RESUMEN
The semen preparation medium that yields good semen quality is one among various important factors in the process of infertility treatment. The objective of this study was to compare the semen analysis parameters before and after preparation with 3 semen preparation media i.e. PureSperm® (Nidacon, Gothenburg, Sweden), Sil-Select Plus™ (Fertipro, Beernem, Belgium) and SpermGrad™ (Vitrolife, Gothenburg, Sweden) for enhancement of good semen quality.The total of 28 males of infertile couples attending Fertility Clinic of Thammasat University Hospital during the year 2007 were studied. Their semen (pre-washed) were analyzed for semen analysis parameters according to WHO¹ and Kruger’ strict criteria² and then equally divided into 3 aliquots and processed with 3 semen preparation media followed by semen analysis once again (post-washed). The sperm concentration, motility, progressive motile concentration (PMC), morphology, DNA damage and protamine deficiency of before and after preparation were compared by paired t-test with significance at P \< 0.05.The mean percentage of sperm motility, PMC, rapid motility and normal morphology were significantly increased where the mean percentage of slow motility, sperm head defect, sperm concentration and protamine deficiency were significantly decreased after preparation with all 3 media. The DNA damaged sperm was significantly decreased in the post-washed with PureSperm® and Sil-Select Plus™ but significantly increased with SpermGrad™.From the results obtained in this study, it could be concluded that PureSperm® may be suitable for both IUI and IVF techniques, since it yielded the best motile sperm and it may be also good for ICSI because it gave low percentage of DNA damaged sperm. Sil-Select Plus™ is probably best for ICSI since it gave lowest percentage of DNA damage sperm, whereas SpermGrad™ had highest percentage of DNA damage, thus, it is surely not suitable for ICSI procedure. Anyhow, SpermGrad™ may be moderately good for IUI since it had high percentage of motility and of normal morphology with lowest percentage of head defect but percentage of abnormal embryo may be increased.
RESUMEN
Objective: To isolate and characterize mesenchymal stem cells (MSCs) from peripheral blood and G-CSF mobilized peripheral blood. Methods: Mononuclear cells (MNCs) were isolated from peripheral blood, G-CSF mobilized peripheral blood and bone marrow using gradient centrifugation. The numbers of MSCs in these three sources were quantified using flow cytometry. The isolated MNCs were then cultured to generate MSCs. The MSCs generated from those three sources were studied in term of the MSC marker (CD73, CD90, CD105 and CD106) expression, the ability to generate colony (CFU-F) in culture and the ability to differentiate toward osteocyte and adipocyte-lineages. Results: The percentage of cells that expressed CD90 in fresh MNC populations isolated from bone marrow (BM-MNCs), peripheral blood (PB-MNCs) and mobilized peripheral blood (MPB-MNCs) is 1.94, 2.1 and 0.05 respectively, while the expression of CD73 in BM-MNCs, PB-MNCs and MPB-MNCs is 5.2, 15.2 and 7.8 respectively. The percentage of cells that expressed CD105 in BM-MNCs, PB-MNCs and MPB-MNCs is 5.3, 4.1 and 2.75, respectively while the expression of CD106 in those three populations is 2.82, 2.36 and 4.5 respectively. The ability of BM-MNCs, PB-MNCs and MPB-MNCs to generate colony in culture (CFU-F) is 67, 30 and 48 colonies per 10⁶ plating MNCs, respectively. After culture for three passages, more than 64% of BM-MNCs, PB-MNCs and MPB-MNCs homogeneously expressed CD73, CD90 and CD105. In contrast, the expression of CD45 (marker of hematopoietic cells) in those populations is negative. In addition, the bone marrow-derived MSCs also have an ability to differentiate toward osteocyte and adipocyte-lineages. Conclusion: We have successfully isolated and characterized MSCs from both peripheral blood and G-CSF mobilized peripheral blood. Those MSCs expressed several MSC markers, including CD73, CD90, CD105 and CD106, and able to generate colonies in culture in a manner similar to those of BM-MSCs. Our results suggest that these PB-MSCs and MPB-MSCs might be used as an alternative source for the clinical treatment in the future.
RESUMEN
Objective: To compare semen analysis parameters between bacteriospermia and non-bacteriospermia specimens in men attending at infertile clinic.Methods: A total of one hundred men who attending an infertility clinic between 1st September 2006 -31st January 2007 at Thammasat hospital was enrolled into the study. After soap and water skin preparation, all men gave semen specimens for culture and semen analysis by Computer-Assisted Sperm Analysis (CASA). The main outcome measures were presence or absence of bacteriospermia, the specific bacterial isolate and semen analysis parameters.Results: The incidence of bacteriospermia was 14%. Staphylococcus species were the most common organisms following by Streptococcus species, Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa, respectively. There was no significant difference in semen analysis parameters especially leukocytospermia between bacteriospermia and non-bacteriospermia specimens (P \> 0.05).Conclusions: Bacteriospermia does not impact to semen analysis parameters especially leukocytospermia.