Design: Comparative descriptive survey
Animals: A total of 120 cows
Procedure:A total of 120 genital systems of slauthered cows were selected. All the genital systems were contained uterine horns and cervix. In according to the physical appearances, genital samples were divided into estrus, metestrus, diestrus, proestrus, anestrus, post parturition and ovarian cysts. Genital smears were prepaired from uterine horns and cervix by swab and aspiration methods. The smears were stained with Giemsa stain and examined by a microscope
Statistical analysis: The data were analysed statistically using one way analysis of variance [ANOVA]. The differenc between the means were statistically estimated by Duncan's test
Results: There were no significant differences in the percentage of large vacuolated epithelial cells, neutrophils, macrophages and lymphocytes of cervical and uterine mucusae by swab and aspiration methods[P>0/05]. There was a significant difference in the percentage of epithelial cells in the smears obtained from cervix and uterus in swab and aspiration methods. So that the percentage of epithelial cells in swab method were more than aspiration methods [P<0.05]. Comparison of the cervix and uterus cells in swab and aspiration methods in the estrus cycle showed that the average number of neutrophils in estrus were lower than other stages. The average percentage of neutrophils in other stages of estrus were lower than 5%. In the cases with ovarian cyst and postpartum period, the average percentages of neutrophils were more than 5% which can be a sign of inflammatory reaction
Conclusion: With respect to cytological studies, there were no significant differences between the samples which had been taken of the uterine horns and uterine cervix. For uterine cytology, aspiration method proved to be better than swab method