Determination of piperine in Anar-5 tablets by high performance liquid chrotamography and method validation / Монголын Анагаах Ухаан

Introduction@#One of the main ingredients of Anar-5 tablets is Piper longium L. Piperine alkaloids are the main active ingredients of the Piper longum and have anti-inflammatory, antioxidant and gastric protection properties.In the framework of the standardization study of Anar-5 tablets, a method was developed to determine the content of piperine in highly perpormance liquid chromatography, and then it was sought to include it in the method of analysis of Anar-5 drugs.@*Goal@#Quantitative determination of piperine in Anar-5 tablets and validate the method@*Material and Methods@#The research was conducted in the Chemistry and Chemical Technology Laboratory of the Research Center of the Institute of Traditional Medicine and Technology. And Anar-5 tablets (serial number 04012020) that are produced for experimental were used in the research. The standard substance, piperine alkaloids, was purchased from Green Chemistry.Purification of HPLC (organic solvent methanol, 99.9%, distilled water) was used. The EX 1600 HP/ PUMP high-performance liquid chromatography instrument (column Arcus EP+-C18, 5µm, 4.6x250 mm) and the organic solvent filter 0.45 μm syringe filter were used. The methodology related to this research was discussed and approved at the online meeting of the Ethics Committee of the Academy of Sciences on January 26, 2021. SPSS 16 software was used to statistically program the survey results.@*Results @#According to the above method, the retention time of the standard piperine is 10.38± 0.02 minutes, and the retention time of the piperine in Anar-5 tablets is 10.42±0.033 minutes. Relative velocity deviation RSD 1.077%, accuracy 0.65446±0.0068mg, stability 0.61298±0.013mg, capture time 10.42±0.033 minutes, relative standard deviation RSD≤2%, specificity 10.35 minutes, The equation of a line constructed with a standard curve is y=43360x-33587 and the correlation coefficient R2=0.9989. The piperine content of Anar-5 tablets was determined to be 0.61298±0.013 mg. The LOD and LOQ for piperine were in 2.268 μg/ml and 6.873 μg/ml, respectively.@*Conclusion@#The content of piperine in Anar-5 tablets can be determined by the HPLC method, and the appropriate conditions for this method have been established. The HPLC method is unique, accurate, linear, and stable, and meets ICH Q2 (R1) guideline criteria.

Clarification of ancient works written by foreign researchers on mongolian pharmacology and pharmaceutical formulation / Монголын эм зүй, эм судлал

Abstract@#In recent years, the study of pharmacology and pharmaceutical formulation of traditional Mongolian medicine has been developing significantly. However, there are few research papers on Mongolian pharmacology and development of pharmaceutical formulation written by foreign researchers in ancient times. Therefore, the study was carried out to fill this “gap” and to clarify the ancient works of foreign researchers related to Mongolian pharmacology and development of pharmaceutical formulation.@*Purpose of the study@#To chronologically identify the early works of foreign researchers on the study of pharmacology and pharmaceutical formulation of traditional Mongolian medicine.@*Research method@#Hermeneutic approach, analysis of ancient textbook, and analysis and synthesis methods were used.@*Conclusion@#According to facts and eyewitnesses in the book of Zhouhou Beijifang (Emer- gency Prescriptions) by Ge Hong (261-341) of the Jin Dynasty, Jingui Yaolüe (Essential Prescriptions from the Golden Cabinet) by Chang Ji, the Northern Han Dynasty’s notes on “Xiongnu Doctors Carry Toxic Drugs”, Jāmiʿ al-Tawārīkh (Compendium of Chronicles) by Rashid-ad-Din, William de Rubruck’s “Journey to the Orient”, and book of Plano Carpini, an Italian tourist, the development of pharmacology and pharmaceuticals in traditional Mongolian medicine dates back to the Xiongnu period. Later, in the fourteenth century, it became an independent system of medical knowledge of many nations, as evidenced by the writings of Chinese, Persian, French, and Italian scholars as well as religious envoys.

