Screening for UV-C irradiation-enhanced transcription factors that regulate the metabolism of phenolic compounds in tomato fruit / 生物工程学报

Solanum lycopersicum phenylalanine ammonia-lyase 5 (SlPAL5) gene regulates the metabolism of phenolic compounds. The study of transcription factors that regulate the expression of SlPAL5 gene is of great significance to elucidate the regulatory mechanism underlying the biosynthesis of phenolic compounds in tomato fruit induced by UV-C irradiation. Here, yeast one-hybrid library of tomato fruit was constructed, and the yeast one-hybrid technology was used to screen the transcription factors that regulate the expression of SlPAL5, the key gene related to the synthesis of phenolic compounds in tomato fruit. As a result, a transcription factor, SlERF7, was obtained and sequenced, followed by the blast homology analysis. Further experiments confirmed that SlERF7 interacted with the promoter of SlPAL5 gene. In addition, UV-C irradiation significantly increased the expression level of SlERF7. These results indicate that SlERF7, which is regulated by UV-C irradiation, might be involved in regulating the transcription of SlPAL5, which provided foundations for further studying the regulation mechanism of the biosynthesis of phenolic compounds in tomato fruit induced by UV-C irradiation.

Pathogen isolation and identification of an outbreak of infection in lung with unknown causes / 中国人兽共患病学报

We investigated the pathogen of an outbreak of lung infection with unknown causes.By epidemiological analysis,we used real-time PCR,ELISA,gold dipstick,VITEK2 and MALDI-TOF-MS to identify suspicious bacteria.We made use of serum plate agglutination test to confirm the suspicious bacteria and the patient serum.We isolated 2 strains of Cryptococcus albidus from environmental samples.There has been specific agglutination between suspicious bacteria and patient serum.This pneumonia may be related to the infection of Ccryptococcus albidus.

Aspirin effects on MG-63 cell proliferation on different modified titanium surfaces under fluid shear stress / 中国组织工程研究

BACKGROUND:Recent research has shown that the proper concentration of aspirin can increase the proliferation and osteogenic ability of MG-63 cells.OBJECTIVE:To explore the effects of different concentrations of aspirin on osteoblast proliferation on the implant-cell interface under fluid shear stress.METHODS:(1) MG-63 cells were cultured in low-glucose DMEM containing 10％ fetal bovine serum and different concentrations of aspirin (0,0.023,0.046,0.0625,0.125,0.25,0.5,1.0,2.0,4.0 mmol/L) for 1-7 days.Then cell proliferation was detected using MTS method.(2) MG-63 cells were cultured on three different surfaces:glass slide,PT titanium surface and SLA titanium surface.After 3 days of culture with aspirin at a concentration of 0 or 0.5 mmol/L,the cells were subjected to fluid shear stress.MTS test was applied to estimate the proliferation of MG-63 cells at 0,0.5,1,2,4 hours after stress application.RESULTS AND CONCLUSION:(1) After 1-3 days of culture,0.023,0.046,0.5 mmol/L aspirin promoted the proliferation of MG-63 cells,while after 1-7 days of culture,1,2,4 mmol/L aspirin inhibited the proliferation of MG-63 cells.(2) Under the fluid shear stress,aspirin showed significant effects on the cell proliferation as confirmed by one-way analysis of variance (F=8.349,P=0.004),and 0.5 mmol/L aspirin inhibited the cellular proliferation of MG-63 cells.However,surface modification and stress loading time showed no significant effects on the cell proliferation (F=2.826,P=0.064;F=0.893,P=0.406).(3) Under the fluid shear stress,surface modification showed no significant effect on the cell proliferation of MG-63 cells cultured with 0.5 mmol/L aspirin (F=1.803,P=-0.171).Under the fluid shear stress,0.5 mmol/L aspirin significantly inhibited the proliferation of MG-63 cells on the glass slide (P=-0.003),while PT and SLA titanium surfaces showed on inhibitory effect on the cell proliferation (P=0.891,P=0.051).The present results demonstrate that the cell proliferation of MG-63 is related with aspirin in a concentration-dependent manner.In addition,different titanium surfaces may decrease the sensitivity of MG-63 cell proliferation to aspirin.

