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AIM: To contrast the effect of small incision phacoemulsification and non-phacoemulsification to treat cataract. METHODS: Totally 172 patients with cataract were divided into 2 groups ( n = 86 ) randomly. Patients in Group Phaco were treated with phacoemulsification and, while those in Group Siecs were treated with small incision extracapsular cataract surgery and intraocular lens implantation. They were all followed up for 1-3y. Functional examinations were made at 3d;1,6mo and last follow - up. The visual acuity, intraocular pressure, anterior chamber depth, average corneal power ( ACP ) , cylinder ( CYL ) , surface asymmetry index ( SAI ) and complications were contrasted between groups. RESULTS: At 3d after operation, the visual acuity of Group Phaco was better than that of Group Siecs ( P 0. 05 ). Furthermore, the APC and anterior chamber depth of each point- in - time had no significant difference between groups (P>0. 05). The incidences of complication were similar in two groups ( P> 0. 05 ); but with individual differences, patients of GradeII and Ⅲ in Group Phaco got lower complication rate (PCONCLUSION: Our research shows that phacoemulsification has high effect in the treatment of patients with GradeII and Ⅲ nuclear hardness, while small incision extracapsular cataract surgery has high effect in the treatment of patients with Grade Ⅳ nuclear hardness.
RESUMEN
OBJECTIVE: To establish an HPLC-MS/MS method (high performance liquid chromatography-tandem mass spectrometry) for the direct determination of amifostine in human saliva. METHODS: Saliva samples were collected from six adult healthy volunteers. After protein precipitation and addition of the internal standard (IS) huperzine-A (HupA), HPLC-MS/MS was used to analyze amifostine. The analysis was conducted using a ZIC-HILIC analytical column (2.1 mm × 100 mm, 3.5 μm). Electrospray ionization was used with multiple reaction monitoring (MRM) mode. RESULTS: The lower limit of quantification (LLOQ) of the method was 0.938 mg · L-1 (S/N<10). The standard curve was linear in the range of 0.938-30 mg · L (r=0.9991, n=6). The inter-day and intra-day RSDs were all less than 15% for the low, medium and high concentration quality control samples (1.0, 5.0 and 25 mg · L-1): The values of recovery were all more than 85%. CONCLUSION: The method is direct, rapid, simple and sensitive, and suitable for the determination of amifostine in saliva samples.