The SISBIOTA-Diptera Brazilian Network: A long-term survey of Diptera from unexplored Brazilian Western Arc of Amazon, Cerrado, and Pantanal

ABSTRACT The SISBIOTA-BRASIL was a three-year multimillion-dollar research program of the Brazilian government to document plants and animals in endangered/understudied areas and biomes in Brazil. Distributional patterns and the historical events that generated them are extensively unknown regarding Brazilian fauna and flora. This deficiency hinders the development of conservation policies and the understanding of evolutionary processes. Conservation decisions depend on precise knowledge of the taxonomy and geographic distribution of species. Given such a premise, we proposed to research the diversity of Diptera of the Brazilian western arc of Amazon, Cerrado, and Pantanal in the states of Mato Grosso, Mato Grosso do Sul, and Rondônia. Three important biomes of the South American continent characterize these Brazilian states: Amazon forest, Cerrado (Brazilian Savannah), and Pantanal. Besides their ecological relevance, these biomes historically lack intensive entomological surveys. Therefore, they are much underrepresented in the Brazilian natural history collections and in the scientific literature, which is further aggravated by the fact that these areas are being exponentially and rapidly converted to commercial lands. Our project involved over 90 collaborators from 24 different Brazilian institutions and one from Colombia among researchers, postdocs, graduate and undergraduate students, and technicians. We processed and analyzed nearly 300,000 specimens from ~60 families of Diptera collected with a large variety of methods in the sampled areas. Here, we provide a detailed overview of the genera and species diversity of 41 families treated. Our results point to a total of 2,130 species and 514 genera compiled and identified for the three states altogether, with an increase of 41% and 29% in the numbers of species and genera known for the three states combined, respectively. Overall, the 10 most species-rich families were Tachinidae, Cecidomyiidae, Tabanidae, Psychodidae, Sarcophagidae, Stratiomyidae, Bombyliidae, Syrphidae, Tephritidae, and Asilidae. The 10 most diverse in the number of genera were Tachinidae, Stratiomyidae, Asilidae, Mycetophilidae, Syrphidae, Tabanidae, Muscidae, Dolichopodidae, Sarcophagidae, and Chloropidae. So far, 111 scientific papers were published regarding taxonomic, phylogenetic, and biogeographical aspects of the studied families, with the description of 101 new species and three new genera. We expect that additional publications will result from this investigation because several specimens are now curated and being researched by specialists.

Pseudoaneurisma de artéria ulnar superficial / Superficial ulnar artery pseudoaneurysm

Resumo A artéria ulnar é o maior ramo terminal da artéria braquial, ela apresenta origem na fossa cubital e é coberta pelos músculos flexores do antebraço. Reportamos uma variação anatômica, na qual a artéria ulnar situava-se em posição superficial no antebraço. Por falta de conhecimento sobre essa variação, ocorreu a lesão após uma tentativa de punção venosa, a qual levou à formação de um pseudoaneurisma.

Abstract The ulnar artery is the larger terminal branch of the brachial artery. It originates in the cubital fossa and is covered by the flexor muscles of the forearm. We report an anatomic variant in which the ulnar artery was in a superficial position in the forearm. Since this variant was unknown, an attempted venous puncture injured the artery, causing formation of a pseudoaneurysm.

Antimicrobial action of psidium guajava l. extract against enterococcus faecalis and candida albicans strains: a pilot study / Ação antimicrobiana do extrato de psidium guajava l. contra as cepas de enterococcus faecalis e candida albicans: um estudo piloto / Acción antimicrobiana de la extracción de psidium guajava l. contra los cepes de los enterococcus faecalis y los candida albicans: un estudio piloto

Endodontic treatment consists of decontamination of the root canal using irrigants like Sodium Hypochlorite (NaOCl). However, some microorganisms resist conventional therapies, causing relapse and secondary infections. Substances with microbicidal power and potential to become new irrigants need to be studied. Thus, the objective of the present study was to evaluate the antimicrobial potential of Psidium guajava L. (guava tree) extract against clinical and standard (ATCC) strains of Enterococcus faecalis and Candida albicans. For this purpose, the extract was produced from the raw material (leaves and shoots) diluted in the hydroethanolic vehicle (EtOH: H2O / 50:50) for subsequent microbiological analysis. The Minimum Inhibitory Concentration (MIC) and Minimum Microbicidal Concentration (MMC) of the plant extract were tested, according to the Clinical and laboratory standards institute (CLSI) guidelines, on four strains: E. faecalis (ATCC and clinic) and C. albicans (ATCC and clinic). The extract of P. guajava did not produce antifungal action on C. albicans, however, it showed microbicidal potential against strains of E. faecalis, showing MIC of 0.20%. This concentration was lower than the MIC of NaOCl which was 0.31%, a solution that is commonly used in dental clinics. In conclusion, the hydroethanolic extract of P. guajava presents bactericidal action against E. faecalis, being a natural product with potential for future studies regarding the development of new endodontic irrigants.

