Field trial of the ICT filariasis for diagnosis of Wuchereria bancrofti infections in an endemic population of Thailand.

The ICT Filariasis, a rapid card test format, which is based on qualitative detection by monoclonal antibody of the circulating antigen of Wuchereria bancrofti adult worm, is a new diagnostic test of choice for determining the infections under field conditions. By using clinical and recall techniques and microscopy (thick smear and capillary tube technique) as reference, we assessed the efficiency of the ICT card test in sera from 225 subjects living in W. bancrofti-endemic villages of Tak Province, Thailand, who were recruited during a cross-sectional community survey. The ICT card test gave a 20% antigen positive rate, while other tests gave lower positive rates of the same 5.8% by clinical and recall techniques and thick smear, and 5.3% by capillary tube technique, respectively. The ICT card test had a specificity of 100% when sera from microfilaremic subjects were positive, as when sera from W. bancrofti non-endemic subjects either with Brugia malayi microfilaremia or with other parasites, and those from normal controls were all negative by the test. When done in W. bancrofti microfilaremia sera, the ICT card test had a sensitivity of 100% using a microscopy as reference, and 84.6% when using clinical and recall techniques. However, the ICT card test was more sensitive than the others when done in endemic normal sera (14% positive). Such findings compared well with findings in endemic area of South America, suggested its usefulness to detect W. bancrofti infections in endemic area, especially on the Thai-Myanmar border.

Cervical and vaginal agenesis: a case report.

An 18-year-old single female presented with primary amenorrhea and cyclic lower abdominal pain. Pelvic examination revealed normal appearing external genitalia but absent vagina. Pelvic sonogram was compatible with cervical and vaginal agenesis, which was confirmed at operation. Hysterectomy and construction of a new vagina by the McIndoe's technique were performed. Clinical features and management of this rare Mullerian anomaly were reviewed and discussed.