Effects of colchicine on oxytocin-and vasopressin-containing neurons in the supraoptic nucleus of rats of different ages

The mitotic inhibitor colchicine inhibits the axonal flow of proteins in various types of neurons. A differential effect of colchicin e on vasopressin-containing neurons compared to oxytocin-containing ones had been suggested, and a decline

Experimental microneurosurgery of the central and peripheral nervous system in the study of the neuronal and glial trophism and plasticity

Trauma and neurodegenerative diseases commit the nervous system. After an axotomy or nerve injury in the peripheral nervous system the regeneration of the nerve fibers and reinervation of the target are seen. In central nervous system these events are restrictive, however their occurrence are related to the state of glial reaction and the synthesis of neurotrophic factors. Basic fibroblast growth factor (bFGF) has been considered an important trophic factor for neurons and astrocytes of many central nervous system regions. In this study rats were submitted to one of following neurosurgery procedures: callosotomy, pyramidectomy or complete transection of hypoglossal nerve (XII). Sham operations were made in control animals. Seven days later animals were sacrificed and their braims processed for immunohistochemistry. Coronal sections were taken from the central nervous system and incubated with antisera against the glial fibrillary acidic protein (GFAP) or neurofilament (NF), markers for astrocyte and neuronal cell body and fibers, respectively, as well as with the antiserum against the bFGF. The degree of the labelling was quatified with computer assisted stereological methods. The analysis of the NF immunoreactivity revealed a disappearance of fibers in the white matter distal to the pyramidectomy and callosotomy, however no disapperance of NF immunoreactive neurons was found in the XII nucleus following axotomy. These changes was accompanied by a massive astrocytic reaction. The reactive astrocytes synthesized increased amounts of bFGF. These findings suggest that glial reaction synthesizing neurotrophic factors may influence the wound and repair after mechanical lesions of central nervous and subsequent neuronal trophism and plasticity which may be relevant to the regenerative process of the nervous tissue.

Incidencia de amputacao de membro inferior, unilateral: analise de prontuarios / Incidence of lower limb amputation, one side: medical history analisis

Amputacao e a perda ou retirada de um membro pela cirurgia, traumatismos e doencas. Diversos fatores influem tanto na opcao por este procedimento, quanto na recuperacao do paciente apos a cirurgia. Acredita-se que estes fatores estejam relacionados ao nivel da amputacao, a idade, o sexo, e biotipo do paciente, bem como da condicao socio-economica, cultural e do sistema de saude local. O objetivo deste trabalho foi identificar e correlacionar os fatores que influem na reabilitacao de pacientes com amputacao de membro inferior, unilateral, abaixo ou acima do joelho atraves da analise de prontuarios...