O tratamento endodôntico consiste na descontaminação do canal radicular com emprego de soluções irrigadoras, como por exemplo, o Hipoclorito de Sódio (NaOCl). Contudo, alguns microrganismos resistem as terapias convencionais, causando recidiva e infecções secundárias. Substâncias com poder microbicida e potencial para se tornarem novas soluções irrigadoras precisam ser estudadas. Assim, o objetivo do presente estudo foi avaliar o potencial antimicrobiano do extrato de Psidium guajava L. (goiabeira) contra cepas clínicas e padrão (ATCC) de Enterococcus faecalis e Candida albicans. Para tanto, o extrato foi produzido a partir da matéria bruta (folhas e brotos) diluído no veículo hidroetanólico (EtOH: H2O / 50:50) para posterior análise microbiológica. Foram testadas a Concentração Inibitória Mínima (CIM) e Concentração Microbicida Miníma (CMM) do extrato vegetal, de acordo com as normas da Clinical and laboratory standards institute (CLSI), sobre quatro cepas: E. faecalis (ATCC e clínica) e C. albicans (ATCC e clínica). O extrato de P. guajava não produziu ação antifúngica sobre C. albicans, contudo, apresentou potencial microbicida contra cepas de E. faecalis, exibindo CIM de 0,20%. Esta concentração foi menor que a CIM do NaOCl que foi de 0,31%, uma solução que é comumente utilizada nas clínicas odontológicas. Em conclusão, o extrato hidroetanólico de P. guajava apresenta ação bactericida contra E. faecalis, sendo um produto natural com potencial para futuros estudos quanto ao desenvolvimento de novos irrigantes endodônticos.

El tratamiento endotónico consiste en la descontaminación del canal raíz con el uso de soluciones de irrigación, como el Hipoclorito de Sodio (NaOCl). Sin embargo, algunos microorganismos se resisten a las terapias convencionales, causando recaídas e infecciones secundarias. Es necesario estudiar las sustancias con poder de microbicida y el potencial para convertirse en nuevas soluciones de riego. Por lo tanto, el objetivo de este estudio fue evaluar el potencial antimicrobiano del extracto de Psidium guajava L. (guava) frente a cepas clínicas y estándar (ATCC) de Enterococcus faecalis y Candida albicans. Con este fin, el extracto se produjo a partir de la materia prima (hojas y brotes) diluida en el vehículo hidroetanólico (EtOH: H2O / 50:50) para análisis microbiológicos posteriores. La Concentración Mínima Inhibidora (CMI) y la Concentración de Microbicidas Minima (CCM) del extracto vegetal se probaron de acuerdo con el Clinical and laboratory standards institute (CLSI) en cuatro cepas: E. faecalis (ATCC y clínico) y C. albicans (ATCC y clínico). El extracto de P. guajava no produjo acción antifúngica sobre C. albicans, sin embargo mostró potencial microbicida frente a cepas de E. faecalis, presentando CIM 0,20%. Esta concentración fue menor que la CMI de NaOCl, que fue del 0,31%, una solución comúnmente utilizada en clínicas dentales. En conclusión, el extracto hidroetanólico de P. guajava muestra una acción bactericida frente a E. faecalis, siendo un producto natural con potencial para futuros estudios sobre el desarrollo de nuevos irrigantes endotónicos.

Neodexiopsis Malloch (Diptera, Muscidae) from Ecuador: description of new species and a key to the species

ABSTRACT Neodexiopsis Malloch (Diptera, Muscidae) is a member of the subfamily Coenosiinae, with more than a hundred species recorded in the Neotropical region. In Ecuador, it is known by four species, which may be an underestimated number. We describe five new species based on recently collected material: Neodexiopsis bicoloratta new species, N. longialata new species, N. nigrocalyptrata new species, N. plana new species, and N. secunda new species. A key to segregating all the recorded species from Ecuador is presented. The male and female terminalia of the new species were dissected and illustrated. Images of the habitus of the adult from the new species are presented.

Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production

Abstract The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single-rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.

