RESUMEN
Background: Dengue is believed to infect 50 to 100 million people worldwide in a year. Dengue is a major challenge to public health, especially in South-East Asia. The present study assessed microbiological profile of Dengue fever among patients. Methods: This study was conducted on 120 patients found serologically positive for dengue fever. Rapid immune chromatography method was used for detection of NS1 antigen, and IgM antibody. Results: Common clinical manifestation were headache (20%), vomiting (44%), headache (20%), abdominal pain (19%), myalgia (7%),hepatomegaly (42%) and bleeding (12%). The difference was significant (P<0.05). 85% found positive for NS1 and IgM (7%). 8% samples found positive for NS1 and IgM. The difference was significant (P<0.05). 13 patients developed complications of dengue shock syndrome and 7 developed dengue hemorrhagic syndrome. Conclusion: Diagnosis of dengue fever in children is challenging. Dengue fever can be diagnosed with different laboratory tests. Among all is detection of NS1 antigen which may be considered as early diagnostic marker for dengue fever.
RESUMEN
Background: Candida is the shortened name used to describe a class of fungi that includes more than 150 species of yeast. The present study was conducted to assess Candidiasis infections among patients. Methods: The present study was conducted in the department of Microbiology on 114 cases. Clinical examination was done for the diagnosis. Microbiological culture assessment was done in all cases. Swab was taken from the lesion of the patients and was incubated in Sabouraud’s dextrose agar medium for assessment of culture growth characteristics. All the samples were incubated in the culture medium at 37°C for 1–2 days. Counting of the yeast colonies was done 48 hours after incubation. Results: Age group 11-20 years had 12 males and 7 females, 21-30 years had 18 males and 16 females, 31-40 years had 24 males and 15 females and 41-50 years had 10 males and 12 females. Most common type was denture induced candidiasis (DIC) seen in 20 males and 14 females, acute atrophic candidiasis (AAC) seen in 18 males and 15 females, acute pseudomembranous candidiasis (APC) seen in 12 males and 10 females, chronic hyperplastic candidiasis (CHC) in 10 males and 8 females and angular cheilitis in 4 males and 3 females. The difference was significant (P< 0.05). Common species was C. albicans (52), C. Krusei (32), C. tropicalis (20) and C. pseudotropicalis (10). Conclusion: Candidiasis is a common opportunistic infection. Most cases were seen in males and most common type identified was Candida Albicans.