Application and study of DNATyper~(TM)15 kits in forensic medicine / 中国法医学杂志

Objective To study the application of DNATyperTM15 kit in forensic medicine. MethodsCollecting 60 kinds of different testing material totally 8137 samples in 34 labs, Using 5 kinds of different methods to extract DNA, Using the DNATyperTM15 kit to amplify and inspect, Comparing with the other two imported kits of the same kind. ResultsDNATyperTM15 kit is suitable to the different extract methods, testing material, instrument of the amplification and inspection, operation environment and the operator. ConclusionDNATyperTM15 kit can be applied in forensic test and identification.

The nomenclature principle of loci and alleles of Y-STRs / 中国法医学杂志

In recent years, along with the in-depth research of Y-STRs, a lot of new loci are found and applied in forensic medicines. To solve some questions, the International Society of Forensic Genetics have published two guidance and recommendations successively concerning the Y-STRs in 2001 and 2005, which clarify contents regarding the term, the nomenclature principle of loci and alleles and the population genetics et al of Y-STRs. This report mainly describes the nomenclature principle of loci and alleles of Y-STRs.

Validation of the DNATyper~(TM)15 PCR amplification kit / 中国法医学杂志

Objective Developmental validation studies were designed according to the standards of forensic DNA community on DNATyperTM15 kit,which simultaneously amplifies 14 STR loci(D6S1043、D21S11、D7S820、CSF1PO、D2S1338、D3S1358、D13S317、D8S1179、D16S539、Penta E、D5S818、VWA、D18S51、FGA)and amelogenin,a sex-determining locus.Methods Several key factors were tested including:Ⅰ.amount of hot-start Taq polymerase;Ⅱ.annealing temperature;Ⅲ.sensitivity;Ⅳ.reaction volume;Ⅴ.cycle number;Ⅵ.primer concentration.DNATyperTM15 was also compared with two other widely used commercial STR amplification kits,namely IdentifilerTM and PowerPlex.Results No difference in performance was observed with three lots of DNATyperTM15.Performance was not affected even after 20 times of repeated freeze-and-thaw.All three kits performed comparably in the following aspects:Ⅰ.sensitivity;Ⅱ.ability to genotype mixed samples;Ⅲ.amplification of DNA from various sample sources.DNA extraction methods(Chelex-100,magnetic beads,silica beads)did not result in any observable effect on performance with any of the three kits.Conclusion All the results demonstrated that DNATyperTM15 is suitable for both forensic DNA database work and casework.