Resumo O objetivo deste estudo foi avaliar in vitro o efeito do hidróxido de cálcio [Ca(OH)2], clorexidina gel a 2% (CHX) em macrófagos (RAW 264.7) na produção de citocinas pró-inflamatórias e óxido nítrico após pré-tratamento com ácido lipoteicóico (LTA) de Enterococcus faecalis. Quarenta e oito dentes humanos uniradiculares foram instrumentados com R25.08 (RECIPROC) e esterilizados por irradiação gama. LTA foi inoculado no canal radicular de cada espécime por 96 horas. Os espécimes foram instrumentados com 40.06 e 50.05 (RECIPROC) e medicados com: I) solução salina apirogênica (SS); II) CHX 2%; III) Ca(OH)2 + SS; ou IV) Ca(OH)2 + CHX durante 14 dias. Três amostras (S) foram realizadas do canal radicular de cada espécime em: S1) imediatamente após a instrumentação; S2) após ácido etilenodiaminotetracético (EDTA); S3) após a retirada da medicação intracanal. Foi realizada a quantificação subsequente de citocinas (IL-1β, TNF-α, MIP-1α, IP-10, G-CSF e IL-6) por imunoensaio (ELISA) e de óxido nítrico pelo método de Griess. Os dados foram submetidos a um teste de normalidade e então analisados com ANOVA one-way e teste de Tukey com nível de significância de 5% usando GraphPad Prism 6. Ca(OH)2 + SS e Ca(OH)2 + CHX apresentaram níveis mais baixos de TNF -α, TNF-α, IL-6, G-CSF e óxido nítrico. Ca(OH)2 + SS foi o mais eficaz na redução de MIP-1α. CHX foi eficaz na redução de IL-6 e G-CSF. Sendo assim a associação de hidróxido de cálcio e clorexidina é eficaz na redução das citocinas TNF-α, IL-1β, IL-6, G-CSF e óxido nítrico.

Taxonomy of the Neotropical species of Calythea (Anthomyiidae: Diptera), with description of two new species from South America

ABSTRACT Calythea Schnabl & Dziedzicki has 15 known species distributed worldwide, including three species in the neotropics. The species of Calythea can be easily identified by having bright silvery grey-dusting on the thorax and abdomen, forming a contrasting pattern with the dark body. Herein, we describe two new species from the Neotropical region and present new records of the genus for Argentina, Brazil, Chile, Colombia, Ecuador and Peru. We also redescribe Calythea comis (Stein). In addition, we present an identification key for the Neotropical species and drawings of the terminalia and habitus images of the new species, C. comis, C. crenata (Bigot) and C. micropteryx (Thomson).

Insect (Hexapoda) diversity in the oceanic archipelago of Fernando de Noronha, Brazil: updated taxonomic checklist and new records

ABSTRACT Hexapods, commonly known as insects, are a neglected taxonomic group in the Fernando de Noronha archipelago, with unanswered questions about their species richness and the ecological processes in which they are involved (e.g., colonization, introduction, establishment, and extinction). Herein, we provide an updated Hexapod checklist with current nomenclatural combinations. The entomofauna of the Fernando de Noronha archipelago is currently composed of 453 species in 21 orders. The orders, and their respective number of species, are: Blattaria (9), Coleoptera (118), Collembola (29), Dermaptera (3), Diplura (1), Diptera (134), Embioptera (1), Hemiptera (29), Hymenoptera (59), Isoptera (2), Lepidoptera (25), Mantodea (1), Neuroptera (3), Odonata (5), Orthoptera (11), Phasmatodea (1), Phthiraptera (6), Psocoptera (3), Siphonaptera (1), Thysanoptera (10), and Zygentoma (2). The archipelago has 263 new taxon records (family + genera + species). Thirty-eight species (3.39%) were described from local specimens and most of them are likely endemic species. This study more than doubles our knowledge (from the previous 190 records) of the entomofauna in this large Brazilian archipelago. This study also provides a baseline for studies on its conservation status and for implementing future environmental management programs.

Cordiluroides Albuquerque (Diptera: Muscidae): new species and key to the Neotropical genus

Abstract Here we describe three new species of Cordiluroides Albuquerque, one striking new species from Brazil, Minas Gerais, C. albitarsata sp. nov., and two from Peru, Cuzco, C. inca sp. nov. and C. wayqechensis sp. nov. Additionally, photographs from the types of C. listrata Albuquerque, 1954, C. megalopyga Albquerque, 1954 and C. insularis (Williston, 1896) are included. With the new species, Cordiluroides is now known from 11 species exclusively distributed in the Neotropical region with records from Bolivia, Brazil, Colombia, Costa Rica, Mexico, Saint Vincent, and Peru. We give an updated identification key to species from the Neotropical region. Finally, we describe for the first time the external morphology and genitalia of the Cordiluroides species using Scanning Electron Microscope (SEM) images.

