Immunological parameters on prophase of severe hepatitis B / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study cellular and humoral immune status on prophase of severe hepatitis B (PSHB).</p><p><b>METHODS</b>56 cases of PSHB patients, 40 cases of chronic hepatitis B (CHB) patients and 20 cases of healthy volunteers were enrolled for detection of CD3+, CD4+, CD8+ and CD3-/CD19+ (B cells) lymphocyte subsets in peripheral blood by flow cytometry. Serum IgG and complement C3 was detected by immunoturbidimetry and analyzed statistically.</p><p><b>RESULTS</b>Compared with CHB group and healthy control group, percentage of lymphocyte subsets CD8+ were significantly lower in PSHB group (P < 0.01 or P < 0.05). While the percentage of lymphocyte subsets CD4+ and ratio of CD4+/CD8+ in PSHB group was obviously higher than those in CHB group (P < 0.+01 or P < 0.05). In addition, There was no significant difference on the percentage of B cell and level of serum IgG between PSHB group and CHB group (P > 0.05, while the level of serum complement C3 in PSHB group were significantly lower than those in CHB group and healthy control group (P < 0.01, P < 0.05).</p><p><b>CONCLUSION</b>PSHB has a certain degree of cellular immune dysfunction, which characterized by cellular immune function hyperfunction and humoral immune suppression.</p>

The characteristics of immunocompetent cells in peripheral blood on prophase of severe hepatitis B / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To analyze the characteristics of immunocompetent cells in peripheral blood on prophase of severe hepatitis B (PSHB).</p><p><b>METHODS</b>48 cases of PSHB patients, 35 cases of chronic hepatitis B (CHB) patients and 20 cases of healthy volunteers were enrolled for detection of CD3+, CD3+/ CD4+, CD3+/CD8+ and CD4+/CD25+/CD45+ lymphocyte subsets in peripheral blood by flow cytometry. The absolute numbers of each lymphocyte subset were calculated and analyzed statistically. Results Compared with CHB group and healthy control group, The absolute numbers of circulating CD3+, CD8+ T cells and CD4+ CD25+ regulatory T cells (Tregs) were significantly lower in PSHB group( P < 0. 01 or P < 0.05). There was no significant difference on the absolute numbers of circulating CD4+ T cells between PSHB group and CHB group (P > 9.05), while the percentage of lymphocyte subsets CD4+ in PSHB group was significantly higher than that in CHB group (P < 0.05). In addition, CD4+/CD8+ ratio in PSHB were significantly higher than those in the CHB group and healthy control group (P < 0.01 or P < 0.05).</p><p><b>CONCLUSION</b>PSHB has a certain degree of cellular immune dysfunction, which characterized by CD4+ T cells dominated and the decline of absolute numbers of CD8+ T cells and CD4+ CD25+ Tregs.</p>