RESUMEN
Tetracycline (TC) antibiotics continue to play an important role in human and veterinary medicine and in animal nutrition. Comparison of three techniques, High Performance thin-layer chromatographic (HPTLC), HPLC and LC-MSMS for the determination of tetracycline has been carried out here. The purpose of this study was to determination of minimum responce tetracyclines and their metabolite by chromatographic techniqes. A rapid and easy‐to‐use method was developed on the liquid chromatography.
RESUMEN
To study oil contents and fatty acid composition among the samples of Jatropha curcas L. seeds were collected from Dehradun, Uttarakhand. Soxhlet extraction method and gas chromatography (GC) were employed to determine the oil contents of Jatropha seeds and the fatty acid composition of Jatropha oil. The seed oil contents (dry basis) was 46.27% .Physicochemical properties shows acid value (36.46), iodine value (106.00 mg/g) and saponification value (194.70 mg/g.The evaluation of fatty acid composition using gas chromatography (GC) revealed that, oleic (44.93%), linoleic acid (33.40 %), Palmitic acid (15.39%) and Stearic acid (6.26%).