1.
Indian J Exp Biol
;
1997 Jan; 35(1): 86-8
Artículo
en Inglés
| IMSEAR
| ID: sea-61869
RESUMEN
In the present study, a rapid and simple colorimetric technique has been described to determine the presence of bacteria in tissue culture medium used in animal cell culture. The microplate assay is based on utilization of MTT [3(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] by bacteria resulting in formation of formazan crystals which can be measured colorimetrically. Contaminated medium, a standard gram-negative and gram-positive bacteria produce formazan from MTT which is related to the bacterial load. The assay has utility in screening tissue culture reagents to detect the presence of bacteria.
Asunto(s)
Bacterias/aislamiento & purificación , Técnicas Bacteriológicas/métodos , Cristalización , Medios de Cultivo/análisis , Contaminación de Medicamentos , Formazáns/metabolismo , Sales de Tetrazolio/metabolismo , Tiazoles/metabolismo
2.
J Indian Med Assoc
;
1957 Oct; 29(7): 287-8
Artículo
en Inglés
| IMSEAR
| ID: sea-103378