Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits

PURPOSE: To establish a model of chronic kidney disease in White New Zealand rabbits, using an exclusive unilateral technique of renal ischemia and reperfusion. METHODS: Twenty males White New Zealand rabbits were used. All animals were subjected to the following: pre-surgical blood collection (1st collection) for creatinine and urea serum analysis, left renal ischemia and reperfusion surgery technique, another blood sample was collected after 6 weeks post surgery (2nd collection), the last blood sample (3rd collection) blood sample was taken 11 weeks post surgery (pre-euthanasia), euthanasia and withdrawal of right and left kidney for histopathological analysis. RESULTS: The creatinine levels after surgery was statistically significant higher in the 3rd collection, regarding the 1st and 2nd collection (p<0.05). A significant statistic increase for urea was showed only in the 2nd collection (p<0.05) when compared to the 1st and 3rd collections. Histopathological analysis showed bilateral lesions in the renal tissue, consistent to the process of ischemia and reperfusion. CONCLUSION: This exclusive unilateral technique of renal ischemia and reperfusion without nephrectomy in White New Zealand rabbits, showed effectiveness in getting an animal model of chronic kidney disease .

Ação dos flavonóides na cicatrização por segunda intenção em feridas limpas induzidas cirurgicamente em ratos Wistar / Flavonoid action in second intention healing in surgically-induced

Os flavonóides representam um dos principais grupos de substâncias com atividades farmacológicas em plantas, e são utilizados para elevar a eficácia do processo de cicatrização em feridas e úlceras. A própolis e o chá verde (Camellia sinensis) são dois produtos ricos em flavonóides que apresentam indicações como antiinflamatórios, antibacterianos, antioxidantes e cicatrizantes. O presente estudo tempor objetivo avaliar a ação da própolis e do chá verde no processo de cicatrização em feridas limpas. As feridas foram produzidas em 20 ratos Wistar, e em cada rato, foram induzidas duas lesões simétricas de 11 mm de diâmetro, utilizando-se um Punch cirúrgico. As lesões se localizavam do lado esquerdo e direito da região torácica lateral. Posteriormente, os ratos foram divididos em 2 grupos. Grupo 1 - grupo tratado com soro fisiológico à 0,9 por cento (lateral esquerda) e com creme a base de chá verde (Camellia sinensis) à 20 por cento (lateral direita); Grupo 2 - grupo tratado com creme base (lateral esquerda) e tratados com creme a base de própolis à 20 por cento (lateral direita). Os diâmetros das lesões foram medidos a cada três dias. A cicatrização das lesões ocorreu em 15 dias, sendo que não houve diferença significativa entre os tratamentos, apesar dos grupos tratados com creme de chá verde e de própolis apresentaram melhor evolução durante o tratamento.

Flavonoids represent one of the main groups of substances with pharmacological activities in plants.They are used to increase the effectiveness of the healing process in wounds and ulcers. Propolis andgreen tea (Camellia sinensis) are two products rich in flavonoids that present indications as: antiinflammatory,antibacterial, antioxidant and healing ones. The present study aims to evaluate the actionof propolis and green tea on the healing process in clean wounds. The wounds were produced in 20Wistar rats. In each rat two symmetrical lesions of 11 mm diameter were induced using a surgical punch.The lesions were located on the left and right sides of the lateral thoracic region. Later the rats weredivided in 2 groups. Group 1 – treated with 0.9 percent physiologic solution (left side), and with green teabasedcream (Camellia sinensis) at 20 percent (right side); Group 2 – treated with base cream (left side) andtreated with propolis-based cream at 20 percent (right side). The lesions diameters were measured every three.