The study of the colorimetric characteristics of the cobalt-chrome alloys abutments covered by four different all-ceramic crowns by using dental spectrophotometer / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aims to evaluate the optical data of the different sites of the cobalt-chrome (Co-Cr) alloy abutments covered by four different all-ceramic crowns and the color difference between the crowns and target tab using a digital dental spectrophotometer.</p><p><b>METHODS</b>Ten Co-Cr alloy abutments were made and tried in four different groups of all-ceramic crowns, namely, Procera aluminia, Procera zirconia, Lava zirconia (Lava-Zir), and IPS E.max glass-ceramic lithium disilicate-reinforced monolithic. The color data of the cervical, body, and incisal sites of the samples were recorded and analyzed by dental spectrophotometer. The CIE L*, a*, b* values were again measured after veneering. The color difference between the abutments covered by all-ceramic crowns and A2 dentine shade tab was evaluated.</p><p><b>RESULTS</b>The L* and b* values of the abutments can be increased by all of the four groups of all-ceramic copings, but a* values were decreased in most groups. A statistical difference was observed among four groups. After being veneered, the L* values of all the copings declined slightly, and the values of a*, b* increased significantly. When compared with A2 dentine shade tab, the ΔE of the crowns was below 4.</p><p><b>CONCLUSION</b>Four ceramic copings were demonstrated to promote the lightness and hue of the alloy abutments effecttively. Though the colorimetric baseline of these copings was uneven, veneer porcelain can efficiently decrease the color difference between the samples and thee target.</p>

The optical property of four different all-ceramic crowns on the light color core ND1 substructure / 实用口腔医学杂志

Objective:To evaluate the optical property of the light color core substructure ND1 overlaid by 4 different all-ceramic crowns respectively and to study the color difference between the crowns and target tab.Methods:Light color(ND1)substructures were made and were respectively tried in 4 groups of all-ceramic crowns (Procera alumina,Procera zirconia,Lava zirconia and IPS E.max glass-ceramic lithium disilicate-reinforced monolithic,n =10).The CIE L*,a*,b* values of the cervical part,body and incisal site of the samples were recorded and analyzed by a spectrophotometer before and after veneer.The color difference between the all-ceramic crowns and target A2 dentin tab was evaluated.The spectral integral transmittance of the 4 copings was measured by a spectrophotometer.Results:The L* values of the abutments was increased by all-ceramic copings of the 4 groups,a*values were decreased but b* changed inconsistently.After veneer,the L* values of all the copings declined significantly,the values of a* and b* increased.When compared with A2 tab,the ΔE of the crowns was 1.27 ~4.17.The mean value range of the transmittance of the coping was:E.max-LT A2 >Pro-Alu >Lava-Zir >Pro-Zir.Conclusion:The lightness,hue and light transmittance of the 4 ceramic copings are different.After veneer with A2 dentine,the difference of the lightness is declined but the difference of the hue is signifi-cant.The color difference of the Lava-Zir and IPS E.max is clinically unacceptable.

RGD peptide-modified chitosan as a gene carrier of implant surface / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.</p><p><b>METHODS</b>An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS. An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed. The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer. The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy. The grafting effect of this complex on the titanium surface was detected by EB staining.</p><p><b>RESULTS</b>CS and RGD peptides were coupled by an amide bond. The RGD-CS/pDNA complex was completely composited at N/P > or = 2. Atomic force microscopy results showed that the morphology of this complex was mainly spherical. EB staining experiments showed that this complex was successfully grafted on the titanium plate.</p><p><b>CONCLUSION</b>RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.</p>

Construction of human bone morphogenetic protein 2 and histidine eukaryotic expression plasmid and synthesis of chitosan/pIRES2-EGFP-hBMP2-His nanoparticles / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To clone and construct a eukaryotic expression vector of human bone morphogenetic protein (BMP) 2 and histidine in vitro and synthesize chitosan (CS)/pIRES2-EGFP-hBMP2-His nanoparticles.</p><p><b>METHODS</b>pMD18T-hBMP2-His was digested by EcoR I and BamH I to obtain the hBMP2-His gene, which was inserted into pIRES2-EGFP to form pIRES2-EGFP-hBMP2-His. Afterward, CS, which exhibited five different molecular weights and deacetylation degrees, was complexed with pIRES2-EGFP-hBMP2-His to form CS/pIRES2-EGFP-hBMP2-His nanoparticles; in this procedure, a desolvent method was used at different N/P ratios (amino in CS to phospho in plasmid DNA). The gene-encapsulating ability of CS was evaluated by agarose gel electrophoresis and fluorescence spectrophotometry; size, distribution, and potential were analyzed using a ZetaPALS analyzer. The shape of the nanoparticles was observed under an atomic force microscope.</p><p><b>RESULTS</b>1) pIRES2-EGFP-hBMP2-His was constructed after the cloned hBMP2-His gene was confirmed by sequencing. 2) CS/pIRES2-EGFP-hBMP2-His nanoparticles were synthesized and pIRES2-EGFP-hBMP2-His was packaged by CS. 3) CS/pIRES2-EGFP-hBMP2-His nanoparticles were globular with an average size of 111.7 nm to 3,214.2 nm and an average zeta-potential of 4.93 mV to 16.79 mV.</p><p><b>CONCLUSION</b>CS/pIRES2-EGFP-hBMP2-His nanospheres are successfully synthesized.</p>

