RESUMEN
Many associations have been found between specific HL.4 antigens and increased susceptibility to various diseases. So we tried to associate class I and class II antigens with acute lymphoblastic leukemia. We also demonstrate the presence of antibodies in serum of acute lymphoblastic leukemia patients against HLA class I. Prospective study. Tissue typing and histocompatibility center at .Al-Karamah Teaching Hospital. 70 acute Lymphoblastic leukemia patients from pediatric hospitals. HLA [human leukocyte antigens [typing done for them by serological method and cross matching and blood grouping were also done for them. there was significant difference between patients and control groups regarding HLA -C6, D R1, DR4, DR7, DQI, DQ2, DQ3, DQ4. There was14.2% [1 0/70] of patients had antibodies against HLA class I. There were two significant associations between blood group and acute lymphoblastic leukemia. Genetic factor increased susceptibility with acute lymphoblastic leukemia HLA- DRI DQI, HLA - DR4 DQ4, HLA- DR4 DQ3, and HLA- DR7 DQ2. This HLA typing increased susceptibility to be affected with leukemia after infection. HLA typing was done to acute lymphoblastic leukemia patients by molecular -DNA based method [PCR-SSP, RSC4] in addition to serological method
RESUMEN
Iraq had more than twenty-four millions inhabitants of populations. This nation is one of the most populated countries in the world.it is difficult to define Iraqi populations genetically [HLA polymorphism] because they are structured of a mixture of many groups. [HLA phenotype frequencies that encoded by many closely. linked genes that are responsible for a variety of cell surface alloantigen proteins that are responsible for differences in different ethnic groups. .Arab Christians in Iraq. accounting for more than .three millions inhabitant mostly the northwest and other yurts of Iraq. This raised the need for a preliminary study of the HLA trend in this population. 1- Estimating the gene frequency of HLA class I [A. B. C w] and class 11 [DR and DQ] alleles in Iraqi Arab Christians. 2-Assessing the genetic relationship between Iraqi .Arab Christians and other Arabian, Asian and European populations. A total of underrated 568 Iraqi Arab Christians [AC] healthy volunteers and individuals referred to Immunology and. Tissue Typing Center in Al Karamah Teaching Hospital for organ transplantation and Forensic medicine were examined for HLA polymorphism using complement dependent cytotoxicity test from June-2003 to .April-2004 The phenotypes of all loci [AC]] were in agreement with Hardy-Weinberg equilibrium In case of. HLA-A locus. three variants dominate this locus A1 [0.123], A2 /[0.149] and .A3[0.134] which showed some similarities with Arabian .Asian and European people in the world. A3 had been found to be associated with Hereditary Hemochromatosis HHI but the risk increased when there is linkage disequilibrium between A3 B14. Fortunately B14 allele had a low frequency. In this group and common allele was B35 [0.154], and B5 [0.128] which had an association with Behcet's syndrome. So one can predict high incidence of this disease in this group of populntioin. Last locus was studied in class I was Cw4 that had a higher rate [0.173] in HLA Cw loci. In case of class II, it was done on small number of persons and the common allele was DR2 [53.84%] which is protective from insulin dependent diabetes mellitus disease while DQ1 is common allele in HLA-DQ loci. HLA typing of [AC] had some similarity with Arabian people because of their same ancestry and also had some similarly with Caucasoid Europeans because of outbreeding and intermixing with those populations due to migration