Effects of components in stasis-resolving and collateral-dredging Chinese herbal medicines on angiogenesis and inflammatory response of human umbilical vein endothelial cells induced by VEGF / 中国中药杂志

The present study investigated the mechanism of components in stasis-resolving and collateral-dredging Chinese herbal medicines, including scutellarin(Scu), paeonol(Pae), and hydroxy safflower yellow A(HSYA), in the treatment of psoriasis by regulating angiogenesis and inflammation. The human umbilical vein endothelial cells(HUVECs) cultured in vitro were divided into a normal group, a model group, a VEGFR tyrosine kinase inhibitor Ⅱ(VRI) group, and Scu, Pae, and HSYA groups with low, me-dium, and high doses. Cell viability was detected by the CCK-8 assay. Cell migration was detected by wound healing assay. Tube formation assay was used to measure the tube formation ability. Western blot was used to detect the protein expression of the VEGFR2/Akt/ERK1/2 signaling pathway. The secretion levels of inflammatory cytokines IFN-γ, IL-1β, IL-6, and TNF-α were detected by ELISA. The results showed that compared with the model group, all the Scu, Pae, and HSYA groups could reduce cell viability, inhibit cell migration and tube formation(P<0.05, P<0.01), and down-regulated the protein expression of VEGFR2, p-VEGFR2, Akt, p-Akt, ERK1/2, and p-ERK1/2. Scu and Pae could down-regulate VEGFR2 expression(P<0.05, P<0.01), while other groups only showed a downward trend. Scu and Pae significantly reduced IFN-γ and IL-6 levels(P<0.01), and HSYA significantly reduced the levels of IFN-γ, IL-1β, and IL-6(P<0.01). Scu, Pae, and HSYA had no significant effect on TNF-α. The results suggested that Scu, Pae, and HSYA may exert a therapeutic role in psoriasis-related angiogenesis and inflammation by inhibiting VEGFR2/Akt/ERK1/2 signaling pathway and inhibiting the secretion of IFN-γ, IL-1β, and IL-6.

A meta-analysis of efficacy and safety of compound glycyrrhizin combined with tripterygium wilfordii polyglycosides in treating psoriasis / 国际中医中药杂志

Objective:To evaluate the clinical efficacy and safety of compound glycyrrhizin combined with tripterygium wilfordii polyglycosides for psoriasis.Methods:Literature search was conducted in CNKI, Wanfang, VIP databases, Sinomed, PubMed, the Cochrane Library and Embase with the deadline of September 28th, 2020. The randomly controlled trials of compound glycyrrhizin combined with tripterygium wilfordii polyglycosides in treating psoriasis was included. The publication bias and methodology quality of RCTs were evaluated according to the Cochrane 5.0.1 bias risk assessment method. The RevMan 5.3 software was used for meta analysis.Results:Ten studies with 961 patients were included, with the experimental group ( n=486) and the control group ( n=475). The results of meta-analysis showed that the total effective rate [ OR=3.16, 95% CI (2.00-4.99), P<0.001], the cure rate [ OR=2.56, 95% CI (1.94-3.61), P<0.001], the recurrence rate [ OR=0.15, 95% CI (0.04-0.60), P=0.007], the total incidence of adverse reactions [ OR=0.53, 95% CI (0.34-0.82), P=0.004], the incidence of abnormal liver function [ OR=0.17, 95% CI (0.06-0.47), P=0.001] in the experimental group were better than those in the control group. There was no significant difference on the incidence of adverse reactions of digestive tract ( P>0.05) or lower extremity or facial edema ( P>0.05) between both groups. Conclusions:Compound glycyrrhizin combined with tripterygium wilfordii polyglycosides is effective in the treatment of psoriasis than tripterygium wilfordii polyglycosides alone, and can reduce the recurrence rate and the incidence of adverse reactions. However, a large number of large samples and high-quality studies are still needed.

Influence of small and dense low density lipoprotein on in-stent restenosis after PCI / 心血管康复医学杂志

Objective :To explore influence of small and dense low density lipoprotein (sdLDL) on in-stent restenosis after percutaneous coronary intervention (PCI).Methods : A total of 631 CHD inpatients ,who received PCI in our department from Feb 2015 to Feb 2017were followed up for 12 months ,and 240 cases were successfully followed up . According to coronary angiography (CAG) results ,the vascular diameter stenosis ≥50% regard as in-stent resteno-sis ,all patients were divided into restenosis group (n＝105) and no stenosis group (n＝135).Another 35 healthy volunteers undergoing physical examination simultaneously were treated as healthy control groups .Serum levels of sdLDL ,serum lipid TC ,TG ,HDL-C and LDL-C were measured and compared among all groups .Correlation be-tween serum sdLDL level and Gensini score was analyzed in CHD patients .Results : There were no significant difference in levels of serumlipids between restenosis group and no stenosis group , P＞ 0. 05 all.Compared with healthy control group ,there was significant rise in serum sdLDL level [ (0.451 ± 0.135) mmol/Lvs .(0.673 ± 0.281) mmol/L] in CHD group , P＝0.001 ;compared with no stenosis group ,there were significant rise in serum sdLDL level [(0.606 ± 0.276) mmol/L vs.(0.695 ± 0.304) mmol/L] and Gensini score [(40.23 ± 9.24) scores vs. (58.42 ± 10.37) scores] in restenosis group (P＝0.019 ,0.001) respectively.Linear correlation analysis indicated that serum sdLDL level was significant positively correlated with Gensini score in CHD patients ( r＝ 0.514 , P＝ 0.032).Conclusion :Elevated serum sdLDL level suggests high risk of in-stent restenosis in CHD patients .It can provide reference for disease condition assessment and adverse event prevention after PCI .

The measurement of proportion and function of regulatory T cells in unexplained recurrent spontaneous abortion / 中华妇产科杂志

Objective To investigate the proportion and function of CD_4~+ CD_(25)~+ regulatory T cells (CD_4~+ CD_(25)~+ Tr)in unexplained recurrent spontaneous abortion(URSA).Methods(1)Proportion measurement:the proportion of CD_4~+ CD_(25)~+ Tr cells in peripheral blood was measured by double-label flow cytometric analysis.The samples were taken from 15 URSA women,15 normal non-pregnancy women and 13 normal pregnancy women.(2)Function measurement:CD_4~+ CD_(25)~+ Tr ceils and CD_4~+ CD_(25)~+ T ce]ls were extracted from peripheral blood lymphocytes by the microbeads separation.The purity of CD_4~+ CD_(25)~+ Tr cells and CD_4~+ CD_(25)~+ T cells was measured by flow cytometry.The growth inhibitory effect of CD_4~+ CD_(25)~+ Tr cells on CD_4~+ CD_(25)~+ T cells was assessed in vitro.Results The proportion of CD_4~+ CD_(25)~+ Tr cells was decreased significantly in URSA women(6.9?1.8)% than that in normal non-pregnancy women[(10.8?1.1)%] (P0.05).Conclusion The results suggest that decrease in proportion and function of CD_4~+ CD_(25)~+ Tr cells may be associated with URSA.