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Objective To investigate the efficacy and safety of clopidogrel hydrogen sulfate plus nimodipine in treating transient cerebral ischemic attack.Methods A total of 159 outpatients and inpatients with transient cerebral ischemic attack in the neurosurgery department and the neurology outpatient department of our hospital from January 1,2012 to September 1,2016 were divided into 3 groups.The group A(43 cases) orally took clopidogrel hydrogen sulfate plus aspirin;the group B (51 cases) treated with oral clopidogrel hydrogen sulfate plus nimodipine;the group C(65 cases) was treated with clopidogrel hydrogen sulfate plus nimotop.The curative effects were observed in 3 groups.The differences of PT.APTT,FIB and PLT in 3 groups were compared between before and after treatment.Results The total effective rate of the group A and C was significantly better than that of the group B(P0.05);the effect of the group B and group C on coagulation was superior to that of the group A(P<0.05).The three group had no severe bleeding events(cerebral bleeding,etc.),the difference was statistically significant.Conclusion Clopidogrel hydrogen sulfate combined with nimotop can significantly reduce the frequency of transient cerebral ischemic attack,has little influence on the coagulation indicators,and is safe and effective.
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Objective:To investigate the rapid establishment of nude mice-C6 glioma model and its application feasibility in research experiments.Methods:C6 mice glioma cell-suspension was inolulated to the male and female nude mice subcutaneously.The period for tumor tissue to grow out was detected and the histological and immunohistochemical features of tumor were observed.Tumor tissue was used to do allograft transplant for the male and female nude subcutaneously.The sprouted time of subcutaneous transplant tumor in nude mice was detected.Pathological and immunohistochemical features of tumor were studied.Results:(1)Success rates of both two methods were 100%,tumor grew in good condition,and the histological and immunohistochemical examination results of tumor were consistent with glioma cell morphology;(2)The method of cell inoculation to establish model took a long time.Tumor growing speeds in male and female nude mice were different and the difference had statistical significance(P0.05) Conclusion:(1)Tumor tissue transplant can establish subcutaneous xenograft model quickly,and to provide the reliable animal model for studying of glioma、biological characteristics and gene therapy;(2)Tumor tissue allograft can shorten the establishment time of animal models saving the cost of scientific research.