ХОДООДНЫ АРХАГ ҮРЭВСЛИЙН ЭМГЭГ ЗАГВАР ҮҮСГЭЖ АНАР-5 НИЙЛМЭЛ ЖОРЫН ҮЗҮҮЛЭХ НӨЛӨӨГ СУДАЛСАН ҮР ДҮН / Шинэ санаа Шинэ нээлт

@#BACKGROUND. The Mongolian traditional medicine Anar-5 is excellent for weak digestion and helps with stomach irritation, loss of appetite, and resulting body weakness. Anar-5 blends punicagranatum, cinnamomum cassia presl, piper longum, cardamom and alpiniaofficinarum. We are establish an experimental animal of chronic gastritis to investigate the effect of traditional medicine Anar-5 on rats gastric mucosa. Methods: In this study, the protective effect preparation in sixty five healthy, male wistar rats were treated with intragastric administration of ammonia water 0.1%. To rats in three experiments for 2 week, 4 week, and 6wk, gastric tissues were examined histopathologically for atrophic changes and blood’s gastrin produced by preparation treatment. Results: After the treatment of animals blood’s gastrin was significantly different from that in control group (p<0.05), and the gastric mucosal inflammation was infiltration of inflammatory cells, decreased thickness of lamina propria. Conclusion: Treatment with preparation from Anar-5 protectived by the chronic gastritis and gastric atrophy.

Total phenolic, flavonoid, alkaloid and iridoid content and preventive effect of Lider-7-tang on lipopolysaccharide-induced acute lung injury in rats

Lider-7-tang, a medicine used for the treatment of respiratory diseases especially pneumonia and fever in Mongolian Traditional Medicine, was selected for this phytochemical and pharmacological study. The objectives of the study were to determine total biological active substances and analyze the effects of Lider-7-tang treatment in rats with acute lung injury (ALI). Quantitative determination of the total active constituents (phenolic, flavonoid, iridoid and alkaloid) of the methanol extract of Lider-7-tang was performed using Folin-Ciocalteu reagent, aluminum chloride reagent, Trim-Hill reagent, and Bromocresol green reagent, respectively. A total of fifty 8-10-week-old male Wistar rats (200-240 g) were randomized into three groups: control group, lipopolysaccharide (LPS) group (7.5 mg/kg) and LPS+Lider-7 group (90 mg/kg Lider-7-tang before LPS administration). The total content of alkaloids was 0.2±0.043%, total phenols 7.8±0.67%, flavonoids 3.12±0.206%, and iridoids 0.308±0.0095%. This study also evaluated the effects of Lider-7 on levels of inflammatory mediators by observing histopathological features associated with LPS-induced ALI. The rats pretreated with Lider-7 had significantly lower levels of IL-6 (at 3 and 6 h), and TNF-α (at 3, 6, 9, and 12 h). The current study showed that Lider-7 exerted a preventive effect against LPS-induced ALI, which appeared to be mediated by inhibiting the release of pro-inflammatory cytokines.

Effect of Channadselshi on voluntary alcoholic wistar rats / Монголын Анагаах Ухаан

INTRODUCTION:Alcohol (EtOH) use disorders (AUDs) represent a substantial public health problem worldwide. Over 76million people present with AUDs 2.5 million deaths were attributed to alcohol (World Health Organization,2010). Channadshelshi has been used for treatment of alcohol-related, liver disease and intoxication intraditional medicine.GOAL:To determine effect of channadshelshi on voluntary alcoholic wistar rats.MATERIALS AND METHODS:To examine acute toxicity of Channadselshi were used V.P.Prozorovsky express method (1978) andOECD (2001).Voluntary EtoH consumption measurement (two-bottle choice, intermittent access to EtOH paradigm inwistar rats). Water and 20% ethanol were presented in 200 mlgraduated plastic cylinders with stainlesssteeldrinking spouts.The experiment period was 10 weeks total. EtOH consumption was expressed asgrams of EtOH consumed per kilogram of body weight/day.Elevated plus maze. Anxiety associated with EtoH withdrawalwas measured on EPM The mazewaselevated 1 m above the floor and contained four 50 cm long, 10 cmwidearms arranged at rightangles. The closed arms had opaque walls 30cm high, extending the length of the arm. At the time of thetest, eachanimal was placed in the center of the maze facing an open arm andallowed to explore for a5 min session. During this 5 min test session, theanimal’s number of arm entries and time spent in eacharm per entry was recorded on a camera.Pentylenetetrazol (PTZ) 100 mg/kg dose used in this study was determined as the dose that inducedseizures and protection against mortality in miceThe experimental protocol was approved by the Ethics Committee of the MNUMS. (№ 14-11/1À)RESULTS:LD50 of Channadselshi extract was found to be LD50=2.58 (2.1-3.2) gr/kg by V.P.Prozorovsky expressmethod (1978). Channadselshi was no acute toxic by OECD (2001).Ten and four weeks oral administration of Channadselshi (200 mg/kg) significantly (P<0.01) decreasedthe consumption of ethanol and significantly (P<0.01) increased time spent in open arm (EPM) involuntary alcoholic wistar rat compare to control.The time (sec) of latency the generalized convulsion were significantly (P<0.01) increased ofChannadselshi compare to control. The percent of protection against mortality were 80% in PTZinduced seizure in mice.CONCLUSIONS:1. Our study showed that Channadselshi hasreduced consumption of ethanol in voluntary alcoholicwistar rat.2. Channadselshi has central nervous system protection effect against PTZ induced seizure in mice.

