Chemical Composition Analysis and Repellent Activity Evaluation of Compound Mosquito Repellent Essential Oil / 浙江中医药大学学报

[Objective]To analyze the chemical components of the compound essential oil for mosquito repellent,which was composed of Menthae haplocalyx Briq.,Spirodela polyrrhiza(L.)Schleid,Acorus tatarinowii Schott and Rosmarinus offiicinalis L.,and to evaluate its repellent effect.[Methods]The chemical components of the compound mosquito repellent essential oil were analyzed by gas chromatography-mass spectrometer(GC-MS),and the repellent activity of the essential oil against adult Aedes albopictus was evaluated according to the"Efficacy Test and Evaluation of Space Repellent Products(Y-tube Method)".[Results]Fifty-eight main volatile components were identified,accounting for 98.28％of the total components of essential oil,eighteen terpenes(44.99％),nine alkenes(20.27％),five esters(14.07％),eight aromatic hydrocarbons(12.00％),seven alkanes(3.48％),nine alcohols(2.78％),two ketones(0.69％).The percent repellency(PR)of the compound mosquito repellent essential oil against Aedes albopictus was more than 90％on average.[Conclusion]There were many chemical components in the compound mosquito repellent essential oil.There may be a variety of chemical components such as menthol,menthone,α-pinene,etc,which had a repellent effect on Aedes albopictus,and the average repellent effect reached the A level.

Combination of berberine and ciprofloxacin reduces multi-resistant Salmonella strain biofilm formation by depressing mRNA expressions of luxS, rpoE, and ompR

Bacterial biofilms have been demonstrated to be closely related to clinical infections and contribute to drug resistance. Berberine, which is the main component of Coptis chinensis, has been reported to have efficient antibacterial activity. This study aimed to investigate the potential effect of a combination of berberine with ciprofloxacin (CIP) to inhibit Salmonella biofilm formation and its effect on expressions of related genes (rpoE, luxS, and ompR). The fractional inhibitory concentration (FIC) index of the combination of berberine with CIP is 0.75 showing a synergistic antibacterial effect. The biofilm's adhesion rate and growth curve showed that the multi-resistant Salmonella strain had the potential to form a biofilm relative to that of strain CVCC528, and the antibiofilm effects were in a dose-dependent manner. Biofilm microstructures were rarely observed at 1/2 × MIC/FIC concentrations (MIC, minimal inhibition concentration), and the combination had a stronger antibiofilm effect than each of the antimicrobial agents used alone at 1/4 × FIC concentration. LuxS, rpoE, and ompR mRNA expressions were significantly repressed (p < 0.01) at 1/2 × MIC/FIC concentrations, and the berberine and CIP combination repressed mRNA expressions more strongly at the 1/4 × FIC concentration. The results indicate that the combination of berberine and CIP has a synergistic effect and is effective in inhibiting Salmonella biofilm formation via repression of luxS, rpoE, and ompR mRNA expressions.

Discussion on the Cultivation of Medical Students' Medical Humanistic Spirit from the Perspective of Ideological and Political Education / 中国医学伦理学

The ideological and political education of medical students is an indispensable part of medical education.Strengthening the ideological and political education of medical students and cultivating the medical humanistic spirit will contribute to the benign development of the medical industry in the long run.Starting from ideological and political education of medical students,this paper explored the training path of medical students' medical humanistic spirit from the perspective of ideological and political education in the aspects of deepening the content of medical humanistic education,expanding the form of medical humanistic education,perfecting the evaluation of medical humanistic education,and integrating ideological and political education with clinical practice.