Validation of an HPLC-DAD method for the determination of plant phenolics

Abstract A selective, sensitive and precise reversed phase HPLC-DAD method was developed and validated for the simultaneous determination of six phenolic acids in the aqueous extract and their hydrolyzed forms prepared from Solanum elaeagnifolium Cav., Solanaceae, Ampelocissus acapulcensis (Kunth) Planch., Vitaceae, or Brosimum alicastrum Sw., Moraceae. The new method showed good linearity (r > 0.999) in a relatively wide concentration range (0.5-100 mg/l). The limits of detection and quantification for the compounds were in the range of 0.097-0.467 mg/l and 0.097-0.496 mg/l, respectively. The recoveries of compounds were calculated in three different concentrations in the range of 88.07-109.17% and matrix effect was less than 5% for all phenolic acids. Finally, our developed HPLC method is simple, reliable and successfully applied to identify and quantify the phenolic acids in complex aqueous extracts from medicinal species, that can be useful for the analysis of infusions that people consume in folk medicine.

Sindrome de enmascaramiento en un paciente con un linfoma primario del sistema nervioso central / Masquerade syndrome in a patient with a primary central nervous system lymphoma

Se describe el caso de un varón de 37 años con un linfoma primario del sistema nervioso central y con múltiples localizaciones supra e infratentoriales. El paciente presentaba manifestaciones de inflamación intraocular como expresión de su neoplasia intracraneana (síndrome de enmascaramiento) y linfocitopenia ûcon un recuento disminuido de CD4û como representación de una inmunodeficiencia cuya etiología no logramos identificar. El diagnóstico de linfoma se confirmó a través del estudio citológico del humor vítreo. El paciente falleció10 meses después del comienzo de los síntomas.

Endocarditis infecciosa con absceso perivalvular en un paciente con bacteriemia por Erysipelothrix rhusiopathiae / Infective endocarditis with perivalvular abscess in a patient with Erysipelothrix rhusiopathiae bacteremia

The bacterial species Erysipelothrix rhusiopathiae is found worldwide as a commensal or a pathogen in a variety of animals. One well-defined pattern of human infection is an uncommon bacteremic form, with or without cutaneous involvement, usually complicated by endocarditis. We report the case of a 38-year-old male butcher with E. rhusiopathiae bacteremia, native aortic valve endocarditis and perivalvular abscess. The patient was released after six weeks of intravenous ceftriaxone and aortic valve replacement because of severe regurgitation