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OBJECTIVE To investigate the clinical practicability of inducible clindamycin resistance and to evaluate the clinical value of cefoxitin disk diffusion method in detecting the mecA gene of meticillin-resistant Staphylococcus(MRS). METHODS Resistant phenotypes were classified with erythromycin and clindamycin discs using the D test method and the cefoxitin disk diffusion method in detecting mecA gene of the staphylococcus. RESULTS Of all 390 Staphylococcus strains,218 strains(55.9 %) were resistant to erythromycin and clindamycin(cMLS).Seventy strains(17.9 %) which were resistant to erythromycin but susceptible to clindamycin showed the positive D test results(iMLS),79 strains(20.3 %) resistant to erythromycin but susceptible to clindamycin showed the negative D test results(MS).From 390 strains of clinical isolated staphylococcus,which using the cefoxitin disk diffusion method,272 strains were MRS. CONCLUSIONS Using the D test method to investigate the inducible resistance to erythromycin and clindamycin in the Staphylococcus strains,which could help the doctors choose correctly the antibiotics such as macrolides and clindamycin.At the same time,cefoxitin disk diffusion method can be used reliably by the clinical laboratory in detecting and conforming MRS.
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@#目的分析血常规检测中常见的误差来源,便于建立室内质量保证措施,确保结果准确。方法用全自动血球仪对我院226例就诊患者的血常规标本进行集中检测,对其血常规检测中常见的一些问题进行分析,包括方法学、仪器、试剂、标本采集、外部环境以及操作过程等综合分析。结果针对引起结果偏差的原因,制定相应的控制措施,确保每一份标本检测结果在质量控制范围内,减少非疾病因素对血常规检测结果的准确性的影响。结论针对血常规检测过程中经常出现的问题进行分析,加强标本分析前、中、后的质量控制,影响结果的一些因素是可以防范的。