Pictograph system for diagnosis making and data management in endodontics / Sistema de pictograma para elaboração de diagnóstico e gerenciamento de dados em endodontia

Objective: this questionnaire aimed to evaluate the approval of a new pictograph system for endodontic diagnosis by undergraduate and graduate students/endodontist instead of a conventional method. Methods: after a presentation to a total of 224 participants explaining the pictograph system, the participants (166 undergraduate students and 58 graduate students/endodontist) received a standardized questionnaire with three questions. The data were analyzed using the mult prop macro basing on Tukey's test for multiple comparison with (P ≤ 0.05). The first question was to define the education level of the participants; the second question about which diagnosis method is preferred; and the third question about the intention of using one of the methods in the future. The questionnaire was realized in May 2019. The response rate was 97.7%. Results: the first question showed that 74.10% and 25.90% were under graduate and graduate students/endodontist respectively. After statistical analysis there was no significant difference between the groups in the second and the third questions. In the second question (71.08% and 62.06%) of undergraduate and graduate student/endodontists respectively preferred the pictograph method over the conventional method. In the third question (60.24% and 51.72%) of undergraduate and graduate student/endodontists respectively showed interest in using the pictograph method in their professional career. Conclusion: the pictograph method in endodontic diagnosis is accepted by the majority of undergraduate and graduate students/endodontists. Training and experience affect the diagnosis making. (AU)

Objetivo: este questionário teve como objetivo avaliara aprovação de um novo sistema de imagem para diagnóstico endodôntico por estudantes de graduação e pós-graduação/endodontista, em vez de um método convencional. Métodos: após uma apresentação a um total de 224 participantes explicando o sistema de pictograma, os participantes (166 estudantes de graduação e 58 de pós graduação/endodontista) receberam um questionário padronizado com três perguntas. Os dados foram analisados usando a macro multi propósito baseada no teste de Tukey para comparação múltipla com (P ≤ 0,05). A primeira pergunta foi definir o nível de escolaridade dos participantes; a segunda pergunta sobre qual método de diagnóstico é preferido; e a terceira pergunta sobre a intenção de usar um dos métodos no futuro. O questionário foi realizado em maio de 2019. A taxa de resposta foi de 97,7%. Resultados: a primeira pergunta mostrou que 74,10% e 25,90% eram estudantes de graduação e pós-graduação / endodontista, respectivamente. Após análise estatística, não houve diferença significativa entre os grupos na segunda e na terceira questões. Na segunda questão (71,08% e 62,06%) dos estudantes de graduação e pós-graduação / endodontistas, respectivamente, preferiram o método de pictograma ao método convencional. Na terceira questão (60,24% e 51,72%) dos estudantes de graduação e pós-graduação /endodontistas, respectivamente, demonstraram interesse em utilizar o método de pictograma em sua carreira profissional. Conclusão: o método do pictograma no diagnóstico endodôntico é aceito pela maioria dos estudantes de graduação e pós-graduação / endodontistas. Treinamento e experiência afetam a elaboração do diagnóstico (AU)

Microbial profile and endotoxin levels in primary periodontal lesions with secondary endodontic involvement

Abstract This study was carried out to investigate the microbial profile and endotoxin levels of endodontic-periodontal lesions of periodontal origin. Periodontal and endodontic samples were taken from periodontal pockets and necrotic root canals of 10 teeth with endodontic-periodontal lesions. Evidencing of 40 different bacterial species were determined in each endodontic and periodontal sample using the checkerboard DNA-DNA hybridization method and Kinetic chromogenic LAL assay was used for quantification of endotoxins. Fisher's exact test correlated the bacterial species with the endodontic or periodontal microbiota. The endotoxin levels (EU/mL) found in samples of the root canal and periodontal pocket were compared by the Wilcoxon test (p<0.05). Bacteria and LPS units were found in 100% of the endodontic and periodontal samples. The species E. faecium, P. acnes, G. morbillorum, C. sputigena and L. buccalis were strongly correlated with the endodontic microbiota and P. nigrescens with the periodontal microbiota. P. intermedia, P. endodontalis and V. parvula were more prevalent in both endodontic and periodontal microbiots. The endotoxin levels in the periodontal pocket (89600 EU/mL) were significantly higher than in the root canal (2310 EU/mL). It was concluded that the microbiota present in the periodontal and endodontic tissues is similar, with a higher prevalence of species of the orange complex and a higher level of endotoxin in the periodontal pockets.

