RESUMEN
Infertility is one of the common problems seen in couples of reproductive age. Presence of antisperm antibodies in semen and serum are amongst the causes of immunoinfertility. This study was performed to determine antisperm antibodies in cervicovaginal secretions and serum of infertile women and also measure serum levels of immunoglobulins [IgG, IgM and IgA]. The study consisted of 45 infertile women consulting the Kammal ElSammrari Hospital for infertility from 2008 to 2009 and the control group consisted of 30 fertile women. Serum levels of immunoglobulins [IgG, IgA and IgM] were measured in the participants using single radial immune diffusion. Antisperm antibodies [ASAs] were detected in the serum of both infertile and control groups using indirect immune fluorescence test. ASAs were also detected in cervicovaginal secretion using direct sperm agglutination test in both infertile and control groups. Antisperm antibodies were found in the cervicovaginal secretions [62.2%] and sera [64.4%] of infertile women which were significantly higher [p <0.001] than those of the control group [3.3% and 3.3% respectively]. There was a significant increase [p <0.001] in serum levels of IgG and IgA of infertile women [16.2 and 3.25 g/L respectively] compared with the healthy control group [7 and 1.2 g/L]. Humoral immune response and antisperm antibodies may contribute to reproductive failure in couples of reproductive age
RESUMEN
Trichomonas tenax [T tenax] is a protozoan that inhabit the oral cavity of poor oral hygiene. It is found in the dental caries, tartar and periodontal area. It feed on the normal flora of the mouth. It had been found to cause pulmonary infection and respiratory diseases in immunocompromised patients and patients with advanced cancer. To determine the humoral immune response to Trichomonas tenax and studying if there is any relation with specific bacteria. Forty patients who consult Al-Kindy Teaching hospital -maxillofacial and dental department from June-2008 to January -2009.Two gingival swabs were taken from those patients, one examined directly for the presence of T tenax by light microscope and other swab was cultured on Blood, Chocolate and MacConkey's agar for isolation of oral bacteria. Blood was collected from patients for estimation of serum IgG, IgA, IgM, C3 and C4 levels by using radial immune diffusion method. The study group consists of forty individuals, their ages range from 6-65 years. Male more than female [22:18], thirty of them was smoker, 25% of them were positive for T tenax. The types of bacteria that were isolated are a normal flora of the mouth like Streptococcus viridans. In spite of the level of Immunoglobulins and complement in both groups lie within normal values, there was a significant increased in serum IgM level and significant decreased in serum IgG, IgA, C3 and C4 level. There was increased in the prevalence of T tenax infection due to low social class and low oral hygiene .There was no specific bacteria that was confected with it. Lastly, increased in T tenax infection when there is an immune suppression as in advanced cancer patients and on radiotherapy and or chemotherapy