Microstructure analysis of the machinable infiltrated ceramic fracture surface / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To compare the fracture surface of the machinable infiltrated ceramic (MIC) prepared in different conditions, and to relate its strength and toughness to the differences in their microstructures.</p><p><b>METHODS</b>The Al2O3 matrix were prepared, infiltrated by the machinable glass and crystallized. Based on sintering and infiltrating time and conditions, the specimens were divided into three groups: Perfect group, insufficiently infiltrated group, mal-sintered substrate group. Specimens were fabricated, three point flexural strength and elastic modulus were tested, and the microstructure of the MIC fracture surfaces was analyzed by means of scanning electron micrograph (SEM) observation.</p><p><b>RESULTS</b>The strength of the insufficiently infiltrated group and mal-sintered substrate group were lower than that of the perfect group, and faults in the microstructure of these two groups also proved this. The foreland of glass infiltrating in the Al2O3 and the interface of glass and complex demonstrated that the Al2O3 surface was smoother than the complex and there was a big crack in the glass but not in the complex which proved the crack resistibility of glass and Al2O3 were smaller than that of the complex (MIC).</p><p><b>CONCLUSION</b>Correct preparing process could make the glass infiltrate into properly sintered Al2O3 sufficiently which ensured best mechanical properties of the complex.</p>

Comparisons of three mini-incision surgery in thymectomy for myasthenia gravis / 中国医师进修杂志

Objective To investigate the best operative approach in the treatment of myasthenia gravis (MG) by comparing surgical effects of median sternotomy, trans-sternal surgery and video-assisted thoracoscope surgery (VATS). Methods One hundred and nine patients who received thymectomy for MG were divided into median sternotomy group,trans-sternal group and VATS group according to the way of operative incision. The clinical features, such as operative time, operative blood loss, postoperative drainage volume and drainage time, postoperative hospitalization time, postoperative pulmonary infection, incisional infection, MG crisis, postoperative chronic pain and upper limb's movement disorder were retrospectively analyzed to evaluate the effect of the surgery. Results The operative blood loss, postoperative drainage volume and drainage time,and incisional infection in VATS group [(77.5 ± 18.0) ml, ( 100.8 ± 11.8) ml,( 2.3 ± 0.5 ) d, 0 ] were superior to those in trans-sternal group[ (97.4 ± 14.3 ) ml, ( 175.8 ± 18.3 ) ml, ( 3.1 ±0.7 ) d, 6.2% ( 2/32 ) ] and median stemotomy group [ ( 130.1 ± 24.0) ml, ( 379.0 ± 45.6 ) ml, (4.2 ± 0.6) d,13.8% (9/65)] (P ＜0.05),and median sternotomy group was the worst. The incidence of postoperative pulmonary infection, MG crisis, upper limb's movement disorder and postoperative hospitalization time in VATS group were superior to those in trans-sternal group and median sternotomy group (P ＜0.05).Conclusions Three mini-incision surgical approaches are safe, feasible and effective. VATS is more advantageous and is worth popularizing in large and medium-sized hospitals. Trans-sternal surgery is the better choice for primary hospitals without advanced technology and equipments.

Arithmetic for subtracting power line interference based on estimating sinusoidal parameters / 生物医学工程学杂志

Electrocardiogram (ECG) signals are often contaminated by residual power-line interference. In order to filter the noise and not to distort signal too much, certain types of digital notch filters need a narrow frequency band, but such a band leads to ineffective filtering in the case of frequency deviation of the interference. In this paper, we propose a novel arithmetic. Firstly, sinusoidal parameters (frequency and phase) are estimated from the sinusoidal values in the linear segment of ECG. Secondly, a sinusoid function in linear segment is determined by the estimated sinusoidal parameters. Thirdly, the interference values in the nonlinear segment closest to the linear segment are calculated by the sinusoid function. Lastly, we subtract the corresponding interference value from the real value. The experiment results show that, in comparison with other arithmetic, the novel arithmetic can more effectively remove the power-line interference from ECG signal.

