Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Añadir filtros








Intervalo de año
1.
Mem. Inst. Oswaldo Cruz ; 113(8): e170452, 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-955116

RESUMEN

BACKGROUND Malaria is responsible for 429,000 deaths per year worldwide, and more than 200 million cases were reported in 2015. Increasing parasite resistance has imposed restrictions to the currently available antimalarial drugs. Thus, the search for new, effective and safe antimalarial drugs is crucial. Heterocyclic compounds, such as dihydropyrimidinones (DHPM), synthesised via the Biginelli multicomponent reaction, as well as bicyclic compounds synthesised from DHPMs, have emerged as potential antimalarial candidates in the last few years. METHODS Thirty compounds were synthesised employing the Biginelli multicomponent reaction and subsequent one-pot substitution/cyclisation protocol; the compounds were then evaluated in vitro against chloroquine-resistant Plasmodium falciparum parasites (W2 strain). Drug cytotoxicity in baseline kidney African Green Monkey cells (BGM) was also evaluated. The most active in vitro compounds were evaluated against P. berghei parasites in mice. Additionally, we performed an in silico target fishing approach with the most active compounds, aiming to shed some light into the mechanism at a molecular level. RESULTS The synthetic route chosen was effective, leading to products with high purity and yields ranging from 10-84%. Three out of the 30 compounds tested were identified as active against the parasite and presented low toxicity. The in silico study suggested that among all the molecular targets identified by our target fishing approach, Protein Kinase 3 (PK5) and Glycogen Synthase Kinase 3β (GSK-3β) are the most likely molecular targets for the synthesised compounds. CONCLUSIONS We were able to easily obtain a collection of heterocyclic compounds with in vitro anti-P. falciparum activity that can be used as scaffolds for the design and development of new antiplasmodial drugs.


Asunto(s)
Diseño de Fármacos , Pruebas de Sensibilidad Parasitaria , Antimaláricos/síntesis química , Antimaláricos/farmacología , Pirimidinonas , Pirroles
2.
Mem. Inst. Oswaldo Cruz ; 101(5): 523-528, Aug. 2006. ilus, graf, tab
Artículo en Inglés | LILACS | ID: lil-437036

RESUMEN

Immune responses to malaria infections are characterized by strong T and B cell activation, which, in addition of potentially causing immunopathology, are of poor efficacy against the infection. It is possible that the thymus is involved in the origin of immunopathological reactions and a target during malaria infections. This work was developed in an attempt to further clarify these points. We studied the sequential changes in the thymus of CBA mice infected with Plasmodium berghei ANKA, a model in which 60-90 percent of the infected animals develop cerebral malaria. During the acute phase of infection, different degrees of thymocyte apoptosis were recorded: (1) starry-sky pattern of diffuse apoptosis with maintenance of cortical-medullary structure; (2) intense apoptosis with cortical atrophy, with absence of large cells; (3) severe cortical thymocyte depletion, resulting in cortical-medullary inversion. In the latter, only residual clusters of small thymocytes were observed within the framework of epithelial cells. The intensity of thymus alterations could not be associated with the degree of parasitemia, the expression of clinical signs of cerebral malaria or intensity of brain lesions. The implications of these events for malaria immunity and pathology are discussed.


Asunto(s)
Animales , Femenino , Ratones , Apoptosis/inmunología , Malaria Cerebral/inmunología , Malaria Cerebral/parasitología , Plasmodium berghei/fisiología , Timo/inmunología , Modelos Animales de Enfermedad , Depleción Linfocítica , Ratones Endogámicos CBA , Malaria Cerebral/patología , Parasitemia , Índice de Severidad de la Enfermedad , Factores de Tiempo , Timo/patología
3.
Mem. Inst. Oswaldo Cruz ; 95(3): 363-5, May-Jun. 2000. graf
Artículo en Inglés | LILACS | ID: lil-258190

RESUMEN

Aotus is one of the WHO-recommended primate models for studies in malaria, and several species can be infected with Plasmodium falciparum or P. vivax. Here we describe the successful infection of the species A. infulatus from eastern Amazon with blood stages of P. falciparum. Both intact and splenectomized animals were susceptible to infection; the intact ones were able to keep parasitemias at lower levels for several days, but developed complications such as severe anemia; splenectomized monkeys developed higher parasitemias but no major complications. We conclude that A. infulatus is susceptible to P. falciparum infection and may represent an alternative model for studies in malaria.


Asunto(s)
Animales , Masculino , Femenino , Modelos Animales de Enfermedad , Haplorrinos/parasitología , Malaria Falciparum/parasitología , Enfermedades de los Monos/parasitología , Plasmodium falciparum/inmunología , Temperatura Corporal , Susceptibilidad a Enfermedades , Haplorrinos/inmunología , Enfermedades de los Monos/inmunología , Parasitemia/parasitología , Esplenectomía
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA