A Case of Bilateral Facial Nerve Palsy Associated with Infectious Mononucleosis / 대한이비인후과학회지

Infectious mononucleosis, a common systemic illness primarily involving children and young adults, is due apparently to the Epstein-Barr virus (EBV). Although neurologic complications of infectious mononucleosis are rare, these include meningoencephalitis, Guillain-Barre syndrome and mononeuritis, and facial nerve paralysis. Bilateral facial nerve palsy rarely develops in patients with a primary Ebstein-Barr virus (EBV) infection, and only a few of these have clinical manifestations of infectious mononucleosis. We report a case of a 18-year-old man with bilateral facial nerve paralysis associated with infectious mononucleosis.

Endoscopic Dacryocystorhinostomy for the Treatment of Lacrimal Sac Abscess

In the past, the treatments for acute dacryocystitis with abscess formation were warm compression, systemic and topical antibiotics, and drainage of the abscess, if fluctuation occurs. Acute dacryocystitis with abscess formation is rarely primary, but often secondary to distal obstruction or extension of contiguous inflammation. After resolution of acute infection, evaluation and treatment of the underlying cause should be initiated. Recent reports have demonstrated that endoscopic dacryocystorhinostomy is an effective treatment for acute dacryocystitis associated with nasolacrimal duct obstruction. We experienced a case of acute dacryocystitis with lacrimal sac abscess formation. Drainage of lacrimal sac abscess was safely accomplished with endoscopic dacryocystorhinostomy. The patient had an excellent postoperative course without recurrence.

Expression of Bovine Growth Hormone Gene in a Baculovirus, Hyphantria cunea Nuclear Polyhedrosis Virus

Bovine growth hormone (bGH) gene was expressed in an insect spodoptera frugiperda cell line using a Baculovirus, Hyphantria cunea nuclear polyhedrosis virus (HcNPV). The bGH gene in pbGH plasmid was sequenced and amplified by PCR technique with two primers containing NcoI sites. The bGH gene consisted of 654 bp (217 amino acid residues), the 5'-untranslated region of the cloned bGH cDNA contains 56 bp, and the 3'-untranslated region contains 145 bp and two pallindromic regions. The amplified bGH gene DNA fragment (654 bp) was inserted into the NcoI site of the pHcEVII vector, which was named pHcbGH. The pHcbGH transfer vector DNA and the wild type HcNPV DNA were cotransfected into s. frugiperda cells to construct a recombinant virus. Eight recombinant viruses were selected and named HcbGH. One clone, HcbGH-4-1 showed largest plaque size, therefore the recombinant virus was further studied. The multiplication patters of the recombinant HcbGH-4-1 was similar to that of the wild type HcNPV. The bGH gene DNA in the HcbGH-4-1 recombinant was confirmed by Southern lot hybridization. The amount of the bGH (217 amino acid residues, 21 kDa) produced in S. frugiperda cells infected with the HcbGH-4-1 recombinant was approximately 5.5 ng per ml (106 cells) by radioimmunoassay.