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OBJECTIVE: To establish an RP-HPLC analytical method for simultaneous determination of crysoeriol and centaureidin in the aeries parts of Echinops integrifolius. METHODS: The separation was achieved on a Kromasil C18 column (4.6 mm×250 mm, 5 μm)at 30℃ using acetonitrile-water-acetic acid solution (35∶65∶2) as mobile phase at a flow rate of 1.0 mL·min-1 and 350 nm as the detection wavelength. RESULTS: The linear ranges of crysoeriol and centaureidin were 0.4-2.4 mg·L-1(r=0.999 8), 0.6-2.6 mg·L-1 (r=0.999 9), respectively. The average recoveries (n=6) were 91.6% and 92.7%, and RSDs were 1.37% and 1.08% respectively. CONCLUSION: The methodology validation shows that this method is accurate, simple and reliable, which is applicable for the simultaneous determination of two flavonols crysoeriol and centaureidin in the aeries parts of Echinops integrifolius.
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Obiective To investigate the chemical constituents of Pothos chinensis.Methods Compounds were isolated by the silica gel and Sephadex LH-20,semi-PHPLC,preparative thin layer chromatography and recrystallization.Structures of the com-pounds were identified by the physicochemical properties as well as the 1H NMR,13C NMR and MS spectroscopic data.Results Six-teen compounds were isolated from the whole plant of P.chinensis and their structures were identified as liquiritigenin(1),p-hydroxy-benzoic acid(2),syringic acid(3),E-4-hydroxycinnamic acid(4),N-trans-p-coumaroyltyramine(5),N-trans-cinnamoyltyramine (6),N-trans-feruloyltyramine(7),(+)-epiloliolide(8),neoechinulin A(9),vitexin(10),berchemolide(11),(±)-syringaresinol (12),prunasin(13),4-hydroxy-3,5-dimethoxybenzaldehyde(14),β-sitosterol(15)and β-daucosterol(16),respectively.Conclu-sion Compounds 1 and 9-14 are isolated from the genus Pohos for the first time.
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Asteraceae Echionopos (Echinops genus) is one of the world's plants commonly used in traditional Chinese medicine herbs. In this paper, a lot of literature on the basis of domestic and foreign, and modern research on the application of medicinal plants in different ethnic groups in our country Echionopos made a brief presentation and evaluation, and its in Chinese Han, Mongolian, Uygur, Kazak, Korean and other ethnic groups in the application of traditional medicine and related research conducted profiles. To further comprehensive and accurate use of the abundant plant resources and research, in order to find biologically active natural products, provide reference basis for the clinical application of new drugs developed and expanded drug source.