1.
Indian J Exp Biol
; 1998 Dec; 36(12): 1209-15
Artículo
en Inglés
| IMSEAR
| ID: sea-60199
RESUMEN
Transgenic mice were produced to study the expression of amino-3' glycosyl phosphotransferase gene (neomycin resistance gene) in the embryonic fibroblast cells. A 1.9 Kb linear fragment of neomycin resistance gene under the control of pPGK promoter was microinjected into the pronucleus of mouse embryos. Out of 64 potential founders born, 5 were identified to be transgenic by the polymerase chain reaction (PCR) and southern hybridization. Multiple mice from first and second generation from two transgenic founders (N-10 and N-32) were analysed to determine the germline transmission. It was found to be 24.6 and 71.4% in first and second generation respectively. Results were also further confirmed by RT-PCR, sequencing and in vitro bioassays.
Asunto(s)
Animales , Secuencia de Bases , Cartilla de ADN , Farmacorresistencia Microbiana/genética , Femenino , Células Germinativas , Kanamicina Quinasa/genética , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Ratones Transgénicos , Neomicina/farmacología
2.
Artículo
en Inglés
| IMSEAR
| ID: sea-112413