OF BIOLOGICAL ACTIVITY COMPOUNDS IN SOME / Монголын эм зүй, эм судлал

The aim of this study is to investigate biological activity compounds in Mongolian traditional medicinal plants, such as Leonurus Sibiricus L., Ephedra Przewalskii Stapt. and Lonicera Altaica Pall. In the phytochemical research, biological active substance was determined by using paper chromatography and thin-layer chromatography, on silica gel plats. We determined the total content of phenolic compound by the Folin–Ciocalteu method, total alkaloid, and tannin by titrimetric method and total fl avonoid by spectrophotometric method. The following biological active substances were detected: catechin, ephedrine, anthocyan and rutin. As a result of our research, it was determined that the total alkaloids was 0.02 percent, tannin was 9.03 percent in herba Ephedra Przewalskii Stapt , total phenolic compound was 3.3 percent in Leonurus Sibiricus L. and total fl avonoid was 6.6 percent in fruits Lonicera Altaica Pall. Key words: Leonurus Sibiricus L., Ephedra Przewalskii Stapt., Lonicera Altaica Pall., thinlayer chromatography, phytochemical INTRODUCTION Ephedra przewalskii is a species of Ephedra that is native to Central Asia, Mongolia,Pakistan, and parts of China ( Gansu, Inner Mongolia, Ningxia, Qinghai, and Tibet) [1][2]. Ephedra przewalskii is widely distributed in desert regions of Mongolia such as khobdo, Dornogovi, and Mongol Altai [3]. Ephedrae herba has been used in traditional Chinese, Mongolia and Japanese (Kampo) medicine from ancient times. In Mongolia, Ephedra przewalskii herba has traditionally been used by indigenous people for a variety of medicinal purposes, including treatment of asthma, hay fever, and the common cold [4]. Herba of Ephedra przewalskii are considered to be rich source of a large amount of bioactive substances like alkaloids, catechin, fatty oil and carbohydrate [3]. Leonurus Sibiricus L., is a member of the family Lamiaceae, is widely distributed in forested area and desert regions of Mongolia such as Khangai, Khuvsgul, Khentii Mongol Daguur, and Mongol Altai [5]. In traditional Mongolian medicine aerial parts of Leonurus Sibiricus L., used for treatment of diseases of heat reduction, removal of poisonous diseases, soothing and reducing hypertension. Aerial parts of Leonurus Sibiricus L.are considered to be rich source of a large amount of bioactive substances like fl avonoids, alkaloids, tannin and organic acids [5]. In mongolia has described four species of Lonicera Altaica Pall (Caprifoliaceae). Their habitats are khuvsgul, khangai, Khentii, khobdo, Mongolian altai, and depression of great lakes, mongol daguur and the Gobi-altai (three Beauty of gobi) region of the country. Lonicera altaica in Mongolian traditional medicine is reputed for its treatment of liver, stomach and cardiac disorders, Moreover, it is known for its rejuvenation action [6]. Fruit of Lonicera Altaica Pall. is considered a rich source of bioactive substances like fl avonoids, anthocyanins, vitamin C and amino acids [6].