Resumo Este estudo foi realizado para investigar o perfil microbiano e os níveis de endotoxina de lesões endoperiodontais de origem periodontal. Amostras periodontais e endodônticas foram obtidas de bolsas periodontais e canais radiculares necróticos de 10 dentes com lesões endoperiodontais. A investigação de 40 espécies bacterianas diferentes foram determinadas em cada amostra endodôntica e periodontal usando o método de hibridização de DNA-DNA (checkerboard) e o ensaio cinético cromogênico LAL foi usado para quantificação de endotoxinas. O teste exato de Fisher correlacionou as espécies bacterianas com a microbiota endodôntica ou periodontal. Os níveis de endotoxina (EU/mL) encontrados nas amostras do canal radicular e na bolsa periodontal foram comparados pelo teste de Wilcoxon (p<0,05). Bactérias e unidades de LPS foram encontradas em 100% das amostras endodônticas e periodontais. As espécies E. faecium, P. acnes, G. morbillorum, C. sputigena e L. buccalis foram fortemente correlacionadas com a microbiota endodôntica e P. nigrescens com a microbiota periodontal. P. intermedia, P. endodontalis e V. parvula foram mais prevalentes em ambas microbiotas endodôntica e periodontal. Os níveis de endotoxina na bolsa periodontal (89600 EU/mL) foram significativamente maiores do que no canal radicular (2310 EU/mL). Concluiu-se que a microbiota presente nos tecidos periodontal e endodôntico é semelhante, com maior prevalência de espécies do complexo laranja e maior nível de endotoxina nas bolsas periodontais.

Inter-observer reproducibility of the "lumbar facet hypersignal classification" and the classification of pfirrmann / Reprodutibilidade inter observadores da "classificação de hipersinal facetário lombar" e da classificação de pfirrmann / Reproductividad inter-observadores de la "clasificación de hiperseñal facetaria lumbar" y de la clasificación de pfirrmann

ABSTRACT Objective: To evaluate the inter-observer relationship of the "lumbar facet hypersignal classification" and the correlation with disc degeneration. Methods: Retrospective study of magnetic resonance images obtained from 24 (N=24) patients (9 males and 15 females), aged 35 to 79 years, mean age 48 years and 1 month. The images were reviewed by two spine surgeons, by five orthopedists in training in the specialty of spine surgery and one radiologist to evaluate and quantify the presence of hypersignal lumbar facet and the Pfirrmann Classification for disc degeneration. Results: One hundred and twenty lumbar discs and their joint facets were evaluated by the eight examiners, with data analyzed by the Cronbach test and the Spearman Correlation Analysis with statistically high results, confirming good inter-observer relation for the "Lumbar Facet Hypersignal Classification" and for the classification of Pfirrmann. There was no statistically significant relationship between facet arthrosis and disc degeneration. Conclusions: The results confirm that there is good inter-observer relationship for the classification of Facet Hypersignal and for the Classification of Pfirrmann. However, in spite of a positive relationship, a correlation between facet arthrosis and disc degeneration was not statistically significant. Level of Evidence III; Retrospective Comparative Study.

RESUMO Objetivo: Avaliar a relação inter observadores da "classificação de hipersinal facetária lombar" e a correlação com a degeneração discal. Métodos: Estudo retrospectivo de imagens de ressonância magnética obtidas de 24 (N= 24) pacientes (nove homens e 15 mulheres) com idade variando de 35 a 79 anos, com média de de 48 anos e um mês. As imagens foram revisadas por dois ortopedistas especialistas em cirurgia de coluna, por cinco ortopedistas em treinamento na especialidade de cirurgia de coluna e um radiologista, para avaliar e quantificar a presença de hipersinal facetário lombar e da Classificação de Pfirrmann para degeneração discal. Resultados: Foram avaliados e classificados 120 discos lombares e suas facetas articulares pelos oito examinadores, com dados analisados pelo Teste de Conbach e da Análise de Correlação de Spearman com resultados estatisticamente elevados, confirmando boa relação inter observadores para a "Classificação de Hipersinal Facetario Lombar" e para a classificação de Pfirrmann. Não foi observada relação estatisticamente significante entre artrose facetária e degeneração fiscal. Conclusão: Os resultados confirmam que existe boa relação inter observadores para a classificação de Hipersinal Facetário e para a Classificação de Pfirrmann. Porém, apesar de relação positiva, não se atingiu de maneira estatisticamente significante, correlação entre artrose facetaria e degeneração discal. Nível de Evidência III; Estudo Restrospectivo Comparativo.

