Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
Chinese Journal of Virology ; (6): 424-428, 2007.
Artículo en Chino | WPRIM | ID: wpr-334871

RESUMEN

The G1 cytoplasmic tail of Hantaan virus (HTNV) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (ITAM) and is termed the ITAM-like sequence. To demonstrate the potential signal-transducing activity of G1 ITAM-like sequence resembling the canonical ITAM within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. The synthesized G1 ITAM-like peptide was shown to coprecipitate with cellular phosphoprotein complexes by an immune-complex kinase assay. Mutational analyses showed that this ITAM-like sequence was a substrate for the Src family kinase Fyn, and two conserved tyrosine residues were required for coprecipitating Lyn, Syk, and ZAP-70 kinases. These findings demonstrated that HTNV envelope glycoprotein G1 contains a functional ITAM-like sequence in its cytoplasmic tail, which can bind critical cellular kinases that regulate immune and endothelial cell functions.


Asunto(s)
Humanos , Secuencia de Aminoácidos , Células Cultivadas , Virus Hantaan , Química , Fisiología , Péptidos y Proteínas de Señalización Intracelular , Fisiología , Datos de Secuencia Molecular , Fosforilación , Proteínas Tirosina Quinasas , Fisiología , Proteínas Proto-Oncogénicas c-fyn , Fisiología , Transducción de Señal , Quinasa Syk , Proteínas del Envoltorio Viral , Química , Fisiología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA