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Indian J Biochem Biophys ; 2006 Aug; 43(4): 244-6
Artículo en Inglés | IMSEAR | ID: sea-26910

RESUMEN

Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for Dl protein.


Asunto(s)
Secuencia de Bases , Bioquímica/métodos , Northern Blotting , Cloroplastos/metabolismo , ADN Complementario/metabolismo , ADN de Plantas , Etiquetas de Secuencia Expresada , Perfilación de la Expresión Génica , Genes de Plantas , Datos de Secuencia Molecular , Oryza/enzimología , Complejo de Proteína del Fotosistema II/química , Polimorfismo Genético
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