RESUMEN Objetivo: Evaluar la relación inter-observadores de la "clasificación de hiperseñal facetaria lumbar" y la correlación con la degeneración de disco. Métodos: Estudio retrospectivo de imágenes de resonancia magnética obtenidas de 24 (N = 24) pacientes (9 hombres y 15 mujeres), con edad variando de 35 a 79 años, con promedio de 48 años y 1 mes. Las imágenes fueron revisadas por dos ortopedistas especialistas en cirugía de columna, por cinco ortopedistas en entrenamiento en la especialidad de cirugía de columna y un radiólogo para evaluar y cuantificar la presencia de hiperseñal facetaria lumbar y la Clasificación de Pfirrmann para degeneración de disco. Resultados: Fueron evaluados y clasificados 120 discos lumbares y sus facetas articulares por los ocho examinadores, con datos analizados por el Test de Cronbach y del Análisis de Correlación de Spearman con resultados estadísticamente elevados, confirmando buena relación inter-observadores para la "Clasificación de Hiperseñal Facetaria Lumbar" y para la clasificación de Pfirrmann. No se observó relación estadísticamente significativa entre artrosis facetaria y degeneración de disco. Conclusiones: Los resultados confirman que existe una buena relación inter-observadores para la clasificación de Hiperseñal Facetaria y para la Clasificación de Pfirrmann. Sin embargo, a pesar de una relación positiva, la correlación entre la artrosis facetaria y la degeneración de disco no fue estadísticamente significativa. Nivel de Evidencia III; Estudio Retrospectivo Comparativo.

New systematic position of Itatingamyia Albuquerque (Diptera, Muscidae) based on molecular evidence, and description of the female of I. couriae

ABSTRACT ItatingamyiaAlbuquerque, 1979 is rare in collections, with only nine specimens preserved in museums. Two species are known, and their placement within Muscidae was never tested using molecular data. Here, we estimate the position of Itatingamyia within Muscidae with mitochondrial (COI) and nuclear (AATS, CAD, and EF1-α) markers using Maximum likelihood and Bayesian posterior probabilities as optimality criteria. According to our results, we propose to classify Itatingamyia as a Cyrtoneurininae. We also describe the previously unknown female and egg of Itatingamyia couriaeHaseyama and de Carvalho, 2011 and expand the known distribution of this species to the state of Minas Gerais, Brazil, through the discovery of 13 new specimens there. We also argue that Itatingamyia distribution is restricted to Atlantic Forest areas.

Taxonomic revision of the Neotropical stalk-eyed fly Plagiocephalus Wiedemann (Diptera, Ulidiidae, Ulidiinae)

ABSTRACT Plagiocephalus is a genus composed of three species mainly recognized by the males with stalked-eyes. Plagiocephalus lobularis is distributed from Northeastern Brazil to North of Argentina, P. latifrons is distributed from Mexico to Bolivia, and P. intermedius occurs in Costa Rica. We review the species of the genus, providing new diagnostic characters, an identification key, and new information on the terminalia of Plagiocephalus. Also, we update the geographic distribution of the species of the genus.

Endodontic treatment of a large periapical cyst with the aid of antimicrobial photodynamic therapy: case report / Tratamento endodôntico de um grande cisto periapical com o auxílio de terapia fotodinâmica antimicrobiana: relato de caso

Introduction: Periapical cysts, especially those of great extension, represent a challenge for endodontics, due to the ineffectiveness of traditional root canal treatment (RCT) in such cases. The aim of this case report was to evaluate the effectivity of antimicrobial photodynamic therapy "aPDT" when associated with calcium hydroxide Ca(OH)2 in treating such cysts. Case report: A 40-years-old female patient complained of bulging in the upper anterior region of the mouth. The clinical examination showed an extensive swollen periodontal area, painful with digital palpation, with a negative response to thermal tests on teeth 11 and 21. After traditional RCT, the canals of teeth 11 and 21 were filled with photosensitizer methylene blue 0.005% for 5 min pre irradiation time (PIT) and irradiated by low power diode laser with visible red wavelength (660 nm) during 2 minutes without intervals, using an optical fiber delivering system with a helical movement from apical to cervical. Then the canals were filled with Ca(OH)2 paste for 45 days and had been changed each 15 days, and repeating the radiation. Results: After 45 days, the patient already had a bulging remission, with painless resistance to the apical pressure over the region and signs of bone repair confirmed with the radiographic examination, and total absence of signs or symptoms. Conclusion: the association of aPDT with Ca(OH)2 paste is effective in treating of periapical lesions of great extension and avoids surgical treatment. (AU)

