Alkaloid constituents from the fruits of Flueggea virosa / 中国天然药物

Three new indole alkaloids, flueindolines A-C (1-3), along with nine known alkaloids (4-12), were isolated from the fruits of Flueggea virosa (Roxb. ex Willd.) Voigt. Compounds 1 and 2 are two new fused tricyclic indole alkaloids possessing an unusual pyrido[1, 2-a]indole framework, and 3 presents a rare spiro (pyrrolizidinyl-oxindole) backbone. Their structures with absolute configurations were elucidated by means of comprehensive spectroscopic analysis, chemical calculation, as well as X-ray crystallography. Chiral resolution and absolute configuration determination of the known compounds 4, 10, and 11 were reported for the first time. The hypothetical biogenetical pathways of 1-3 were herein also proposed.

Phytochemical study ofand its biological activities

Sixteen compounds, including two new natural products (and), were obtained from the twigs of. The structures were elucidated based on NMR, MS, IR data and optical rotation values. Compounds,,anddisplayed moderate antibacterial activities against clinical isolates; compounds,,,andprotected neural cells against oxidative stress; and compoundsandexhibited anti-acetylcholinesterase activity.

Identification of Swertia mussotii and its adulterant Swertia species by 5S rRNA gene spacer / 中国中药杂志

<p><b>OBJECTIVE</b>This research focused on analyzing the differences of 5S rRNA gene spacer sequences on Swertia mussotii and its commonly used adulterants, including S. franchetiana, S. wolfangiana and S. chirayita.</p><p><b>METHOD</b>DNA was extracted from the collected Swertia samples. 5S rRNA intergenic spacers were amplified by PCR, sequenced and analyzed.</p><p><b>RESULT</b>5S rRNA gene spacer sequences were different between S. mussotii and its other three adulterants. Sequence divergence among species ranged from 30.6% to 65.0%.</p><p><b>CONCLUSION</b>5S rRNA spacers may be used as molecular authentication markers to differentiate S. mussotii and other commonly used Swertia adulterants. This result provides reliable and simple reference for the authentication of Swertia genus species.</p>

Chemical constituents and anti-tuberculosis activity of root of Rhodiola kirilowii / 中国中药杂志

The chemical constituents of Rhodiola kirilowii were separated and purified by repeated column chromatography on silica gel, RP - 18, Sephadex LH -20 and semi-preparative HPLC. Each compound was characterized by spectroscopic and physical data. Twelve compounds have been purified and identified to be beta-sitosterol (1), tyrosol (2), trans-hydroxycinnamic acid (3), geranyl beta-glucopyranoside (4), neryl beta-glucopyranoside (5), hexyl beta-glucopyranoside (6), gallic acid (7), (-) -epigallocatechin gallate (8), rhodiolgin (9), isolariciresinol-9-O-beta-glucopyranoside (10), rhodiooctanoside (11), and sacranoside B (12). Among them, compounds 3, 6, 9-12 were isolated from Rhodiola kirilowii for the first time; Compounds 4 and 5 were obtained for the first time from the genus Rhodiola. The in vitro activities against Macobacterium tuberculosis (ATCC 27294) of its extracts and pure components were evaluated by testing their MIC (minimal inhibitory concentration) and MBC (minimal bactericidal concentration). The 80% (a. q.) EtOH extract, EtOAc-soluble extract, compounds 7 and 8 exhibited in-vitro inhibitory and bactericidal activities against Macobacterium tuberculosis in different extent.

Determination of morusin in cortex mori / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the contents of morusin in Sangbaipi (Cortex Mori) of different commercial grades and species and obtained from different regions.</p><p><b>METHOD</b>Contents of morusin were determined by HPLC on an Alltima C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (58:42) as mobile phase. UV detection was conducted at 270 nm.</p><p><b>RESULT</b>The amounts of morusin were found to vary among samples of Cortex Mori bearing a yellow-brown outer layer and those without the yellow-brown cork layer. The morusin contents were also related to the thickness of Cortex Mori.</p><p><b>CONCLUSION</b>The method was simple, reproducible and reliable. It can be applied to the quality assessment of Cortex Mori.</p>