Effects of Polysaccharide from Dendrobium officinale Kimura et Migo on the Reproductive Damage in Male Mice Induced by Cisplatin / 中国药学杂志

OBJECTIVE: To study the effects of polysaccharides from Dendrobium candidum Kimura et Migo (DOP) on reproductive damage in male mice induced by cisplatin. METHODS: Forty male Kunming mice were randomly divided into eight groups: control group, DOP low-dose group (LD-DOP), DOP medium-dose group (MD-DOP), DOP high-dose group (HD-DOP), cisplatin group, cisplatin-low-dose DOP group (cisplatin-LD-DOP), cisplatin-medium-dose DOP group (cisplatin-MD-DOP), and cisplatin-high-dose DOP group (cisplatin-HD-DOP), 5 mice in each group. The cisplatin group and cisplatin-LD/MD/HD-DOP group were intraperitoneally injected with 2.5 mg•kg-1•d-1 cisplatin for 5 d, control group and LD/MD/HD-DOP group were intraperitoneally injected with equal volume of saline. After 24 h of the last injection, the 3 DOP groups and cisplatin-DOP group were intragastrically administrated with 100 mg•kg-1•d-1 DOP (low-dose), 200 mg•kg-1•d-1 DOP (medium-dose) and 400 mg•kg-1•d-1 DOP (high-dose), control group and cisplatin group were intragastrically administrated with equal volume of saline. The mice were sacrificed after DOP treatment for 35 d, and were sampled to evaluate effects of DOP on the number of sperm, sperm quality, histopathology, as well as oxidative stress of testicular tissue in mice. RESULTS: Compared with cisplatin group, index of testis and epididymis, the number of sperm, sperm motility and viability were significantly increased (P<0.05 or P<0.01), and sperm abnormality rate was significantly decreased (P<0.01) in cisplatin-LD/MD/HD-DOP group. The SOD activity was significantly increased (P<0.01), and MDA content was significantly decreased (P<0.01) in testicular tissue. Meanwhile, histopathologicla damage of testicular tissue was significantly improved in cisplatin-LD/MD/HD-DOP group. Compared with cisplatin-LD-DOP group mice, the number of sperm was increased and sperm quality was improved, oxidative damage of the testicular tissue and histopathological damage were significantly improved in cisplatin-MD-DOP group and cisplatin-HD-DOP group mice. CONCLUSION: DOP could significantly increase sperm numbers as well as improved sperm quality, and promote the antioxidative ability of testicular tissue in cisplatin model mice, the result shows that DOP has preventing effects on reproductive damage of model mice in dose-dependent manner.

Effects of alcohol on benign prostate hyperplasia induced by testosterone propionate in mice / 中国应用生理学杂志

OBJECTIVE@#To study the effects of alcohol administration on benign prostate hyperplasia(BPH) and the reproductive toxicity during development of benign prostate hyperplasia.@*METHODS@#Seventy adult male Kunming mice were randomly divided into seven groups:control (group CON), negative control (group NC, injected subcutaneously with soybean oil, 25 mg/(kg·d), intragastric administration of distilled water, 7.5 ml/(kg·d)), alcohol for 7 and 21 days (group AL7 and AL21, intragastric administration with wine of 50% alcohol, 7.5 ml/(kg·d)), testosterone propionate for 7 and 21 days (group TP7 and TP21, injected subcutaneously with testosterone propionate, 25 mg/(kg·d)), testosterone propionate+alcohol for 7 days (group TP+AL7, injected subcutaneously with testosterone propionate, 25 mg/(kg·d), and intragastric administration with wine of 50% alcohol, 7.5 ml/(kg·d)),10 mice in each groups. Twenty-four hours after the last administration, mice were sacrificed. The indexes of prostate and testis and the parameters of sperm were determined in mice. The levels of free radicals, antioxidation and histopathological changes in testis and prostate were determined.@*RESULTS@#Compared with the control, TP7d group, AL7 and AL21d groups, the prostate coefficient of TP + AL7d group was increased significantly and the quantity and quality of sperm were decreased significantly (0.05).@*CONCLUSIONS@#The typical BPH state could be induced after 7-day treatment of testosterone propionate and alcohol. The testicular and sperm were damaged which enhanced the oxidative stress in reproductive system. The results indicated that alcohol could significantly promote the prostate hyperplasia induced by testosterone propionate in mice.

Detection and typing of human papillomavirus by a GeXP based multiplex PCR assay / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To establish a new and rapid GeXP based multiplex PCR assay for the detection and typing of human papillomavirus 6, 11, 31, 33 and 52.</p><p><b>METHODS</b>Nucleotide sequences of HPV6, HPV11, HPV31, HPV33 and HPV52 from NCBI were obtained and compared. Genotype-specific primers were then designed and the sensitivity and specificity of multiple PCR assay was evaluated. Optimized assay was further validated with 30 clinical specimens collected from the cervical secretions of patients.</p><p><b>RESULTS</b>A GeXP based multiplex PCR was developed for sensitive detection and reliable differentiation of five HPV genotypes (HPV6, 11, 31, 33 and 52),</p><p><b>CONCLUSION</b>A GeXP based multiplex PCR assay is demonstrated to be a new and rapid technique for simultaneous detection and typing of 5 different human papillomaviruses.</p>

Colorimetric detection of HPV6 and HPV16 by loop mediated isothermal amplification / 病毒学报

A simple, rapid and sensitive colorimetric loop mediated isothermal amplification (LAMP) method was established to detect HPV6 and HPV 16 respectively. The method employed a set of four specially designed primers that recognized six distinct sequences of HPV6-E6 or HPV16-E7 for amplification of nucleic acid under isothermal conditions at 63 degrees C for one hour. The amplification process of LAMP was monitored by the addition of HNB (hydroxy naphthol blue) dye prior to amplification. A positive reaction was indicated by a color change from violet to sky blue and confirmed by real-time turbidimeter and agarose electrophoresis. Thirteen cervical swab samples having single infection with 13 different HPV genotypes were examined to evaluate the specificity. A serial dilution of a cloned plasmid containing HPV-E6 or HPV-E7 gene was examined to evaluate the sensitivity. The results showed that no cross-reaction with other HPV genotypes was observed. The colorimetric LAMP assay could achieve a sensitivity of 1000 copies, 10-20 times lower than that of real-time PCR. The assay was further evaluated with 62 clinical specimens and consistent results were obtained compared with the detection using Kai Pu HPV Genotyping Kit. We concluded that this colorimetric LAMP assay had potential usefulness for the rapid screening of the HPV6 or HPV16 infection in the laboratories and hospitals of provincial and municipal region in China.