CHEMICAL AND PHYSICAL STUDY OF THE “EGHOMON” SUPPLEMENTS / Монголын эм зүй, эм судлал

The biologically active food supplement products were produced in the form natural raw materials for powder, tablets, pellets, capsule, juice and extract preparation. Osteoporosis disease is increasing among the population of the world in recent years. Therefore, calcium supplement is used more often for prevention of this disease. Osteoporosis is now widely recognized as a public health problem since this disease, which increases bone fragility and thereby the risk of fractures, is associated with high mortality, morbidity and medical expenses throughout the world. We obtained new “Eghomon” supplement which reduces osteoporosis. We determined by quantitative analysis, the calcium ion and mineral composition is a crystal structure in this supplement. In the study, we used titrimetric, X-ray fl uorescence and X-ray diffractometer spectroscopy methods. The following mineral elements such as, Ca, P, Mg, Fe, Mn, Al, Na, K, Si, Ti were contained in the Eghomon supplement. Additionally, we determined the concentration of calcium ion 22.9 percent in the supplement. INTRODUCTION Mineral ions such as calcium, phosphorus, magnesium, copper, zinc, etc.., play a tremendous role in the metabolic processes of the human body, including tissue, fl uid, and bone. In each are they function to promote and maintain normal body function with a wide range of activities including assisting in body hydration, enzyme activation, maintenance of pH, as cofactors in blood coagulation, controlling neuromuscular excitability, as well as promoting the stability of bones and teeth, and many other functions. Calcium is the most abundant mineral in the human body, most of which (98%) is present in the skeleton. The concentration of calcium ion in the blood is very important for the development and preservation of strong bones and teeth. Absorption of too much calcium in the blood may lead to the leaching of calcium from the bones and teeth. The absorption of calcium is controlled, in part, by vitamin D. Growing children in particular need an ample supply of calcium for the development of strong bones. Milk, which is a good natural source of calcium and which is consumed primarily by children, is usually fortifi ed with vitamin D to promote calcium absorption. Similarly, outdoor recess for elementary school children is mandated by law in many states because exposure to sunshine allows the body to synthesize vitamin D. In later adulthood, particularly among women, the level of calcium in the blood may decrease to the point where calcium is removed from the bones and teeth to replace the blood calcium, making the bones and teeth much weaker and more susceptible to fracture. This condition is known as osteoporosis and can become very serious, causing shrinking if the skeleton and severe arthritis. For this reason, calcium supplements are often prescribed in an effort to maintain the proper concentration of calciumion in the blood. Although the results are not yet conclusive, recent reports indicate 100mg of calcium ion, taken orally on a daily basis, may serve to prevent osteoporosis. Typically, such calcium supplements consist of calcium carbonate, calcium citrate and calcium lactate. MATERIALS AND METHODS We conducted used in the study of natural biologically active food supplements Eghomon. The researchers invented new Eghomon supplement in the institute of traditional medicine and technology of Mongolia. Eghomon is composed from horse bones and eggshells. It is a calcium preparation.

The effect of bee venom on kidney function in alloxan induced diabetic rabbits / Монголын Анагаах Ухаан

Background Diabetes is a metabolic disorder that is characterized by chronic high blood glucose levels that causes complications in the eyes, kidneys, heart, vessels and nerves. Currently diabetic nephropathy is the most significant long-term complications in terms of morbidity and mortality for individual patients with diabetes. Honey bee venom can be considered as a natural remedy for diabetes due to its blood glucose levels lowering and lipid-regulating effect on diabetic rabbits. Aim The aim of this study was to investigate the effect of Mongolian honey bee venom (Apis mellifera) on renal dysfunction in alloxan induced diabetic rabbits. Material and Method Twenty two Chinchilla rabbits were divided into three groups: control (n=6), diabetic (n=8), and bee venom treated (n=8). The diabetic group was injected with 5% solution of Alloxan monohydrate 100 mg/kg intravenously behind the ear for 2 minutes to induce diabetes. The bee venom treated group received a bee sting (a sting contains 0.2-0.5 ml of bee venom) on their hind paw every day after their diagnosis of diabetes. Result Bee venom treatment (BVT) led to the following changes: compared to the diabetic group, the bee venom treated group’s blood glucose levels lowered between 14.9% and 26.5%; the plasma creatinine and urea levels were decreased respectively by 19,8% and 14.8%. Blood urea nitrogen (BUN) levels were reduced by 14.8%. Conclusion: Treatment with Mongolian bee venom lowered the blood glucose levels and prevents the renal dysfunction in alloxan induced diabetic rabbits