Introdução: os cistos periapicais, especialmente os de grande extensão, representam um desafio para a endodontista, uma vez que não são reparados com o tratamento endodôntico tradicional. O objetivo deste relato de caso foi avaliar a efetividade da terapia fotodinâmica antimicrobiana "aPDT" quando associada ao hidróxido de cálcio Ca(OH)2 no tratamento desses cistos. Relato de caso: paciente do sexo feminino, 40 anos, com queixa de abaulamento na região ântero-superior da boca. O exame clínico evidenciou extensa área periodontal inchada, dolorosa com palpação digital, com resposta negativa aos testes térmicos dos dentes 11 e 21. Após o tratamento endodôntico tradicional, os canais dos dentes 11 e 21 foram preenchidos com fotossensibilizador de azul de metileno a 0,005% de tempo de pré-radiação de 5 min e irradiados por laser de diodo de baixa potência com comprimento de onda vermelho visível (660 nm) por 2 min sem intervalos, usando um sistema de fornecimento de fibra ótica com um movimento helicoidal do apical ao cervical. Em seguida, o canal foi preenchido com pasta de Ca(OH)2 por 45 dias sendo trocados a cada 15 dias e repetida a radiação. Resultados: Após 45 dias, o paciente já apresentava uma remissão volumosa, com resistência indolor à pressão apical na região e sinais de reparo ósseo ao exame radiográfico e ausência total de sinais ou sintomas. Conclusão: a associação de aPDT com Ca(OH)2 é eficaz no tratamento de lesões periapicais de grande extensão e evitar tratamento cirúrgico (AU)

Acting and knowledge of emergency rescue teams in dental trauma / Atuação e conhecimento de equipes de resgate de emergência em casos de trauma dental

Objetivo: O traumatismo dentário é regularmente freqüente em pacientes atendidos por equipes de resgate em locais de acidentes e por equipes de emergência em hospitais. No entanto, esses profissionais recebem pouca ou nenhuma informação sobre como cuidar e atuar em casos de lesões traumáticas em tecidos duros e moles. O objetivo deste estudo foi avaliar o nível de conhecimento dos profissionais das equipes de resgate e emergência (médicos, enfermeiros e paramédicos) sobre o atendimento do traumatismo dentário. Material e métodos: 196 profissionais das unidades de resgate e emergência receberam um questionário padronizado de 5 questões referentes ao atendimento do traumatismo dentário e preservação de tecido dentário avulsionado (um dente ou seu fragmento). Resultados: os resultados mostraram um alto nível de atendimento de pacientes de traumatismo dentário por paramédicos, enfermeiros e médicos, com conhecimento muito baixo sobre como lidar com esses casos, e com resultados moderados sobre o melhor meio para preservar o dente avulsionado, e as ações tomadas durante o processo de resgate. Conclusão: há uma alta ocorrência de casos de traumatismos dentários atendidos por equipes de resgate e emergência, com falta de conhecimento, o que sugere a necessidade de mais campanhas educativas para esses profissionais com acompanhamento a longo prazo. (AU)

Male and female association in Trichomyia Haliday in Curtis, 1839 using a molecular approach (Diptera, Psychodidae, Trichomyiinae), and description of new species from Brazil

ABSTRACT A new species of Trichomyia from the state of Bahia, Brazil, is described and illustrated, and male and female are associated using DNA barcoding. Additionally, fragments of the COI of two other species, Trichomyia cerdosa Araújo & Bravo, 2016 and Trichomyia ituberensis Araújo & Bravo, 2016, and the females of two unidentified species, are sequenced.

A new species of Drepanocnemis (Diptera, Muscidae) from Andes in Peru, with an updated phylogenetic analysis of species

ABSTRACT Drepanocnemis Stein (Diptera, Muscidae) is a small genus of flies that occur in high altitudes in the Colombian Andes, Ecuador, Peru and Bolivia. Herein we describe Drepanocnemis aurifrons sp. nov. from Cuzco, Peru, which is found from high (2904 m) to lower altitudes (707 m). An updated phylogeny, key to species and map of species' distributions are provided, together with images and illustrations of the male and the female terminalia.

