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Objective:To study the correlation between the occurrence of diabetes mellitus complicated with erectile dysfunction (DMED) and serum fibroblast growth factor-21 (FGF21) level, analyze the role of FGF21 in the occurrence of DMED, and explore its predictive and diagnostic value for DMED.Methods:61 male patients with type 2 diabetes in Tai′an Central Hospital from January 2017 to June 2018 were selected as subjects. They were divided into 2 groups: DMED group ( n=30) and type 2 diabetes mellitus group (DM group, n=31). And 32 healthy persons were selected as control group. The general data of all subjects were collected, physical examination, genital examination and blood sampling were carried out for biochemical analysis. The erectile function was measured by International Erectile Function Index-5 (IIEF-5) questionnaire, and serum FGF21 level was detected by automatic biochemical analyzer. The correlation between IIEF-5 score with FGF21 and other indicators were analyzed by Spearman correlation analysis. Multiple linear regression analysis was used to analyze the related risk factors affecting DMED. Receiver operating characteristic (ROC) curve was used to analyze the predictive value of FGF21 level for DMED. Results:(1) The serum level of FGF21 in the DMED group, DM group, control group were (112.5±9.2)pg/ml, (103.9±6.2)pg/ml and (91.9±3.9)pg/ml respectively, with significant differences (all P<0.05). (2) Spearman correlation analysis showed that IIEF-5 score was negatively correlated with FGF21, age, smoking, drinking, systolic blood pressure, diastolic blood pressure, glycated hemoglobin A1c (HbA 1c), fasting plasma glucose (FPG), total cholesterol (TC), total glyceride (TG), low density lipoprotein cholesterol (LDL-C), fasting insulin (FINS), homeostasis model insulin resistance index (HOMA-IR), and positively correlated with high density lipoprotein cholesterol (HDL-C) and testosterone( all P<0.05). The serum FGF21 level was positively correlated with smoking, drinking, systolic blood pressure, diastolic blood pressure, HbA 1c, FPG, TC, TG, LDL-C, FINS, HOMA-IR, and negatively correlated with HDL-C and testosterone ( P<0.05). (3) Multiple linear regression analysis showed that FGF21, HbA 1c, HOMA-IR and IIEF-5 were independent risk factors for erectile function (all P<0.05). (4) According to ROC curve, when the optimal cutoff value of FGF21 level was 102.75 pg/ml, the predictive sensitivity and specificity for DMED were 0.867 and 0.714, respectively. Conclusions:Serum FGF21 level is closely related to the occurrence of diabetes mellitus and DMED, which is an independent risk factor of DMED.
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【Objective】 To establish a dry fluorescent luminescence method for the detection of antibodies to hepatitis C virus (HCV) and evaluate its clinical application. 【Methods】 Anti-HCV antibody was detected by double-antigen sandwich dry fluorescent luminescence method established using multi-epitope chimeric antigen. The established method was used to detect national reference samples(positive 20, negative 20), and a total of 349 clinical samples, including 108 HCV patients, 36 patients with other diseases and 205 healthy individuals, which were tested in parallel with enzyme-linked immunoassay (ELISA) to evaluate the performance of the established method. 【Results】 The concordance rate of positive and negative(each 20) reference samples were both 100% (20/20), and the CV of precision reference sample was 9.16%, which met the requirements of national reference samples. In clinical performance evaluation, the AUC value was 0.984, and the sensitivity and specificity of the dry fluorescent luminescence method were 96.30% (104/108) and 96.27% (233/241). The overall concordance rate between dry fluorescent luminescence method and ELISA was 97.71% (341/349) (Kappa=0.952). 【Conclusion】 The dry fluorescence luminescence method of HCV antibody is simple and rapid, with high sensitivity and high specificity, and can be used in clinical application.
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Abstract@#During the yellow fever epidemic in Angola in 2016, cases of yellow fever were reported in China for the first time. The 11 cases, all Chinese nationals returning from Angola, were identified in March and April 2016, one to two weeks after the peak of the Angolan epidemic. One patient died; the other 10 cases recovered after treatment. This paper reviews the epidemiological characteristics of the 11 yellow fever cases imported into China. It examines case detection and disease control and surveillance, and presents recommendations for further action to prevent additional importation of yellow fever into China.
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Objective To evaluate the clinical and technical effects of self-expanding VENA stent in the treatment of iliofemoral vein obstruction.Methods The clinical data of 58 patients(61 limbs) with symptomatic iliofemoral vein obstructive disease treated by VENA stent from February 2017 to June 2018 were collected and analyzed.The patency of the vein was assessed by the results of intraoperative angiography and postoperative symptoms relief changes in leg circumference.Follow up included relief of symptoms,Doppler ultrasound.Results A total of 63 VENA stents (43 in the left limb and 20 in the right limb) were implanted,sizes ranging from 12 mm to 16 mm,surgical technique success rate was 100％.The median follow-up time was 8.6 months.The primary patency rate of one month,three months,six months and 12 months was 96％,94％,92％ and 92％,respectively.Leg circumference fall down from(48 ±0.4) cm to (37 ± 0.3) cm (P ＜ 0.05).Conclusion Self-expanding nitinol stent implantation is a safe and effective treatment method for symptomatic iliofemoral vein obstruction disease.