Effects of different titanium-treated surfaces on rat's osteoblast function and cell cycle / 生物医学工程学杂志

This study is designed to compare the distribution, function and cell cycle of rat osteoblast cultured on sandblasting and acid-alkali base titanium surface versus that cultured on carbonated hydroxyapatite titanium surface; the aim is to probe the influences of different surfaces on the function of osteoblasts. SD rat's osteoblasts were primary-cultured, passaged to the 4th generation and then cultured on the two kinds of surfaces. The cellular modality was observed using an inverted microscopy analyzing system. Proliferation was examined by MTT chromatometry and depicted by growth curve. Expression of alkaline phosphatase (ALP) was detected by Enzyme-Linked Immunosorbent Assay. The cell apoptosis and proliferation were examined by flow cytometry. The proliferation curve and index of the osteoblasts on the two kinds of surfaces were almost the same. However, on the carbonated hydroxyapatite group, the level of alkaline phosphatase expression of osteoblasts was higher and kept longer than the other one. In conclusion, the elements on the titanium surface influence the cell function, and the surface with rich PO4(3-) and Ga2+ can enhance the mineralization function of rat osteoblast.

Influence factors on accuracy of CT attenuation measurement of non-calcified coronary plaque:preliminary ex vivo study / 中华放射学杂志

Objective To assess the effect of intracoronary attenuation,slice thickness,and window setting on CT attenuation measurement of non -calcified coronary plaque in ex vivo specimen.Methods Twenty adult cadaver heart specimens were used.Five solutions of contrast medium diluted with saline were prepared as follows:1/200,1/50,1/40,1/30,and 1/20.CT scans were performed after intracoronary iniection of each solution.The solution of 1/200 was used to measure baseline values of plaques.and the other four solutions were used to imitate the different intracoronary attenuation.The plaque attenuations were measured with region of interest under four intracoronary attenuations,two slice thickness (0.625 mm and 1.250 mm),and two window settings(WW 1000 HU,WL 150 HU and wW 500 HU,WL 200 HU),respectively.The result was compared with factor analysis and partial correlation analysis.Results Twenty four plaques met the study criteria.The baseline CT attenuation of plaques under two window settings were(23.90±6.87)HU(setting 1)and(29.00±7.26)HU(setting 2)respectively.Intracoronary attenuation and window settings had significant effect on CT attenuation measurement of non-calcified plaques(F=45.674 and 79.094,P＜0.01),whereas slice thickness of 0.625 mm and 1.250 mm showed no significant effect on CT attenuation measurement(F=1.033,P＞0.05).Comparing with baseline vslues.plaque attenuations on 1/40 and 1/30 solutions that can imitate lumen attenuation in clinical practice were increased.Conclusions Intraeoronary attenuation and window settings had significant effeet on CT attenuation measurement of non-calcified plaques,while slice thickness of 0.625 mm and 1.250 mm had no significant effect.Plaque attenuation would increase with the increase of intracoronary attenuation.

The effects of the upper removable partial denture on the consonants acoustic characteristics / 实用口腔医学杂志

Objective:To analyzise the upper removable partial denture for Kennedy Ⅰdentition defect how to affects the consonants acoustic characteristics. Methods: Before and after restoration use the computer speech lab(CSL) to analyze 12 patients' acoustic characteristics. Then at 1, 2, 4 and 8 weeks, to compare each patient different time's area of energy concentration, the first area of energy concentration band width of consonants /s/,/sh/,/j/; the locus of /t/. Results:There was significant difference between the area of energy concentration and the first area of energy concentration band width in /s/ of the groups before restoration and at the beginning and after one week(P0.05) in other consonants groups.Conclusion: The main area which affects the acoustic characteristics is the anterior part of the anterior palate plate of the removable partial denture. From the acoustics point of view, to lay the major connector on the upper gingival, the median line area are not suitable, while the posterior part of the hard palate fitting to lay it.

The effect of ceramometal bridge on the local oral flora / 实用口腔医学杂志

Objective:To investigate the effect of the ceramometal b ridge on the local gingival groove flora.Methods: Classi cal bacterial incubation and identification were used to study the changes of th e local gingival groove flora in 6 patients wearing ceramometal bridge for 1 wee k to 3 months. Results: 3 months after the prosthetic procedure the CFU o f P.melaninogenica, Gram-positive bacilli cmainly Actinomyces and the t otal bacteria were significantly increased (P

Study of crown and framework fitness of DA9-4 alloys / 实用口腔医学杂志

0.05 ). And the fitness values(?m) of the crown of DA9-4 alloy were:the margin gap of the crown 71.3 ?8.34, the shoulder gap 53.4?4.83, the axial wall gap 41.85?8.08, the occlusal lift amount 55.2?9.21.Conclusion:DA9-4 alloy has good fitness and can meet the require of clinic.