Two new species of Trichomyia Haliday 1839 (Diptera, Psychodidae, Trichomyiinae) from the Pantanal of Mato Grosso, Brazil

Abstract Two new species in the Trichomyiinae (Psychodidae), Trichomyia pantanensis sp. nov. and Trichomyia lamasi sp. nov., are described and illustrated. New records are given for the two additional species Trichomyia spinicauda Araújo & Bravo, 2016 and Trichomyia hispida Araújo & Bravo, 2016. These four species comprise the first records of the genus in the Pantanal region.

Performance of sunflower cultivars at different seeding periods in central Brazil / Desempenho de cultivares de girassol em diferentes épocas de semeadura no Brasil central

ABSTRACT Sunflower (Helianthus annuus L.) is a very important crop for Brazil, and especially for Chapada do Parecis which is the largest domestic producer, because of its climate and soil conditions are adequate to the development of the specie. The performance of cultivars and sunflower seed sowing periods were evaluated in Central Brazil. Experimental design comprised an 8 x 5 factorial randomized complete block, with eight sunflower cultivars (Aguará 06, Aguará 04, MG 305, CF 101, SYN 3950HO, SYN 045, GNZ NEON and HELIO 251) and five decennial seeding periods, with three replicates. Analysis of variance and Scott-Knott´s test (p<0.05) were applied. Cultivars GNZ NEON and SYN 045 provide the highest production rates in achenes, with higher value in favor of GNZ NEON. SYN 3950HO (this with higher value), CF101, MG 305, SYN 045 and Aguará 04 gets the highest oil rates in the achenes, with SYN 045, GNZ NEON, Aguará 04, CF101 and SYN 3950HO with the highest oil production. The first seed sowing period is the most productive and the first and fifth periods have the best results for oil productivity. Cultivar GNZ NEON and the first period exceede the others with regard to vegetative and reproductive variables.

RESUMO O girassol (Helianthus annuus L.) é uma cultura de grande importância para o Brasil, e especialmente para Chapada do Parecis, que é o maior produtor nacional, em virtude das suas condições edafoclimáticas serem propicias ao desenvolvimento da espécie. Objetivou-se avaliar o desempenho de cultivares e épocas de semeadura do girassol no Brasil Central. O delineamento experimental utilizado foi o de blocos casualizados, em esquema fatorial 8 x 5, sendo oito cultivares de girassol (Aguará 06, Aguará 04, MG 305, CF 101, SYN 3950HO, SYN 045, NEON e HELIO 251) e cinco épocas decendiais de semeadura, com três repetições. Aplicou-se análise de variância e teste Scott-Knott (p<0,05). As cultivares GNZ NEON e SYN 045 proporcionaram maiores produtividades de aquênios, com valor superior em favor da GNZ NEON. A SYN 3950HO (esta com valor superior), CF101, MG 305, SYN 045 e Aguará 04, obtiveram os maiores teores de óleo nos aquênios. A 1ª época de semeadura foi a mais produtiva, assim como a 1ª e a 5ª épocas apresentaram os melhores resultados para produtividade de óleo. A cultivar GNZ NEON e a 1ª época foram as que se destacaram das demais, tanto para as variáveis vegetativas como para as reprodutivas.

Copper II - polar amino acid complexes: toxicity to bacteria and larvae of Aedes aegypti

ABSTRACT Control strategies using insecticides are sometimes ineffective due to the resistance of the insect vectors.In this scenario new products must be proposed for the control of insect vectors.The complexes L-aspartate Cu (II) and L-glutamate-Cu (II) complexes were synthesized and characterized by elemental analysis, visible ultraviolet, infrared spectroscopy and potentiometric titration. The toxicity of these complexes was analyzed in Aedes aegypti (Diptera: Culicidae) larvae and Gram-negative and Gram-positive bacteria. The interaction between the ligands and the amino acid balance and the distribution of the species as a function of pH were discussed. The lethal concentration median (LC50) for Ae. aegypti larvae were: L-glutamic acid-Cu (II) - 53.401 mg L-1 and L-aspartate-Cu (II) - 108.647 mg L-1. The minimum inhibitory concentration (MIC) required for Staphylococcus aureus and Escherichia coli was: L-glutamate-Cu (II) 500-2000 mg L-1 and L-aspartate-Cu (II) 1000-2000 mg L-1. The concentrations demonstrated toxicity that evidence the potential of the complexes as bactericide and insecticide. Metal complexes formed by amino acids and transition metals are advantageous because of low environmental toxicity, biodegradability and low production cost.