RESUMEN
A cepa MN do HIV-1 e conhecida por ser mais prevalente no Brasil, a cepa BRU e mais prevalente na Europa e a cepa NDK na Africa. Tem sido sugerido na literatura que diferentes cepas devam ser incluidas em uma mesma vacina contra o HIV-1. Com o intuito de se contribuir para o desenvolvimento de uma vacina universal, a ocorrencia de anticorpos (Ac) contra tres cepas do HIV-1 (MN, BRU, NDK) foram determinadas em amostras de soros de 85 pacientes voluntarios, adultos, soropositivos para o HIV-1, atendidos em um Hospital Universitario. Cem unidades infectantes para cultura de tecido (TCIU) dos virus reagiram com diluicoes seriadas de soro (2x) e celulas MT4 adicionadas a uma concentracao final de 0,3 x 10 elevado a sexta potencia celulas/ml...
Asunto(s)
Humanos , Masculino , Femenino , Anticuerpos Anti-VIH , Infecciones por VIH/diagnóstico , Pruebas de Neutralización/métodos , Técnicas de Cultivo , VIH-1/inmunología , Grupos de RiesgoRESUMEN
Acquired immune deficiency syndrome (AIDS) is an epidemic immunesuppresivbe disease characteristically associated with a depletion of T4 lymphocytes of the helper phenotype. A virus currently named HIV (human immune deficiency virus_ was repeatedly isolated from lymphocytes from patients with AIDS of from those atrisk for the disease. HIV is a retovirus provisionally included in the Lentiviruses subfamily because it shares properties in common with Visna-Maed virus, like ability to cause slow infection, cell fusion and other cythopathic effects in cultured cells. The AIDS virus homes to a receptor on the surface of helper T cells. Circulating antibodies to HIV can be detected in sera from AIDS patients and people in various high risk groups. Eight five percent of asymptomatic carriers harbour the virus in the presence of antibodies. Virus and antibodies are also present in patients with generalized lymphadenopathies, a state of ARC (AIDS related complexes) and in AIDS patients who suffered opportunistic infections or Kaposi sarcoma