Research progress on carbapenem-resistant Klebsiella pneumoniae infection in organ transplantation / 器官移植

Klebsiella pneumoniae (KP) is a common conditional pathogen, and also one of the common pathogens causing infection in immunocompromised patients, with its infection rate increasing year by year. Carbapenem antibiotics are effective drugs to control KP infection. But with the widespread use of carbapenem antibiotics, carbapenemresistant Klebsiella pneumoniae (CRKP) appears and increases year by year. Organ transplant recipients are at high risk of CRKP infection due to the suppressed immune system. Once drug-resistant bacteria infection occurs, it is often difficult to control and the survival rate of transplant organs is reduced, which brings great challenges to clinical treatment. In this article, the current status and treatment progress of CRKP infection in organ transplantation are summarized.

Exploration on online teaching of Geriatrics during the COVID-19 pandemic / 中华医学教育探索杂志

The opening of universities and colleges has been postponed due to the outbreak of COVID-19. In order to fully guarantee the normal teaching order and achieve the goal of "classes suspended but teaching and learning continues", Central South University has adopted online teaching in the spring semester of 2020. The Geriatrics teaching team of Xiangya Hospital have actively responded to the school's call and carried out online teaching course. This paper summarizes the experience in the preliminary organization, process implementation, education management and teaching evaluation of the Geriatrics online course in order to provide reference for the teaching reform of this course.

Five-case report for transvenous epicardial pacemaker implantation via coronary sinus in patients after prosthetic tricuspid valve replacement / 中南大学学报(医学版)

Five patients after prosthetic tricuspid valve, who received pacemaker implantation via coronary sinus during Oct, 2011 and Jul, 2014, were enrolled. Pacemakers were implanted via coronary vein in 5 patients without complications. The stimulation thresholds keep stable and symptoms (such as short breath and fatigue) were disappeared during the follow-up. For patients after tricuspid valve replacement, implantation of pacemaker via coronary sinus provides a safe and invasive approach and avoids opening the chest again.

Phototaxis effect of Oncomelania hupensis hupensis and O . h . robertsoni-- a comparative study / 中国人兽共患病学报

The aim of this study was to determine the differences in phototaxis between Oncomelania hupensis (O .h .) hupensis and O .h .robertsoni as well as four related geographic strains of male and female snails and Schistosoma japonicum-infected and-uninfected snails .Field collection of O . hupensis was performed in S . j aponicum-endemic areas of Xingzi and Yushan counties in Jiangxi Province ,China ,as well as Tianquan and Pengshan counties in Sichuan Province ,China .Infected and uninfected snails were screened using the cercariae escaping method ,with artificial separation of male and female snails . The experiment was carried out in an in-house constructed dark phototaxis experiment box using white light as the main source of light at the most suitable light intensity .Under a light intensity of 1 500 Lux for 30 minutes ,all of the snails from the experimental group showed obvious phototaxis .The statistical analysis showed no significant difference in the phototaxis index between male and female snails ,and there was also no significant difference in the phototaxis index between different geograph-ical strains of the same subspecies of snails .A significant difference in phototaxis was observed between the two subspecies during the 30-minute experimental period :O .h .hupensis displayed a higher phototaxis index than that of O .h .robertsoni , whereas S .japonicum-infected snails showed a lower phototaxis index than that of uninfected snails .A difference in phototaxis was found between different subspecies of O .hupensis and within the same subspecies .All uninfected snails displayed the same phototaxis index .When snails were infected with S .japonicum ,phototaxis reduced .

Dendritic cells and coronary collateral circulation in coronary heart disease / 中南大学学报(医学版)

OBJECTIVE@#To determine the relationship between the number,phenotype and functional status of dendritic cells (DCs) and coronary collateral circulation (CCC) in coronary heart disease (CHD).@*METHODS@#Forty patients with severe coronary stenosis were recruited and divided into a CCC formation group (Group A, n=22) and a non-CCC formation group (Group B, n=18). Density gradient centrifugation was applied to separate the mononuclear cells (MNCs) from coronary artery blood samples, and MNCs were cultured and proliferated in vitro. The morphology of DCs was observed under converted microscope. The number of harvested cells and DCs was counted by hematocytometer. Flow cytometry was applied to investigate the phenotype and the mean fluorescence intensity (MFI). Mixed lymphocyte reaction was used to test the function of DCs to stimulate the proliferation of T lymphocytes. Stimulation index (SI) was calculated and compared.@*RESULTS@#(1) After in vitro proliferation, DCs were cultured successfully from the mononuclear cells from coronary artery blood samples and the morphology of DCs was not different in the 2 groups. (2) The number of mononuclear cells (MNC no) was (3.95+/-1.41)*10(6), in the CCC group and (2.76+/-0.92)*10(6) in the non-CCC group. The MNC number was significantly increased in the CCC group (P=0.003). (3) The number of DCs was (1.54+/-0.96)*10(6) in the CCC group, and (0.99+/-0.46)*10(6) in the non-CCC group (P=0.033). (4)There was no statistical significance in the percent of CD1a+, CD1a+CD80+, CD1a+CD83+, CD1a+CD86+ cells, and MFI in the 2 groups (P>0.05). (5) SI was 4.96+/-2.30 in the CCC group, whereas 2.66+/-1.04 in the non-CCC group. The SI in the CCC group increased significantly(P=0.0003).@*CONCLUSION@#In CHD patients with severe coronary stenosis, patients with CCC formation have higher number of DCs and stronger potential of T lymphocyte stimulation.

Changes of the cardiac morphology and size after transcatheter closure of atrial septal defect / 中国医师杂志

Objective To evaluate the changes of the right atrium,right ventriculum,left atrium and left ventriculum after transcatheter closure of atrial septal defect(ASD) during a short to mid-term follow-up.Methods The right ventricular end-diastolic anterior-posterior diameter(RVEDD),right atrial long diameter(RADl),right atrial transverse diameter(RADt),left ventricular end-diastolic ante-posterior diameter(LVEDD),left ventricular end-diastolic volume(LVEDV) and left atrial anterior-posterior diameter(LAD) in 36 patients with secundum ASD were measured before ASD closure,after 3 days,3 months and 6 months of ASD closure with transthoracic echocardiography(TTE).Results RVEDD,RADl and RADt were significantly decreased,while LVEDD,LVEDV and LAD significantly increased 3 days after ASD closure.During 3 months follow-up,RVEDD,RADl and RADt continuously became smaller;LVEDD,LVEDV and LAD continuously became larger.At 6 months,RVEDD was significantly smaller and LVEDD,LVEDV were significantly larger than those at 3 months.No remarkable difference of RADl,RADt and LAD was found between 6 months and 3months follow-up.Conclusion Transcatheter closure of ASD not only decreases the preload of right heart and causes right atrium and right ventriculum become smaller,but also improves the geometry of left heart and causes the narrowed left atrium and left ventriculum gradually return to almost normal status.

Direct PTCA of acute myocardial infarction-the relationship between type of balloon catheter and reperfu-sion arrhythmia / 中国介入心脏病学杂志

The relationship between type of balloon catheter and reperfusion arrhythmia (RA) of 97 patients with acute myocardial infarction (AMI) ,who had received direct PTCA has been studied. The incidence of RA in common balloon catheter treated group is 55. 4%,while that in perfusion balloon catheter treated group is 34. 4% and the extent of RA is less severe. During direct PTCA, perfusion balloon catheter can reduce the incidence and severity of RA.