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Objective To try different concentrations of nicotine promotes the apoptosis of human umbilical vein en-dothelial cells and its mechanism .Methods Human umbilical vein endothelial cells were cultured .According to the concentration of nicotine , grouped as 10 -6 , 10 -7, 10 -8 mol/L, and control.After 24 h of nicotine treat-ment, proliferation/activity were tested by CCK-8 assay;Annexin V-FITC fluorescence staining detects the apoptot-ic cells.Western blot detects the expression of protein Bax , Bcl-2 and PARP-1, to study the mechanism of apopto-sis.Results Compared with control group , in 10 -6 ,10 -7 mol/L groups the proliferation/activity of HUVECs de-creased ( P<0.05 ) .And the number of apoptotic cells increased ( P<0.01 ) .The target proteins expression of 10 -6、10 -7 mol/L groups were changed as follow: Bax increased ( P <0.01 ) , Bcl-2 decreased ( P <0.01 ) and PARP-1 increased ( P<0.01 ) .Conclusions Nicotine may promote the apoptosis of vascular endothelial cells , which accelerates the development of atherosclerotic disease .
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Objective:To establish the quality standard for Herba Euphorbia esulae. Methods:A TLC method was used to identify Herba Euphorbia esulae. An HPLC method was used to determine the content of quercetin with the following conditions: an AlltimaTM C18column(150 mm ×4.6 mm,5 μm) was eluted with the mobile phase of acetonitrile-0.4％ phosphoric acid(30 :70), the flow rate was 1. 0 ml·min-1 , the detection wavelength was 360 nm and the column temperature was 35℃. Results:The characteristic spots of samples were the same as those of the standard samples. The linear range of quercetin was 3.194-102.208 μg·ml-1(r=0.9999) and the average recovery was 99. 0％ (RSD=1. 68％, n=6). Conclusion:The method is quick, simple and accurate, which can be used for the quality control of Herba Euphorbia esulae.
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Objective: To establish an HPLC-ELSD method for the content determination of rosolic acid in Radix Rubi.Methods: The ELSD-HPLC content determination of rosolic acid was set up using a Vision HT C 18 HL column(250 mm× 4.6 mm ,5 μm), the mobile phase was methanol-0.1% trifluoroacetic acid with a flow rate of 0.8 ml·min-1 , the column temperature was 35℃, the temperature of drift tube heater was 90 ℃ and the gas was with the flow rate of 2.3 L ·min-1 .Results: The linear range of rosolic acid was 0.155-4.346 μg (r=0.999 9).The average recovery was 100.4% with RSD of 1.88% (n =6).Conclusion: The method is simple and accurate.It can be used for the quality control of Radix Rubi.
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Objective:To study the preventive and therapeutic effects of Jiechangyan Qixiao granule ( JQX) in the rats with ulcer-ative colitis ( UC) induced by dextran sulfate sodium .Methods:The UC model was induced by drinking dextran sulfate sodium ( DSS, 4%) freely in Wistar rats weighting 180-220g.Guben Yichang tablets and sulfasalazine was used as the standard drugs for the compari -son.After 7-day intragastric administration of Jiechangyan Qixiao granules at the dose of 2.70, 1.35 and 0.68 g· kg-1 · d-1 , the se-rum levels of malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), interleukin -6(IL-6)and tumor necrosis factor-α(TNF-α), and the protein expression of myeloperoxidase (MPO) and intercellular adhesion molecule (ICAM-1)in colon and nuclear factor(NF-kBp65) were detected.Results:Compared with the model group, the granule at high and medium dose could sig-nificantly increase the activity of SOD in blood and decrease the contents of MDA , TNF-αand IL-6 (P<0.05 or P<0.01).The granule could also notably decrease the MOP activity in colonic mucous of UC rats (P<0.05 or P<0.01), and the contents of NF-kBp65 and ICAM-1 in the inflammation reaction(P<0.01).Conclusion: JQX shows promising efficacy in the treatment of UC rats induced by dextran sulfate sodium .
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Objective:To establish an HPLC-ELSD method for the determination of mahuannin A in ephedrae radix et rhizoma. Methods:The content of mahuannin A was determined by an HPLC-ELSD method on an Alltima TM C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was acetonitrile-water (28∶ 72) with a flow rate of 0. 7 ml·min-1, and the column temperature was 30℃. The temperature of drift tube heater was 105℃ and the flow rate of carrier gas was 2. 8 L·min-1 . Results:The linear range of mahua-nnin A was 42. 56-383. 04 μg·ml-1(r=0. 999 8). The average recovery and RSD was 99. 9% and 1. 96%(n=6), respectively. Conclusion:The method is simple and the result is accurate. It can be used for the quality control of ephedrae radix et rhizaoma.
