RESUMEN
<p><b>OBJECTIVE</b>To study the effects of E2F-1-silencing lentivirus vector on the growth and chemoresistance of subcutaneous human gastric cancer in nude mice.</p><p><b>METHODS</b>Thirty-six nude mice were inoculated subcutaneously with chemoresistant SGC-7901/DDP cells to establish subcutaneous tumor models of gastric carcinoma. The mice were randomly divided into E2F-1/RNAi-LV group, LV-scrRNAi group and PBS group (n = 12). E2F-1/RNAi-LV, LV-scrRNAi or PBS (0.1 ml per time) was injected into the mice, respectively, every two days. The nude mice received an intraperitoneal injection of cisplatin (25 mg/kg) every two days. The tumor volume was measured and histopathological changes of the tumors were observed by HE staining. The expressions of E2F-1, c-Myc, survivin, MDR1 and MRP were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Apoptosis in tumor xenografts was determined by in situ TUNEL labeling technique.</p><p><b>RESULTS</b>The mean tumor growth rate of the E2F-1/RNAi-LV group was significantly slower than that of the LV-scrRNAi and control groups (P < 0.05). The tumor volume of the E2F-1/RNAi-LV group was (745.13 ± 154.42)mm(3), significantly lower than that of the LV-scrRNAi and PBS groups (P < 0.05). Compared with that in the LV-scrRNAi and PBS groups, the expressions of mRNA and protein of E2F-1, c-Myc, survivin, MDR1 and MRP were significantly decreased in the E2F-1/RNAi-LV group (P < 0.05). The apoptotic rate in the E2F-1/RNAi-LV treatment group was (27.5 ± 9.7)%, significantly higher than (7.0 ± 1.1)% in the LV-scrRNAi group and (7.3 ± 1.2)% in the PBS group (P < 0.05).</p><p><b>CONCLUSION</b>Intra-tumoral injection of E2F-1/RNAi-LV shows significantly inhibitory effect on the tumor growth and chemoresistance of subcutaneous human gastric cancer in nude mice.</p>
Asunto(s)
Animales , Femenino , Humanos , Ratones , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP , Genética , Metabolismo , Antineoplásicos , Farmacología , Apoptosis , Línea Celular Tumoral , Cisplatino , Farmacología , Resistencia a Antineoplásicos , Factor de Transcripción E2F1 , Genética , Metabolismo , Silenciador del Gen , Vectores Genéticos , Proteínas Inhibidoras de la Apoptosis , Genética , Metabolismo , Lentivirus , Genética , Ratones Desnudos , Proteínas Asociadas a Resistencia a Múltiples Medicamentos , Genética , Metabolismo , Trasplante de Neoplasias , Proteínas Proto-Oncogénicas c-myc , Genética , Metabolismo , ARN Mensajero , Metabolismo , Distribución Aleatoria , Proteínas Represoras , Genética , Metabolismo , Neoplasias Gástricas , Genética , Metabolismo , Patología , Transfección , Carga TumoralRESUMEN
Objective To investigate and evaluate the pathological features and diagnostic significance of MSCT findings in scan postprocessing for focal nodular hyperplasia (FNH).Methods A total of 24 patients with FNH who underwent MSCT scan post processing were investigated.The FNH were pathologically and clinically confirmed.Results There are single focus in 22 cases and multiple focus in 2 cases.On plane scan 16 lesions were hypodensity and other 7 isodensity,4 hyperdensity( cases with fatty liver).After contrast,17 lesions were evenly high enhanced on arterial phase;13 lesions showed still hyperdensity and other 10 lesions isodensity,4 lesions hypodensity on parenchymatous phase.Only 5 lesions were hyperdensity but 9 lesions isodensity and 13 lesions hypodensity on delayed phase.Twelve lesions in 27 were detected with central stella-formed cicatrix.There were thickening tumor feeding arteries in 24 lesions,the artery of which were entered integrated into parenchymatous lesions with soft course.Conclusion Synthesizing the charcteristic appearance on MSCT scan postprocessing,doctor could make correct diagnosis and differentiated diagnosis for FNH.