Effects of recombinant adenovirus-mediated hypoxia-inducible factor-1alpha gene on proliferation and differentiation of endogenous neural stem cells in rats following intracerebral hemorrhage

OBJECTIVE@#To investigate the effects of adenovirus (Ad)-mediated hypoxia-inducible factor-1alpha (HIF-1α) gene on proliferation and differentiation of endogenous neural stem cells (NSCs) in rats following intracerebral hemorrhage (ICH) and the underlying mechanisms.@*METHODS@#A total of 120 specific pathogen-free, adult, male Sprague-Dawley rats were included in this study. After establishment of ICH models in rats, PBS, Ad, or Ad-HIF-1α was administered via the ischemic ventricle. On the 1st, 7th, 14th, 21st and 28th d after ICH, rat neurological deficits were scored, doublecortin (DCX) expression in the subventricular zone cells was detected by immunohistochemical staining, and 5-bromo-2'-deoxyuridine (BrdU)-, BrdU/DCX-, and BrdU/glial fibrillary acidic protein-positive cells in the subventricular zone were counted using immumofluorescence method among PBS, Ad, and Ad-HIF-1α groups.@*RESULTS@#On the 7th, 14th, 21st and 28th d after ICH, neurological deficit scores in the Ad-HIF-1α group were significantly lower than in the PBS and Ad groups (P<0.05). In the Ad-HIF-1α group, DCX expression was significantly increased on the 7th d, peaked on the 14th d, and then gradually decreased. In the Ad-HIF-1α group, BrdU-positive cells were significantly increased over time course, and significant difference in BrdU-positive cell counts was observed when compared with the PBS and Ad groups at each time point (P<0.01 or 0.05). On the 7th, 14th, 21st and 28th d after ICH, the number of DCX-, BrdU-, BrdU/DCX-, and BrdU/DCX-positive cells in the Ad-HIF-1α group was significantly greater than in the PBS and Ad groups (P<0.05).@*CONCLUSIONS@#HIF-1α gene can promote the proliferation, migration and differentiation of endogenous neural stem cells after ICH, thereby contributing to neurofunctional recovery after ICH.

Prevalence and correlation factors of carotid atherosclerosis among the middle and old aged workers in an Iron and Steel corporation, Chongqing / 中华流行病学杂志

Objective To investigate the prevalence and correlation factors of carotid atherosclerosis (CAS) among workers of Chongqing Iron and Steel Corporation, and to offer information for the development of prevention program for cerebro-vascular disease. Methods 512 asymptomatic individuals (mean age 56.8 years, ranged from 45 to 80) from the subsidiary workshop of Chongqing Iron and Steel Corporation were recruited. Demographic data and serum were collected and the internal-media thickness (IMT) of the CAS and the extent of plaque formation were evaluated by B-mode ultrasound. Logistic regression analysis was used to estimate the correlation factors of CAS. Results (1) The overall prevalence of carotid atherosclerosis among the iron and steel workers was 37.6%. IMT was found as 10.2% , with the prevalence rates of nonstenotic plaque and stenotic plaque were 25.0% and 2.4% respectively. (2)It was shown by logistic regression analysis that age (0R=1.289, 95%CI: 1.014-1.568, P<0.001 ), smoking (0R=1.420, 95%C1: 0.802-3.872, P<0.001 ), hypertension (0R=4.530, 95%CI: 3.952-6.753, P<0.001), diabetes mellitus (0R=2.285, 95%C1: 1.008-5.057, P<0.001 ), HsCRP(0R= 1.273, 95%C1: 0.479-2.889, P=0.037), TC (0R= 1.032,95%C1: 0.320-1.882,P=0.047) and LDL-C (0R=2.313, 95% CI:1.237-4.331, P=0.008) were significantly associated with the severity of CAS. (3)Prevalence of the CAS increased with the increasing number of correlation factors. Conclusion The prevalence of CAS among the iron and steel workers was higher than those in ordinary people of the same age. Age, smoking, hypertension, diabetes mellitus, HsCRP, TC and LDL-C were independent risk factors related to CAS. The prevalence of CAS was increasing parallel to the number of correlation factors.

The Application of Transcranial Color-Coded Sonography in Cerebrovascular Diseases / 国际脑血管病杂志

Transcranial Doppler combined with flow imaging technique can conveniently,nonin- vasively and accurately detect the blood flow in large intracranial vessels.It has practical signifi- cance in the diagnosis and treatment of cerebrovascular diseases.This article reviews the appli- cation of transcranial color-coded sonography(TCCS)in cerebrovascular diseases.

Study in the mechanism of Shuxuetong injection on antithrombosis and thrombolysis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of Shuxuetong injection on cerebral tissue and brain microvascular endothelial cell(BMEC) in secreting tissue-type plasminogen activator (tPA).</p><p><b>METHOD</b>The experiment in vivo was designed for three groups: sham-operation group, focal cerebral ischemia model group, Shuxuetong injection-treated group. The gene expression and the activity of tPA on brains were detected by RT-PCR and chromogenic substrate analysis respectively after treatment for five days; The experiment in vitro: The cultured BMEC were divided into experimental group and control group. The Shuxuetong injection were added into experimental group and the BMECs in the experimatal groups were treated for 24 h. The gene expression and the activity of tPA on cultured BMEC were detected by RT-PCR and chromogenic substrate analysis respectively.</p><p><b>RESULT</b>The gene expression and the content of tPA on both cerebral tissue and BMEC in the experimatal groups were significantly higher than that in the control groups.</p><p><b>CONCLUSION</b>Shuxuetong injection could accelerate secretion of tPA, and therefore enhance fibrinolysis.</p>