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Thladiantha dubia Bunge is a traditional Chinese medicine in Manzu region applied in the treatment of pain in waist and leg, or strain in lumbar without adverse reaction. By referring to the relative literatures on Thladiantha dubia Bunge from home and abroad, the study progress in the chemical constituents and pharmacological actions of Thladiantha dubia Bunge in the recent 30 years were reviewed to lay foundation for the reasonable exploitation and utilization of Thladiantha dubia Bunge.
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Objective:To optimize the extraction process of the total flavonoids from Ephedrae Radix Et Rhizoma. Methods:The purification method of the total flavonoids from Ephedrae Radix Et Rhizoma was optimized with the yield and content of the total fla-vonoids as the indices. Based on the above research, the process parameters were optimized by an orthogonal test. Results:The opti-mum purification conditions were as follows:the volume fraction of ethanol was 50%, the stirring extraction time was 20 min, and the liquid-solid ratio was 8∶ 1(ml·g-1). Conclusion:The optimum purification technology is simple and reproducible, and suitable for the industrial production.
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Objective:To investigate the therapeutic effects of Gengnian Anshu granule on the endocrine system of menopausal rats. Methods:The rats aged 13-15 months received intragastrical treatment with Gengnian Anshu granule at the doses of 1. 9,0. 95 and 0. 48 g·kg - 1 ·d - 1 for 6 weeks. At the end of treatment,serum and hypothalamic hormone levels were detected,and the organ indices including uterus and ovaries were determined. Results:In the menopausal rats,Gengnian Anshu granule could significantly in-crease the serum estradiol(E2 )(P < 0. 05 or P < 0. 01),decrease serum follicle-stimulating hormone(FSH)and luteotrophic hor-mone(LH)(P < 0. 05 or P < 0. 01),increase norepinephrine(NE),dopamine(DA),5-hydroxytryptamine(5-HT)and β-endor-phine(β-EP)(P < 0. 05),increase the number of cells positive for estrogen receptor(ER)protein expression in hypothalamus(P <0. 05 or P < 0. 01),and decrease the number of cells positive for gonadotropin-releasing hormone(GnRH)protein expression(P <0. 01). Conclusion:With a multi-target action mechanism and an integral regulatory role,Gengnian Anshu granule can provide favor-able regulation against endocrine disorder in menopausal rats.
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Objective To determine the expression level of microRNA-25 in animal models of diabetic nephropathy and human renal tubular epithelial cells (HK-2) cultured in different conditions, and to explore its regulating effect on the fibrosis in diabetic nephropathy. Methods The expression of microRNA-25 was detected by real-time PCR. The downstream target protein of microRNA-25 was verified by bioinformatic prediction, transient transfection of cells and Western blotting. Results MicroRNA-25 was down-regulated in animal models of diabetic nephropathy and HK-2 cells which were cultured in high-glucose medium (P<0. 01). MAP2K4 might be the downstream target protein of microRNA-25. Overexpression of microRNA-25 reduced the protein expression of MAP2K4 and α-SMA (P<0. 01). Conclusion MicroRNA-25 inhibits the fibrosis in diabetic nephropathy by regulating the expression of MAP2K4.
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Objective:To describe the current situation of IHR ( 2005 ) public health emergency core capacity at the provincial, city, district or county level for health departments in China, discover gaps in the implementation of IHR(2005) public health emergency core capacity, and thereby provide reference for the construction of IHR(2005) public health emergency core capacity. Methods: A survey was conducted from 211 questionnaires of IHR (2005) public health emergency core capacity, which included 7 provinces, 64 cities, and 140 counties ( districts) in the health departments. Results:The indicator of IHR (2005) public health emergency core capacity is suitable for the conditions in China;there is a lack of institution building in China concerning IHR (2005) public health emergency core capacity;there is a decreasing trend at the provincial, city and county ( district) level. Conclusion:Recommen-dations were proposed to strengthen China’s institution building of IHR (2005) public health emergency core capaci-ty, focus on strengthening construction of county (district) level IHR (2005) public health emergency core capacity, strengthen the weak links of the IHR (2005) public health emergency core capacity index, strengthen China’s appli-cability research concerning IHR (2005) public health emergency core capacity.
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Objective:To investigate the clinical pharmaceutical care methods and promote rational drug use. Methods:The diag-nosis and treatment process of one patient with chronic obstructive pulmonary disease were used as the examples, the pharmacists par-ticipated in selecting drugs, adjusting dosage and making individual regimen to carry out the pharmaceutical care in the whole treatment process, including observing curative effect, monitoring adverse reactions, implementing patient education,nutritional support, immu-notherapy and the effect evaluation. Results:Clinical pharmacists together with clinicians developed the treatment regimens for the pa-tient with the full implementation of pharmaceutical care, the effect was promising, and the patient recovered and discharged from hos-pital. Conclusion:Clinical pharmacists should implement pharmaceutical care for patients with chronic obstructive pulmonary disease in the whole treatment process, which can reduce drug adverse reactions and interactions effectively, and play an important role in ra-tional and safe drug use.