Pharmacological study of zomoshin-6 tan / Монголын Анагаах Ухаан

IntroductionNowadays the risk factors for thrombosis include blood stasis, vessel wall injury, and hypercoagulability, as proposed by Virchow over 150 years ago. We chose to study affect of Zomoshin 6 tan to the model of thrombosis formed in experimental animal. It was written in books and sudar that it has action of treating some type’s disease such as blood diluting, meeting frozen blood and treating some gynecological disease.PurposeTo study affect of Zomoshin-6 tan deep vein thrombosis model formed in experimental animal.Material and Methods30 male rats of wistar bread with 180-220 gram of weight for control group, experimental group or Zomoshin-6 tan and comparative group Warfarin. Thirty rats were equally divided into 3 groups: Group 1 received saline alone, Group 2 received Zomoshin-6 (200 mg/kg), and Group 3 received Warfarin as a positive control (0.25 mg/kg), seven days prior to the assessment of thrombus formation. Thrombus formation was also assessed histopathologically. Thrombi were detected in all rats after experimentallyinduced thrombosis.ResultsHistological analysis demonstrated the presence of thrombosis in the interior vena cava (IVC) of the control group, which contained fibrin, erythrocytes, and leucocytes and obstructed the lumen. Only a small amount of fibrin clot, containing a few leucocytes and large numbers of erythrocytes, were observed in the Zomoshin-6-treated group. The thrombus formed in the IVC of Warfarin-treated animals consisted of fibrin clot, which was mostly attached to the wall, with few leucocytes but abundant erythrocytes. These findings suggest that Zomoshin-6 is an effective antithrombotic agent.Conclusion:Zomoshin-6 tan has an action of inhibing thrombosis forming of vein in experimental animal.

The phytochemic and pharmakologic activity extract of malbus in kidney damage / Монголын Анагаах Ухаан

BackgroundThese study made us to investigate the drug row material of Mongolian traditional medicine such us Tribulus terrestris, Malva neclecta Wall and Eriocheir sinensis compounded preparation named “Malbus”. Investigated us preparations steel using for treatment of kidney and urinetract deseases, it is one of effective in Mongolian traditional drug preparations.Material and MethodsThe experimental protocol was approved by the Ethics Review Committee at the Ministry Health of Mongolia. In research we used 20 healthy mice and 60 Wistar rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Producttion Corporation and pathological laboratory of Institute Veterinary medicine. Chemically acting substances is steroid saponin content in Malbus compound was detected by using thin layerchromatography (TLC) and its value was determined by UV-spectrophotometry. The acute oral toxicity study was according to the method Prozorovsky (1978). The toxic nephrosis was produced using Gentamicin (80 mg/kg) rats by using Neugarten’s method (1983).ResultsThe ethanolic extract results of the phytochemical investigations showed that conteined in the “Malbus” determined the presence of bioactive substances such as flavonoids, steroid saponins. The ethanolic extract of Malbus was found to be LD50 of 16.3 g/kg. Toxic nephrosis was induced in Wistar rats administered preparation Malbus dose 80 mg/kg, 160 mg/kg, and 240 mg/kg. Blood levels of creatinine, uric acid, and urea were siginificantly reduced by Malbus treatment compared tocontrol. Histological study revealed that Malbus was effective for treatment of nephritis in rats induced by gentamicin.Conclusions:1. The ethanolic extract of “Malbus” compound determined the presence of bioactive substances such as flavonoids, steroid saponins.2. LD50 of Malbus 16.3 g/kg, the preparation is has nephroprotective effect on experimental Gentamicine induced nephrosis in rats.

Аnti-inflammatory activities of traditional Mongolian drug garidi-5 / Монголын Анагаах Ухаан

Introduction. Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz., Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its analgesic and anti-inflammatory activity.Materials and Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind paw was injected with 0.1 ml of vehicle.Results. The Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi- 5 (80 and 200 mg/kg) at 4 h when compared to the control group (Tab.1). In assay data, the TNF-α, IL-1β and IL-6 secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 signifi cantly reduced serum secretion of inflammatory mediatorsas compared to vehicle group (Tab. 2).Conclusion. In conclusion, Traditional drug Garidi-5 have anti-in flammatory properties. The potential efficacy of Garidi-5 to treat inflammation is based in a part on the hy pothesis that it will suppress the proinflammatory cytok ines resulting in less oedema.

Аnti-inflammatory activities of traditional mongolian drug garidi-5 / Монголын Анагаах Ухаан

Introduction. Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz. Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its analgesic and anti-inflammatory activity.Materials and Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind pawwas injected with 0.1 ml of vehicle.ResultsThe Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi-5 (80 and 200 mg/kg) at 4 h when compared to the control group (Tab.1). In assay data, the TNF-α, IL-1β and IL-6 secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 signifi cantly reduced serum secretion of inflammatory mediators as compared to vehicle group (Tab. 2). ConclusionIn conclusion, Traditional drug Garidi-5 have anti-in flammatory properties. The potential efficacy of Garidi-5 to treat inflammation is based in a part on the hy pothesis that it will suppress the proinflammatory cytok ines resulting in less oedema.

The chemical and pharmacological study of the new drug "Calvacin" / Монголын Анагаах Ухаан

Background: Wound healing mechanisms still unclear. Wounds are physical injuries that result in an opening or breaking of the skin. Proper healing of wounds is essential for the restoration of disrupted anatomical continuity and disturbed functional status of the skin. This is a product of the integrated response of several cell types to injury. Wound healing is a complex process that results in the contraction and closure of the wound and restoration of a functional barrier.Goal: The purpose of this study was to determine burn wound healing activity of Calvacin. Materials and Methods:We used in study as colored reaction thin layer chromatography (TLC) and UV-spectrophotometer methods to determine active compounds in the three component drug "Calvacin".In research were inducted 20 healthy white mice and 40 rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Production Corporation and Institute of Veterinary medicine of pathological laboratory. The study protocol was approved by the Ethics Committee of the Ministry of Health.Results:Results of the phytochemical investigations showed that contained in the new drug "Calvacin" determined the presence of bioactive substances such as flavonoids, saponin, tannin, curcuminoids, organic acids and minerals. Substance was absorbed 25.7% in 40 grade ethanol, 27.1% in 70 grade, 22.2% in 96 grade and 19.6% in sterile water. We was established quality quantities of Calvacin in the drug amount summary flavonoids 1% and determined by UV spectrophotometer method. Proceeding from the absorption maximum of the substances analyzed the wave length of detection was determined as 370nm. In the result of mineral study of the "Calvacin", Ca

The study of immunostimulating effect bee venom / Монголын Анагаах Ухаан

Introduction In 19th century, researchers proved at biochemical level the healing properties of bee products such as bee venom, honey, royal jelly, pollen, propolis and wax. The object of our research is the Apis cerena’s venom properties1-2. Asiatic honey bee or Apis cerana is small honey are small honey bees of southern and southeastern Asia, such as China, India, Japan, Malaysia, Nepal, Bangladesh and Papua New Guinea3. This species is also known as the Himalayan hive honeybee. This species is the sister species of Apis koschevnikovi, and both are in the same sub¬genus as the Western (European) honey bee, Apis mellifera4. Goal The purpose of our research is to study property and potential of bee venom and its effect on immune system. Heal¬ing property of Apis cerana was high. This study proves that bee venom therapy stimulates immunity. Materialis and Methods The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. We put a bee sting to 0.5 ml of 10% red blood cell (RBC) solution and measured time of heamolysis to de¬fine bee venom potential/capability by Shkenderov S., Ivanov Ts., (1985) method. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) methodology of studying effects on immune system, we have stung bee venom to 23 mice on the acupuncture point of hind paw every other day in total 3 times. On third day of the experiment, we in¬jected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre. Results Potential of bee venom is determined by speed of heamolysis when bee sting is placed in the 0.5 ml of 10% RBC solution. If we place one bee sting into 1ml of RBC solution then the speed of heamolysis is 46 seconds, when two stings are place speed is 38 seconds and when 3 stings placed then time is 30. Compare to usual speed of heamolysis which is 60 seconds, change in time depending on the number of bee stings proves the effectiveness of bee venom (Table 1). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5*106/ml. BVT group’s number of spleen cells was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutina¬tion titre of BVT group was 1.13 times higher (BVT group 54.86±19.95%; control group 50±8.83%, p<0.05) and this indicates that BV has immunity stimulating effect. Conclusions From our experiment we can conclude the following 1. Apis mellifera’s bee venom has high treating effect. 2. Bee venom therapy has immunity stimulating activity.